Controlled Delivery of Extracellular ROS Based on Hematoporphyrin-Incorporated Polyurethane Film for Enhanced Proliferation of Endothelial Cells

Min Ah Koo, Bong Jin Kim, Mi Hee Lee, Byeong Ju Kwon, Min Sung Kim, Gyeung Mi Seon, Dohyun Kim, Ki Chang Nam, Kang Kyun Wang, Yong Rok Kim, Jong Chul Park

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The principle of photodynamic treatment (PDT) involves the administration of photosensitizer (PS) at diseased tissues, followed by light irradiation to produce reactive oxygen species (ROS). In cells, a moderate increase in ROS plays an important role as signaling molecule to promote cell proliferation, whereas a severe increase of ROS causes cell damage. Previous studies have shown that low levels of ROS stimulate cell growth through PS drugs-treating PDT and nonthermal plasma treatment. However, these methods have side effects which are associated with low tissue selectivity and remaining of PS residues. To overcome such shortcomings, we designed hematoporphyrin-incorporated polyurethane (PU) film induced generation of extracellular ROS with singlet oxygen and free radicals. The film can easily control ROS production rate by regulating several parameters including light dose, PS dose. Also, its use facilitates targeted delivery of ROS to the specific lesion. Our study demonstrated that extracellular ROS could induce the formation of intracellular ROS. In vascular endothelial cells, a moderated increase in intracellular ROS also stimulated cell proliferation and cell cycle progression by accurate control of optimum levels of ROS with hematoporphyrin-incorporated polymer films. This modulation of cellular growth is expected to be an effective strategy for the design of next-generation PDT.

Original languageEnglish
Pages (from-to)28448-28457
Number of pages10
JournalACS Applied Materials and Interfaces
Volume8
Issue number42
DOIs
Publication statusPublished - 2016 Oct 26

Fingerprint

Hematoporphyrins
Polyurethanes
Endothelial cells
Reactive Oxygen Species
Oxygen
Photosensitizing Agents
Photosensitizers
Cell proliferation
Dosimetry
Cells
Tissue
Singlet Oxygen
Cell growth
Free radicals
Polymer films
Free Radicals

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Koo, Min Ah ; Kim, Bong Jin ; Lee, Mi Hee ; Kwon, Byeong Ju ; Kim, Min Sung ; Seon, Gyeung Mi ; Kim, Dohyun ; Nam, Ki Chang ; Wang, Kang Kyun ; Kim, Yong Rok ; Park, Jong Chul. / Controlled Delivery of Extracellular ROS Based on Hematoporphyrin-Incorporated Polyurethane Film for Enhanced Proliferation of Endothelial Cells. In: ACS Applied Materials and Interfaces. 2016 ; Vol. 8, No. 42. pp. 28448-28457.
@article{300c7a6d21a2445bb5e83e041e939e4f,
title = "Controlled Delivery of Extracellular ROS Based on Hematoporphyrin-Incorporated Polyurethane Film for Enhanced Proliferation of Endothelial Cells",
abstract = "The principle of photodynamic treatment (PDT) involves the administration of photosensitizer (PS) at diseased tissues, followed by light irradiation to produce reactive oxygen species (ROS). In cells, a moderate increase in ROS plays an important role as signaling molecule to promote cell proliferation, whereas a severe increase of ROS causes cell damage. Previous studies have shown that low levels of ROS stimulate cell growth through PS drugs-treating PDT and nonthermal plasma treatment. However, these methods have side effects which are associated with low tissue selectivity and remaining of PS residues. To overcome such shortcomings, we designed hematoporphyrin-incorporated polyurethane (PU) film induced generation of extracellular ROS with singlet oxygen and free radicals. The film can easily control ROS production rate by regulating several parameters including light dose, PS dose. Also, its use facilitates targeted delivery of ROS to the specific lesion. Our study demonstrated that extracellular ROS could induce the formation of intracellular ROS. In vascular endothelial cells, a moderated increase in intracellular ROS also stimulated cell proliferation and cell cycle progression by accurate control of optimum levels of ROS with hematoporphyrin-incorporated polymer films. This modulation of cellular growth is expected to be an effective strategy for the design of next-generation PDT.",
author = "Koo, {Min Ah} and Kim, {Bong Jin} and Lee, {Mi Hee} and Kwon, {Byeong Ju} and Kim, {Min Sung} and Seon, {Gyeung Mi} and Dohyun Kim and Nam, {Ki Chang} and Wang, {Kang Kyun} and Kim, {Yong Rok} and Park, {Jong Chul}",
year = "2016",
month = "10",
day = "26",
doi = "10.1021/acsami.6b07628",
language = "English",
volume = "8",
pages = "28448--28457",
journal = "ACS applied materials & interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "42",

