Jasmonic acid (JA) plays pivotal roles in diverse plant biological processes, including wound response. Chloroplast lipid hydrolysis is a critical step for JA biosynthesis, but the mechanism of this process remains elusive. We report here that DONGLE (DGL), a homolog of DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1), encodes a chloroplast-targeted lipase with strong galactolipase and weak phospholipase A1 activity. DGL is expressed in the leaves and has a specific role in maintaining basal JA content under normal conditions, and this expression regulates vegetative growth and is required for a rapid JA burst after wounding. During wounding, DGL and DAD1 have partially redundant functions for JA production, but they show different induction kinetics, indicating temporally separated roles: DGL plays a role in the early phase of JA production, and DAD1 plays a role in the late phase of JA production. Whereas DGL and DAD1 are necessary and sufficient for JA production, phospholipase D appears to modulate wound response by stimulating DGL and DAD1 expression.
Bibliographical noteFunding Information:
We thank X. Wang and J.G. Turner for kindly providing the pldα1 and cev1-1 seeds, R. Amasino for critical reading of the manuscript, and anonymous reviewers for critical comments. This work was supported partially by the Biogreen 21 Program (20050401-034-706-141-01-00); the Korea Ministry of Science and Technology under the National Research Laboratory Program (2006-01952); the Global Research Laboratory Program (2006-03870); and a grant from the Korea Science and Engineering Foundation through the Plant Metabolism Research Center, Kyung Hee University to I.L. S.-J.N. and H.K. were supported by MarineBio 21, the Ministry of Maritime Affairs & Fisheries, and Y.H., S.C., and J.Y. were supported by the Brain Korea 21 program.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Developmental Biology
- Cell Biology