Coordination modes vs. antitumor activity: Synthesis and antitumor activity of novel platinum(II) complexes of N-substituted amino dicarboxylic acids

Yeong Sang Kim, Rita Song, Hyuncheol Chung, Moo Jin Jun, Youn Soo Sohn

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The trans-(±)-1,2-diaminocyclohexaneplatinum(II) complexes of multidentate L-glutamate (Glu) and L-aspartate (Asp) were prepared and their antitumor activity was examined in relation with their coordination modes. All these complexes were obtained as a mixture of (O,O′)- and (O,N)-chelate isomers due to rapid isomerization of the initially formed (O,O′)-isomer to the thermodynamically more stable (O,N)-isomer. The (O,O′)/(O,N)- isomeric mixture with the mole ratio of 80/20 exhibited excellent antitumor activity while the pure (O,N)-isomer was only marginally active. Therefore, in order to prevent the linkage isomerization of the active (O,O′)-isomer to the inactive (O,N)-isomer, we have designed N-substituted amino dicarboxylic acids as a leaving group and prepared a new series of complexes, [Pt(dach)(RGlu)] and [Pt(dach)(RAsp)] (dach=trans-(±)-1,2- diaminocyclohexane; R=acetyl (Ac), propionyl (Pro), pivaloyl (Piv), carbobenzyloxy (Cbz) or phthaloyl (Phth)) and characterized by means of elemental analyses, and 1 H NMR, 195 Pt NMR and IR spectroscopies. The N-substituted amino dicarboxylate ligands were found to coordinate to platinum(II) ion through only the (O,O′)-chelation mode, and their Pt(II) complexes were chemically stable in aqueous solution. The present Pt(II) complexes of N-substituted amino dicarboxylic acids showed excellent antitumor activity against both murine leukemia L1210 and human tumor cells. Especially, the highly hydrophobic N-phthaloylglutamate complex, [Pt(dach)(PhthGlu)], exhibited an outstanding in vitro activity (IC 50 =2.22 μM) on the human stomach cancer cells which are not responsive to cisplatin and carboplatin.

Original languageEnglish
Pages (from-to)98-104
Number of pages7
JournalJournal of Inorganic Biochemistry
Issue number1
Publication statusPublished - 2004 Jan 1


All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Inorganic Chemistry

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