Controllable delivery of non-viral DNA from porous scaffolds

Jae Hyung Jang, Lonnie D. Shea

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

The inductive approach to tissue engineering combines three-dimensional porous scaffolds with drug delivery to direct the action of progenitor cells into a functional tissue. We present an approach to fabricate scaffolds capable of controlled, sustained delivery by the assembly and subsequent fusion of drug-loaded microspheres using a gas foaming/particulate leaching process. DNA-loaded microspheres were fabricated from the copolymers of lactide and glycolide (PLG) using a cryogenic double emulsion process. Microspheres were fabricated in four populations with mean diameters ranging from 12.3 μm to 92.5 μm. Scaffolds fabricated by fusion of these microspheres had an interconnected open pore structure, maintained DNA integrity, and exhibited sustained release for 21 days. Control over the release was obtained through manipulating the properties of the polymer, microspheres, and the foaming process. Decreasing the microsphere diameter or the molecular weight of the polymer used for microsphere fabrication led to increased rates of release from the porous scaffold. Additionally, increasing the pressure of CO2 increased the DNA release rate. The ability to create porous polymer scaffolds capable of controlled release rates may provide a means to enhance and regulate gene transfer within a developing tissue, which will increase their utility in tissue engineering.

Original languageEnglish
Pages (from-to)157-168
Number of pages12
JournalJournal of Controlled Release
Volume86
Issue number1
DOIs
Publication statusPublished - 2003 Jan 9

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

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