Corneal endothelial damage by air bubbles during phacoemulsification

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Objective: To characterize the mechanism by which air bubbles damage the corneal endothelium during phacoemulsification. Materials and Methods: A series of experiments was conducted to expose the corneal endothelium of New Zealand white rabbit and human eyes that were obtained from an eye bank to air under different conditions. Phacoemulsification at different power settings and irrigation with and without the introduction of air into the anterior chamber were performed. Corneal endothelial perfusion experiments were conducted with air bubbles that were introduced into the perfusion chamber for 2 seconds to 1 hour. Air was also injected into the anterior chambers of anesthetized rabbits for 2 minutes to 3 hours. Corneas were stained with nitrobenzoxadiazole-phallacidin and examined with fluorescence microscopy. Selected corneas were also examined with scanning and transmission electron microscopy. Results: Intracameral air bubbles during phacoemulsification, irrigation, and perfusion studies resulted in a severe injury to the corneal endothelium in as little as 20 seconds. Intracameral air bubbles in a living rabbit resulted in a slower injury that was morphologically different from the more rapid injury. Conclusions: Air bubbles in intraocular fluids with a high surface tension can cause a ring- shaped pattern of damage to the corneal endothelium. The mechanism that caused this pattern of damage appears to be a surface tension phenomenon.

Original languageEnglish
Pages (from-to)81-88
Number of pages8
JournalArchives of Ophthalmology
Volume115
Issue number1
DOIs
Publication statusPublished - 1997 Jan 1

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Phacoemulsification
Air
Corneal Endothelium
Surface Tension
Perfusion
Anterior Chamber
Rabbits
Cornea
Wounds and Injuries
Eye Banks
Scanning Transmission Electron Microscopy
Corneal Injuries
Aqueous Humor
Fluorescence Microscopy

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

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Corneal endothelial damage by air bubbles during phacoemulsification. / Kim, Eungkweon.

In: Archives of Ophthalmology, Vol. 115, No. 1, 01.01.1997, p. 81-88.

Research output: Contribution to journalArticle

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