COP1 E3 ligase protects HYL1 to retain microrna biogenesis

Seok Keun Cho, Samir Ben Chaabane, Pratik Shah, Christian Peter Poulsen, Seong Wook Yang

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Constitutive photomorphogenic 1 (COP1) is a RING-finger E3 ligase that plays a central role in photomorphogenesis by destabilizing many light-regulated transcription factors and photoreceptors. Here, we reveal a novel function for COP1 E3 ligase in controlling global miRNA biogenesis in Arabidopsis thaliana. In cop1 mutants, the level of miRNAs is dramatically reduced because of the diminution of HYPONASTIC LEAVES 1(HYL1), an RNA-binding protein required for precise miRNA processing. HYL1 is destabilized by an unidentified protease, which we tentatively call protease X, that specifically cleaves the N-terminal region from HYL1, thus neutralizing its function. Our results further show that the cytoplasmic partitioning of COP1 under light is essential to protect HYL1 against protease X. Taken together, we suggest a novel regulatory network involving HYL1, protease X, COP1 and light signalling that is indispensable for miRNA biogenesis in Arabidopsis thaliana.

Original languageEnglish
Article number6867
JournalNature communications
Volume5
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

biological evolution
protease
Ubiquitin-Protein Ligases
MicroRNAs
Peptide Hydrolases
Arabidopsis
Light
photoreceptors
RNA-Binding Proteins
Fingers
Transcription Factors
proteins
Processing

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Cho, Seok Keun ; Chaabane, Samir Ben ; Shah, Pratik ; Poulsen, Christian Peter ; Yang, Seong Wook. / COP1 E3 ligase protects HYL1 to retain microrna biogenesis. In: Nature communications. 2014 ; Vol. 5.
@article{eddf1722943d451ea55c64d8fd7c40d9,
title = "COP1 E3 ligase protects HYL1 to retain microrna biogenesis",
abstract = "Constitutive photomorphogenic 1 (COP1) is a RING-finger E3 ligase that plays a central role in photomorphogenesis by destabilizing many light-regulated transcription factors and photoreceptors. Here, we reveal a novel function for COP1 E3 ligase in controlling global miRNA biogenesis in Arabidopsis thaliana. In cop1 mutants, the level of miRNAs is dramatically reduced because of the diminution of HYPONASTIC LEAVES 1(HYL1), an RNA-binding protein required for precise miRNA processing. HYL1 is destabilized by an unidentified protease, which we tentatively call protease X, that specifically cleaves the N-terminal region from HYL1, thus neutralizing its function. Our results further show that the cytoplasmic partitioning of COP1 under light is essential to protect HYL1 against protease X. Taken together, we suggest a novel regulatory network involving HYL1, protease X, COP1 and light signalling that is indispensable for miRNA biogenesis in Arabidopsis thaliana.",
author = "Cho, {Seok Keun} and Chaabane, {Samir Ben} and Pratik Shah and Poulsen, {Christian Peter} and Yang, {Seong Wook}",
year = "2014",
month = "1",
day = "1",
doi = "10.1038/ncomms6867",
language = "English",
volume = "5",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

COP1 E3 ligase protects HYL1 to retain microrna biogenesis. / Cho, Seok Keun; Chaabane, Samir Ben; Shah, Pratik; Poulsen, Christian Peter; Yang, Seong Wook.

In: Nature communications, Vol. 5, 6867, 01.01.2014.

Research output: Contribution to journalArticle

TY - JOUR

T1 - COP1 E3 ligase protects HYL1 to retain microrna biogenesis

AU - Cho, Seok Keun

AU - Chaabane, Samir Ben

AU - Shah, Pratik

AU - Poulsen, Christian Peter

AU - Yang, Seong Wook

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Constitutive photomorphogenic 1 (COP1) is a RING-finger E3 ligase that plays a central role in photomorphogenesis by destabilizing many light-regulated transcription factors and photoreceptors. Here, we reveal a novel function for COP1 E3 ligase in controlling global miRNA biogenesis in Arabidopsis thaliana. In cop1 mutants, the level of miRNAs is dramatically reduced because of the diminution of HYPONASTIC LEAVES 1(HYL1), an RNA-binding protein required for precise miRNA processing. HYL1 is destabilized by an unidentified protease, which we tentatively call protease X, that specifically cleaves the N-terminal region from HYL1, thus neutralizing its function. Our results further show that the cytoplasmic partitioning of COP1 under light is essential to protect HYL1 against protease X. Taken together, we suggest a novel regulatory network involving HYL1, protease X, COP1 and light signalling that is indispensable for miRNA biogenesis in Arabidopsis thaliana.

AB - Constitutive photomorphogenic 1 (COP1) is a RING-finger E3 ligase that plays a central role in photomorphogenesis by destabilizing many light-regulated transcription factors and photoreceptors. Here, we reveal a novel function for COP1 E3 ligase in controlling global miRNA biogenesis in Arabidopsis thaliana. In cop1 mutants, the level of miRNAs is dramatically reduced because of the diminution of HYPONASTIC LEAVES 1(HYL1), an RNA-binding protein required for precise miRNA processing. HYL1 is destabilized by an unidentified protease, which we tentatively call protease X, that specifically cleaves the N-terminal region from HYL1, thus neutralizing its function. Our results further show that the cytoplasmic partitioning of COP1 under light is essential to protect HYL1 against protease X. Taken together, we suggest a novel regulatory network involving HYL1, protease X, COP1 and light signalling that is indispensable for miRNA biogenesis in Arabidopsis thaliana.

UR - http://www.scopus.com/inward/record.url?scp=84928972467&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928972467&partnerID=8YFLogxK

U2 - 10.1038/ncomms6867

DO - 10.1038/ncomms6867

M3 - Article

C2 - 25532508

AN - SCOPUS:84928972467

VL - 5

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 6867

ER -