Coupling reactions of trehalose synthase from Pyrococcus horikoshii: Cost-effective synthesis and anti-adhesive activity of α-galactosyl oligosaccharides using a one-pot three-enzyme system with trehalose

Soo In Ryu, Jang Bin Woo, Soo Bok Lee

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6 Citations (Scopus)

Abstract

A new sugar nucleotide cycling (SNC) process was established in a one-pot three enzyme-coupled reaction using disaccharide trehalose. Trehalose synthase from Pyrococcus horikoshii could be applied to the SNC process for the synthesis of functional α-galactosyl oligosaccharides, α-galactose (Gal) epitopes and globotriose, using the effective regeneration of UDP-Gal. The α-Gal epitopes and globotriose were found to attach to the cell-surface of enteropathogenic Escherichia coli O127 (EPEC) which were bound to human Caco-2 cells. These α-galactosyl oligosaccharides were able to prohibit the attachment of EPEC, which could have resulted in colonization and disease. The α-Gal epitope III with a lactulose acceptor showed the most inhibitory activity of anti-adhesion. The results suggest that the α-galactosyl oligosaccharides may be alternative anti-adhesion molecules that overcome antibiotic resistance.

Original languageEnglish
Pages (from-to)743-746
Number of pages4
JournalBioresource technology
Volume136
DOIs
Publication statusPublished - 2013 May

Bibliographical note

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) Grant funded by the Ministry of Education, Science and Technology ( 2012R1A1A2005524 ). We also appreciate Korea Basic Research Institute for the contribution.

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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