Controlled Delivery of Extracellular ROS Based on Hematoporphyrin-Incorporated Polyurethane Film for Enhanced Proliferation of Endothelial Cells

Min Ah Koo, Bong Jin Kim, Mi Hee Lee, Byeong Ju Kwon, Min Sung Kim, Gyeung Mi Seon, Dohyun Kim, Ki Chang Nam, Kang Kyun Wang, Yong Rok Kim, Jong Chul Park

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The principle of photodynamic treatment (PDT) involves the administration of photosensitizer (PS) at diseased tissues, followed by light irradiation to produce reactive oxygen species (ROS). In cells, a moderate increase in ROS plays an important role as signaling molecule to promote cell proliferation, whereas a severe increase of ROS causes cell damage. Previous studies have shown that low levels of ROS stimulate cell growth through PS drugs-treating PDT and nonthermal plasma treatment. However, these methods have side effects which are associated with low tissue selectivity and remaining of PS residues. To overcome such shortcomings, we designed hematoporphyrin-incorporated polyurethane (PU) film induced generation of extracellular ROS with singlet oxygen and free radicals. The film can easily control ROS production rate by regulating several parameters including light dose, PS dose. Also, its use facilitates targeted delivery of ROS to the specific lesion. Our study demonstrated that extracellular ROS could induce the formation of intracellular ROS. In vascular endothelial cells, a moderated increase in intracellular ROS also stimulated cell proliferation and cell cycle progression by accurate control of optimum levels of ROS with hematoporphyrin-incorporated polymer films. This modulation of cellular growth is expected to be an effective strategy for the design of next-generation PDT.

Original languageEnglish
Pages (from-to)28448-28457
Number of pages10
JournalACS Applied Materials and Interfaces
Volume8
Issue number42
DOIs
Publication statusPublished - 2016 Oct 26

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

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