Contrast enhancement of laser speckle skin image: Use of optical clearing agent in conjunction with micro-needling

Taeyoon Son, Jinhee Yoon, Chang Yong Ko, Yong Heum Lee, Kiwoon Kwon, Han Sung Kim, Kyoung Joung Lee, Byungjo Jung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Laser speckle imaging modality is one of widely used methods to evaluate blood flow because of its simplicity. However, laser speckle image has a limitation in the evaluation of subcutaneous blood flow due to its low contrast perfusion image. Various methods have been tried to enhance the perfusion image contrast. Such methods presented positive results in some degree. However, it could not be fundamental solutions due to low penetration depth of lasers restricted by optical tissue scattering property. This study suggests a method to enhance the perfusion image contrast of laser speckle imaging modality by increasing the penetration depth of lasers. An optical clearing agent (glycerol) was topically applied on skin treated with micro-needle roller in order to reduce the time period of optical tissue clearing and therefore, enhance the penetration depth of laser. In this study, we investigated the effect of glycerol and micro-needling methods in the contrast enhancement of laser speckle perfusion skin image and presented the results of in-vitro and in-vivo animal experiment.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
DOIs
Publication statusPublished - 2008 Apr 21
EventPhotonic Therapeutics and Diagnostics IV - San Jose, CA, United States
Duration: 2008 Jan 192008 Jan 19

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6842
ISSN (Print)1605-7422

Other

OtherPhotonic Therapeutics and Diagnostics IV
CountryUnited States
CitySan Jose, CA
Period08/1/1908/1/19

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All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Son, T., Yoon, J., Ko, C. Y., Lee, Y. H., Kwon, K., Kim, H. S., Lee, K. J., & Jung, B. (2008). Contrast enhancement of laser speckle skin image: Use of optical clearing agent in conjunction with micro-needling. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE [68420E] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6842). https://doi.org/10.1117/12.764308