Conversion of glioma cells to glioma stem-like cells by angiocrine factors

Jun Kyum Kim, Hye Min Jeon, Hee Young Jeon, Se Yeong Oh, Eun Jung Kim, Xiong Jin, Se Hoon Kim, Sung Hak Kim, Xun Jin, Hyunggee Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Glioma stem-like cells (GSCs) contribute to tumor initiation, progression, and therapeutic resistance, but their cellular origin remains largely unknown. Here, using a stem/progenitor cell-fate tracking reporter system in which eGFP is expressed by promoter of OCT4 that is activated in stem/progenitor cells, we demonstrate that eGFP-negative glioma cells (GCs) became eGFP-positive-GCs in both in vitro cultures and in vivo xenografts. These eGFP-positive-GCs exhibited GSC features and primarily localized to the perivascular region in tumor xenografts, similar to the existence of OCT4-expressing GCs in the perivascular region of human glioblastoma specimens. Angiocrine factors, including nitric oxide (NO), converted eGFP-negative-GCs into eGFP-positive-GCs. Mechanistically, NO signaling conferred GSC features to GCs by increasing OCT4 and NOTCH signaling via ID4. NO signaling blockade and a suicide gene induction prevented tumorigenicity with a decrease in eGFP-positive-GCs in the perivascular region. Taken together, our results reveal the molecular mechanism underlying GSCs generation by cancer cell dedifferentiation.

Original languageEnglish
Pages (from-to)1013-1018
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume496
Issue number4
DOIs
Publication statusPublished - 2018 Feb 19

Fingerprint

Glioma
Nitric Oxide
Stem Cells
Stem cells
Heterografts
Tumors
Genes
Cells
Cell Dedifferentiation
Cell Tracking
Neoplasms
Glioblastoma
Suicide

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kim, J. K., Jeon, H. M., Jeon, H. Y., Oh, S. Y., Kim, E. J., Jin, X., ... Kim, H. (2018). Conversion of glioma cells to glioma stem-like cells by angiocrine factors. Biochemical and Biophysical Research Communications, 496(4), 1013-1018. https://doi.org/10.1016/j.bbrc.2017.02.076
Kim, Jun Kyum ; Jeon, Hye Min ; Jeon, Hee Young ; Oh, Se Yeong ; Kim, Eun Jung ; Jin, Xiong ; Kim, Se Hoon ; Kim, Sung Hak ; Jin, Xun ; Kim, Hyunggee. / Conversion of glioma cells to glioma stem-like cells by angiocrine factors. In: Biochemical and Biophysical Research Communications. 2018 ; Vol. 496, No. 4. pp. 1013-1018.
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Kim, JK, Jeon, HM, Jeon, HY, Oh, SY, Kim, EJ, Jin, X, Kim, SH, Kim, SH, Jin, X & Kim, H 2018, 'Conversion of glioma cells to glioma stem-like cells by angiocrine factors', Biochemical and Biophysical Research Communications, vol. 496, no. 4, pp. 1013-1018. https://doi.org/10.1016/j.bbrc.2017.02.076

Conversion of glioma cells to glioma stem-like cells by angiocrine factors. / Kim, Jun Kyum; Jeon, Hye Min; Jeon, Hee Young; Oh, Se Yeong; Kim, Eun Jung; Jin, Xiong; Kim, Se Hoon; Kim, Sung Hak; Jin, Xun; Kim, Hyunggee.

In: Biochemical and Biophysical Research Communications, Vol. 496, No. 4, 19.02.2018, p. 1013-1018.

Research output: Contribution to journalArticle

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AU - Jeon, Hye Min

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AU - Oh, Se Yeong

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AU - Jin, Xiong

AU - Kim, Se Hoon

AU - Kim, Sung Hak

AU - Jin, Xun

AU - Kim, Hyunggee

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