}

Controlled Delivery of Extracellular ROS Based on Hematoporphyrin-Incorporated Polyurethane Film for Enhanced Proliferation of Endothelial Cells. / Koo, Min Ah; Kim, Bong Jin; Lee, Mi Hee; Kwon, Byeong Ju; Kim, Min Sung; Seon, Gyeung Mi; Kim, Dohyun; Nam, Ki Chang; Wang, Kang Kyun; Kim, Yong Rok; Park, Jong Chul.

In: ACS Applied Materials and Interfaces, Vol. 8, No. 42, 26.10.2016, p. 28448-28457.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Controlled Delivery of Extracellular ROS Based on Hematoporphyrin-Incorporated Polyurethane Film for Enhanced Proliferation of Endothelial Cells

AU - Koo, Min Ah

AU - Kim, Bong Jin

AU - Lee, Mi Hee

AU - Kwon, Byeong Ju

AU - Kim, Min Sung

AU - Seon, Gyeung Mi

AU - Kim, Dohyun

AU - Nam, Ki Chang

AU - Wang, Kang Kyun

AU - Kim, Yong Rok

AU - Park, Jong Chul

PY - 2016/10/26

Y1 - 2016/10/26

N2 - The principle of photodynamic treatment (PDT) involves the administration of photosensitizer (PS) at diseased tissues, followed by light irradiation to produce reactive oxygen species (ROS). In cells, a moderate increase in ROS plays an important role as signaling molecule to promote cell proliferation, whereas a severe increase of ROS causes cell damage. Previous studies have shown that low levels of ROS stimulate cell growth through PS drugs-treating PDT and nonthermal plasma treatment. However, these methods have side effects which are associated with low tissue selectivity and remaining of PS residues. To overcome such shortcomings, we designed hematoporphyrin-incorporated polyurethane (PU) film induced generation of extracellular ROS with singlet oxygen and free radicals. The film can easily control ROS production rate by regulating several parameters including light dose, PS dose. Also, its use facilitates targeted delivery of ROS to the specific lesion. Our study demonstrated that extracellular ROS could induce the formation of intracellular ROS. In vascular endothelial cells, a moderated increase in intracellular ROS also stimulated cell proliferation and cell cycle progression by accurate control of optimum levels of ROS with hematoporphyrin-incorporated polymer films. This modulation of cellular growth is expected to be an effective strategy for the design of next-generation PDT.

AB - The principle of photodynamic treatment (PDT) involves the administration of photosensitizer (PS) at diseased tissues, followed by light irradiation to produce reactive oxygen species (ROS). In cells, a moderate increase in ROS plays an important role as signaling molecule to promote cell proliferation, whereas a severe increase of ROS causes cell damage. Previous studies have shown that low levels of ROS stimulate cell growth through PS drugs-treating PDT and nonthermal plasma treatment. However, these methods have side effects which are associated with low tissue selectivity and remaining of PS residues. To overcome such shortcomings, we designed hematoporphyrin-incorporated polyurethane (PU) film induced generation of extracellular ROS with singlet oxygen and free radicals. The film can easily control ROS production rate by regulating several parameters including light dose, PS dose. Also, its use facilitates targeted delivery of ROS to the specific lesion. Our study demonstrated that extracellular ROS could induce the formation of intracellular ROS. In vascular endothelial cells, a moderated increase in intracellular ROS also stimulated cell proliferation and cell cycle progression by accurate control of optimum levels of ROS with hematoporphyrin-incorporated polymer films. This modulation of cellular growth is expected to be an effective strategy for the design of next-generation PDT.

UR - http://www.scopus.com/inward/record.url?scp=84994029398&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994029398&partnerID=8YFLogxK

U2 - 10.1021/acsami.6b07628

DO - 10.1021/acsami.6b07628

M3 - Article

C2 - 27696825

AN - SCOPUS:84994029398

VL - 8

SP - 28448

EP - 28457

JO - ACS applied materials & interfaces

JF - ACS applied materials & interfaces

SN - 1944-8244

IS - 42

ER -