Corticotropin-releasing hormone downregulates IL-10 production by adaptive forkhead box protein 3-negative regulatory T cells in patients with atopic dermatitis

Sang Ho Oh, Chang Ook Park, Wen Hao Wu, Ji Young Kim, Shan Jin, Dashlkhumbe Byamba, Byung Gi Bae, Seongmin Noh, Beom Jin Lim, Ji Yeon Noh, Kwang Hoon Lee

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: Corticotropin-releasing hormone (CRH) is the central regulating hormone of the hypothalamic-pituitary-adrenal axis. CRH also has diverse functional effects in the periphery and is related to the aggravation of several cutaneous diseases; however, the effect of CRH on T cells in patients with atopic dermatitis (AD) has not been well evaluated. Objective: We investigated whether CRH directly affects peripheral TH1, TH2, and regulatory T (Treg) cells in patients with AD. Methods: We assessed whether T cells express the CRH receptor protein and mRNA by using flow cytometry, Western blotting, immunofluorescence, immunohistochemistry, and RT-PCR. We evaluated cytokine expression using ELISA after treating the T cells extracted from patients with AD and healthy control subjects (HCs) with CRH. Flow cytometry was then used to evaluate any direct effects of CRH on TH1, T H2, and Treg cells from patients with AD and HCs. Results: T cells from patients with AD expressed significantly lower CRH receptor 1/2 mRNA levels than T cells from HCs. T cells from HCs reacted with different IL-4 and IFN-γ secretions to CRH treatment, whereas T cells from patients with AD did not. IL-10 production was significantly decreased in the supernatants from both the HCs and patients with AD after CRH treatment. CRH upregulated IL-4 production by TH2 cells and downregulated IFN-γ production by TH1 cells in HCs. CRH also suppressed the production of IL-10 by forkhead box protein 3-negative Treg cells in both groups, but the difference was only significant in patients with AD. Conclusions: CRH-mediated suppression of IL-10 secretion from Treg cells might explain stress-related exacerbations in patients with AD.

Original languageEnglish
Pages (from-to)151-159.e6
JournalJournal of Allergy and Clinical Immunology
Volume129
Issue number1
DOIs
Publication statusPublished - 2012 Jan

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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