Correlation between G1 cyclins and HPV in the uterine cervix

Namhoon Cho, YoungTae Kim, Jae Wook Kim

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

G1 cyclin, as a candidate proto-oncogene, plays a key role as a cellular regulator through direct interaction with retinoblastoma gene product (pRB), p107, and cyclin-dependent kinase. Human papillomavirus (HPV) 16/18 E6/7 proteins may bind the unphosphorylated pRB and act as a down-regulator of cyclin D. However, this theory has not been proven and the relationship between cyclin E and HPV also remains unclear. Using formalin-fixed and paraffin-embedded cervical tissues, we examined for HPV types 16 and 18 by nested polymerase chain reaction (PCR), and for the accumulation of cyclin D1 and E by immunohistochemistry in 22 cases of normal cervix, 23 cases of cervical intraepithelial neoplasia (CIN), 39 cases of invasive squamous cell carcinoma (SCC), and 6 cases of adenocarcinoma. Cyclin index (CI) was defined as the percentage of positively labeled nuclei per 1000 cells. The accumulation of cyclin D1 was detected in the normal basal and parabasal epithelium and was markedly decreased in neoplastic epithelium (0.87% CI in CIN, 5.88% in SCC). However, cyclin E was absent in the normal cervix but increased in neoplastic epithelium (7.17% CI in CIN, 10.32% in SCC). Cyclin D1 expression was significantly low (p = 0.004), but cyclin E expression was significantly high (p = 0.026) in HPV-positive cases. Cyclin E plays a leading role in neoplastic transformation whereas cyclin D is down-regulated, especially when associated with HPV.

Original languageEnglish
Pages (from-to)339-347
Number of pages9
JournalInternational Journal of Gynecological Pathology
Volume16
Issue number4
DOIs
Publication statusPublished - 1997 Jan 1

Fingerprint

Cyclin G1
Cyclin E
Cervix Uteri
Cervical Intraepithelial Neoplasia
Cyclins
Cyclin D1
Cyclin D
Human papillomavirus 18
Squamous Cell Carcinoma
Human papillomavirus 16
Epithelium
Retinoblastoma Genes
Proto-Oncogenes
Cyclin-Dependent Kinases
Paraffin
Formaldehyde
Adenocarcinoma
Immunohistochemistry
Polymerase Chain Reaction
Proteins

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Obstetrics and Gynaecology

Cite this

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abstract = "G1 cyclin, as a candidate proto-oncogene, plays a key role as a cellular regulator through direct interaction with retinoblastoma gene product (pRB), p107, and cyclin-dependent kinase. Human papillomavirus (HPV) 16/18 E6/7 proteins may bind the unphosphorylated pRB and act as a down-regulator of cyclin D. However, this theory has not been proven and the relationship between cyclin E and HPV also remains unclear. Using formalin-fixed and paraffin-embedded cervical tissues, we examined for HPV types 16 and 18 by nested polymerase chain reaction (PCR), and for the accumulation of cyclin D1 and E by immunohistochemistry in 22 cases of normal cervix, 23 cases of cervical intraepithelial neoplasia (CIN), 39 cases of invasive squamous cell carcinoma (SCC), and 6 cases of adenocarcinoma. Cyclin index (CI) was defined as the percentage of positively labeled nuclei per 1000 cells. The accumulation of cyclin D1 was detected in the normal basal and parabasal epithelium and was markedly decreased in neoplastic epithelium (0.87{\%} CI in CIN, 5.88{\%} in SCC). However, cyclin E was absent in the normal cervix but increased in neoplastic epithelium (7.17{\%} CI in CIN, 10.32{\%} in SCC). Cyclin D1 expression was significantly low (p = 0.004), but cyclin E expression was significantly high (p = 0.026) in HPV-positive cases. Cyclin E plays a leading role in neoplastic transformation whereas cyclin D is down-regulated, especially when associated with HPV.",
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Correlation between G1 cyclins and HPV in the uterine cervix. / Cho, Namhoon; Kim, YoungTae; Kim, Jae Wook.

In: International Journal of Gynecological Pathology, Vol. 16, No. 4, 01.01.1997, p. 339-347.

Research output: Contribution to journalArticle

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