Cooperation and Functional Diversification of Two Closely Related Galactolipase Genes for Jasmonate Biosynthesis

Youbong Hyun, Sungwook Choi, Hyun Ju Hwang, Jihyeon Yu, Sang Jip Nam, Jaeyoung Ko, Ju Young Park, Young Sam Seo, Eun Yu Kim, Stephen Beungtae Ryu, Woo Taek Kim, Yong Hwan Lee, Heonjoong Kang, Ilha Lee

Research output: Contribution to journalArticlepeer-review

116 Citations (Scopus)

Abstract

Jasmonic acid (JA) plays pivotal roles in diverse plant biological processes, including wound response. Chloroplast lipid hydrolysis is a critical step for JA biosynthesis, but the mechanism of this process remains elusive. We report here that DONGLE (DGL), a homolog of DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1), encodes a chloroplast-targeted lipase with strong galactolipase and weak phospholipase A1 activity. DGL is expressed in the leaves and has a specific role in maintaining basal JA content under normal conditions, and this expression regulates vegetative growth and is required for a rapid JA burst after wounding. During wounding, DGL and DAD1 have partially redundant functions for JA production, but they show different induction kinetics, indicating temporally separated roles: DGL plays a role in the early phase of JA production, and DAD1 plays a role in the late phase of JA production. Whereas DGL and DAD1 are necessary and sufficient for JA production, phospholipase D appears to modulate wound response by stimulating DGL and DAD1 expression.

Original languageEnglish
Pages (from-to)183-192
Number of pages10
JournalDevelopmental Cell
Volume14
Issue number2
DOIs
Publication statusPublished - 2008 Feb 12

Bibliographical note

Funding Information:
We thank X. Wang and J.G. Turner for kindly providing the pldα1 and cev1-1 seeds, R. Amasino for critical reading of the manuscript, and anonymous reviewers for critical comments. This work was supported partially by the Biogreen 21 Program (20050401-034-706-141-01-00); the Korea Ministry of Science and Technology under the National Research Laboratory Program (2006-01952); the Global Research Laboratory Program (2006-03870); and a grant from the Korea Science and Engineering Foundation through the Plant Metabolism Research Center, Kyung Hee University to I.L. S.-J.N. and H.K. were supported by MarineBio 21, the Ministry of Maritime Affairs & Fisheries, and Y.H., S.C., and J.Y. were supported by the Brain Korea 21 program.

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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