Controlled release of growth factors on allograft bone in vitro

Zhinong Huang, WonHyoung Ryu, Peigen Ren, Rainer Fasching, Stuart B. Goodman

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Allografts are important alternatives to autografts for treating defects after major bone loss. Bone growth factors have both local autocrine and paracrine effects and regulate the growth, proliferation, and differentiation of osteoprogenitor cells. To study the effects of prolonged, continuous, local delivery of growth factors on bone growth, we developed a new microelectromechanical system (MEMS) drug delivery device. Bone marrow cells from mice were seeded on mouse allograft discs and cultured in osteogenic media with osteogenic protein 1 (OP-1) and/or basic fibroblast growth factor (FGF-2) delivered from MEMS devices for 6 weeks. We monitored bone formation by changes of bone volume using micro-CT scanning and release of osteocalcin using ELISA. The data suggest the MEMS devices delivered constant concentrations of OP-1 and FGF-2 to the media. Bone marrow cells grew on the allografts and increased bone volume. Addition of OP-1 increased bone formation whereas FGF-2 decreased bone formation. Local delivery of growth factors over a prolonged period modulated the differentiation of osteoprogenitor cells on allograft bone.

Original languageEnglish
Pages (from-to)1905-1911
Number of pages7
JournalClinical Orthopaedics and Related Research
Volume466
Issue number8
DOIs
Publication statusPublished - 2008 Jan 1

Fingerprint

Fibroblast Growth Factor 2
Allografts
Bone Morphogenetic Protein 7
Intercellular Signaling Peptides and Proteins
Bone and Bones
Osteogenesis
Bone Development
Bone Marrow Cells
Equipment and Supplies
Cell Differentiation
Osteocalcin
Autografts
Drug Delivery Systems
Enzyme-Linked Immunosorbent Assay
In Vitro Techniques
Growth

All Science Journal Classification (ASJC) codes

  • Surgery
  • Orthopedics and Sports Medicine

Cite this

Huang, Zhinong ; Ryu, WonHyoung ; Ren, Peigen ; Fasching, Rainer ; Goodman, Stuart B. / Controlled release of growth factors on allograft bone in vitro. In: Clinical Orthopaedics and Related Research. 2008 ; Vol. 466, No. 8. pp. 1905-1911.
@article{a626a66c1b7d4efea3f26b74a9eefa93,
title = "Controlled release of growth factors on allograft bone in vitro",
abstract = "Allografts are important alternatives to autografts for treating defects after major bone loss. Bone growth factors have both local autocrine and paracrine effects and regulate the growth, proliferation, and differentiation of osteoprogenitor cells. To study the effects of prolonged, continuous, local delivery of growth factors on bone growth, we developed a new microelectromechanical system (MEMS) drug delivery device. Bone marrow cells from mice were seeded on mouse allograft discs and cultured in osteogenic media with osteogenic protein 1 (OP-1) and/or basic fibroblast growth factor (FGF-2) delivered from MEMS devices for 6 weeks. We monitored bone formation by changes of bone volume using micro-CT scanning and release of osteocalcin using ELISA. The data suggest the MEMS devices delivered constant concentrations of OP-1 and FGF-2 to the media. Bone marrow cells grew on the allografts and increased bone volume. Addition of OP-1 increased bone formation whereas FGF-2 decreased bone formation. Local delivery of growth factors over a prolonged period modulated the differentiation of osteoprogenitor cells on allograft bone.",
author = "Zhinong Huang and WonHyoung Ryu and Peigen Ren and Rainer Fasching and Goodman, {Stuart B.}",
year = "2008",
month = "1",
day = "1",
doi = "10.1007/s11999-008-0290-8",
language = "English",
volume = "466",
pages = "1905--1911",
journal = "Clinical Orthopaedics and Related Research",
issn = "0009-921X",
publisher = "Springer New York",
number = "8",

}

Controlled release of growth factors on allograft bone in vitro. / Huang, Zhinong; Ryu, WonHyoung; Ren, Peigen; Fasching, Rainer; Goodman, Stuart B.

In: Clinical Orthopaedics and Related Research, Vol. 466, No. 8, 01.01.2008, p. 1905-1911.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Controlled release of growth factors on allograft bone in vitro

AU - Huang, Zhinong

AU - Ryu, WonHyoung

AU - Ren, Peigen

AU - Fasching, Rainer

AU - Goodman, Stuart B.

PY - 2008/1/1

Y1 - 2008/1/1

N2 - Allografts are important alternatives to autografts for treating defects after major bone loss. Bone growth factors have both local autocrine and paracrine effects and regulate the growth, proliferation, and differentiation of osteoprogenitor cells. To study the effects of prolonged, continuous, local delivery of growth factors on bone growth, we developed a new microelectromechanical system (MEMS) drug delivery device. Bone marrow cells from mice were seeded on mouse allograft discs and cultured in osteogenic media with osteogenic protein 1 (OP-1) and/or basic fibroblast growth factor (FGF-2) delivered from MEMS devices for 6 weeks. We monitored bone formation by changes of bone volume using micro-CT scanning and release of osteocalcin using ELISA. The data suggest the MEMS devices delivered constant concentrations of OP-1 and FGF-2 to the media. Bone marrow cells grew on the allografts and increased bone volume. Addition of OP-1 increased bone formation whereas FGF-2 decreased bone formation. Local delivery of growth factors over a prolonged period modulated the differentiation of osteoprogenitor cells on allograft bone.

AB - Allografts are important alternatives to autografts for treating defects after major bone loss. Bone growth factors have both local autocrine and paracrine effects and regulate the growth, proliferation, and differentiation of osteoprogenitor cells. To study the effects of prolonged, continuous, local delivery of growth factors on bone growth, we developed a new microelectromechanical system (MEMS) drug delivery device. Bone marrow cells from mice were seeded on mouse allograft discs and cultured in osteogenic media with osteogenic protein 1 (OP-1) and/or basic fibroblast growth factor (FGF-2) delivered from MEMS devices for 6 weeks. We monitored bone formation by changes of bone volume using micro-CT scanning and release of osteocalcin using ELISA. The data suggest the MEMS devices delivered constant concentrations of OP-1 and FGF-2 to the media. Bone marrow cells grew on the allografts and increased bone volume. Addition of OP-1 increased bone formation whereas FGF-2 decreased bone formation. Local delivery of growth factors over a prolonged period modulated the differentiation of osteoprogenitor cells on allograft bone.

UR - http://www.scopus.com/inward/record.url?scp=48849096074&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=48849096074&partnerID=8YFLogxK

U2 - 10.1007/s11999-008-0290-8

DO - 10.1007/s11999-008-0290-8

M3 - Article

C2 - 18509711

AN - SCOPUS:48849096074

VL - 466

SP - 1905

EP - 1911

JO - Clinical Orthopaedics and Related Research

JF - Clinical Orthopaedics and Related Research

SN - 0009-921X

IS - 8

ER -