Abstract
A methallylsilane coupling reagent, containing both a N-hydroxysuccinimidyl(NHS)-ester group and a UV/vis absorbing azobenzene linker undergoes acid-catalyzed immobilization on silica. Analysis of the UV/vis absorption band associated with the azobenzene group in the adduct enables facile quantitative determination of the extent of loading of the NHS groups. Reaction of NHS-groups on the silica surface with amine groups of GOx and rhodamine can be employed to generate enzyme or dye-immobilized silica for quantitative analysis.
Original language | English |
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Pages (from-to) | 2972-2975 |
Number of pages | 4 |
Journal | Organic Letters |
Volume | 20 |
Issue number | 10 |
DOIs | |
Publication status | Published - 2018 May 18 |
Bibliographical note
Funding Information:This work was supported by a grant from the National Research Foundation of Korea (NRF) (Grant 2016-R-1-A2b4009460).
All Science Journal Classification (ASJC) codes
- Biochemistry
- Physical and Theoretical Chemistry
- Organic Chemistry