Coping with polychlorinated biphenyl (PCB) toxicity: Physiological and genome-wide responses of Burkholderia xenovorans LB400 to PCB-mediated stress

J. Jacob Parnell, Joonhong Park, Vincent Denef, Tamara Tsoi, Syed Hashsham, John Quensen, James M. Tiedje

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The biodegradation of polychlorinated biphenyls (PCBs) relies on the ability of aerobic microorganisms such as Burkholderia xenovorans sp. LB400 to tolerate two potential modes of toxicity presented by PCB degradation: passive toxicity, as hydrophobic PCBs potentially disrupt membrane and protein function, and degradation-dependent toxicity from intermediates of incomplete degradation. We monitored the physiological characteristics and genome-wide expression patterns of LB400 in response to the presence of Aroclor 1242 (500 ppm) under low expression of the structural biphenyl pathway (succinate and benzoate growth) and under induction by biphenyl. We found no inhibition of growth or change in fatty acid profile due to PCBs under nondegrading conditions. Moreover, we observed no differential gene expression due to PCBs themselves. However, PCBs did have a slight effect on the biosurface area of LB400 cells and caused slight membrane separation. Upon activation of the biphenyl pathway, we found growth inhibition from PCBs beginning after exponential-phase growth suggestive of the accumulation of toxic compounds. Genome-wide expression profiling revealed 47 differentially expressed genes (0.56% of all genes) under these conditions. The biphenyl and catechol pathways were induced as expected, but the quinoprotein methanol metabolic pathway and a putative chloroacetaldehyde dehydrogenase were also highly expressed. As the latter protein is essential to conversion of toxic metabolites in dichloroethane degradation, it may play a similar role in the degradation of chlorinated aliphatic compounds resulting from PCB degradation.

Original languageEnglish
Pages (from-to)6607-6614
Number of pages8
JournalApplied and Environmental Microbiology
Volume72
Issue number10
DOIs
Publication statusPublished - 2006 Oct 1

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Burkholderia
Polychlorinated Biphenyls
polychlorinated biphenyls
PCB
genome
Genome
toxicity
biphenyl
degradation
Poisons
Growth
growth retardation
Ethylene Dichlorides
membrane
protein
catechol
gene
Benzoates
benzoates
Succinic Acid

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Parnell, J. Jacob ; Park, Joonhong ; Denef, Vincent ; Tsoi, Tamara ; Hashsham, Syed ; Quensen, John ; Tiedje, James M. / Coping with polychlorinated biphenyl (PCB) toxicity : Physiological and genome-wide responses of Burkholderia xenovorans LB400 to PCB-mediated stress. In: Applied and Environmental Microbiology. 2006 ; Vol. 72, No. 10. pp. 6607-6614.
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Coping with polychlorinated biphenyl (PCB) toxicity : Physiological and genome-wide responses of Burkholderia xenovorans LB400 to PCB-mediated stress. / Parnell, J. Jacob; Park, Joonhong; Denef, Vincent; Tsoi, Tamara; Hashsham, Syed; Quensen, John; Tiedje, James M.

In: Applied and Environmental Microbiology, Vol. 72, No. 10, 01.10.2006, p. 6607-6614.

Research output: Contribution to journalArticle

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T1 - Coping with polychlorinated biphenyl (PCB) toxicity

T2 - Physiological and genome-wide responses of Burkholderia xenovorans LB400 to PCB-mediated stress

AU - Parnell, J. Jacob

AU - Park, Joonhong

AU - Denef, Vincent

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AU - Tiedje, James M.

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