Corneal endothelial cytoskeletal changes in F-actin with aging, diabetes, and after cytochalasin exposure

Eungkweon Kim, D. H. Geroski, G. P. Holley, S. I. Urken, H. F. Edelhauser

Research output: Contribution to journalArticle

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Abstract

We investigated the changes in endothelial cytoskeletal F-actin that occur with aging, diabetes, and exposure to cytochalasin D. Rabbit corneas, human donor corneas (with or without polymegethism), and corneas of diabetic individuals were studied. Endothelial F-actin was stained using nitrobenzoxadiazole-phallacidin. Results of these experiments demonstrated that F-actin of the rabbit and human corneal endothelium was arranged in linear circumferential strands that formed a hexagonal array. After in vitro perfusion of cytochalasin D to the corneal endothelium, the F-actin became randomly distributed throughout the cytoplasm, the hexagonal shape of the endothelial cell was disrupted, and endothelial permeability to carboxyfluorescein increased. Changes in F-actin were also observed in the endothelium of the human corneas with polymegethism, and in donor tissue having had previous posterior chamber intraocular lens implantation. The corneas of diabetic individuals also showed marked irregular F-actin fibers crossing the endothelial cell cytoplasm. These abnormal patterns of F-actin may contribute in part to the polymegethism observed in the corneal endothelial cells and may be the result of constant stress in cell volume regulation, particularly in the corneas of diabetic individuals.

Original languageEnglish
Pages (from-to)329-335
Number of pages7
JournalAmerican Journal of Ophthalmology
Volume114
Issue number3
DOIs
Publication statusPublished - 1992 Jan 1

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Cytochalasins
Actins
Cornea
Cytochalasin D
Corneal Endothelium
Endothelial Cells
Cytoplasm
Rabbits
Intraocular Lens Implantation
Cell Size
Endothelium
Permeability
Perfusion
Tissue Donors

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Kim, Eungkweon ; Geroski, D. H. ; Holley, G. P. ; Urken, S. I. ; Edelhauser, H. F. / Corneal endothelial cytoskeletal changes in F-actin with aging, diabetes, and after cytochalasin exposure. In: American Journal of Ophthalmology. 1992 ; Vol. 114, No. 3. pp. 329-335.
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Corneal endothelial cytoskeletal changes in F-actin with aging, diabetes, and after cytochalasin exposure. / Kim, Eungkweon; Geroski, D. H.; Holley, G. P.; Urken, S. I.; Edelhauser, H. F.

In: American Journal of Ophthalmology, Vol. 114, No. 3, 01.01.1992, p. 329-335.

Research output: Contribution to journalArticle

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