A strategy to incorporate, physically constrain, and stabilize biologically active α-helical peptides into the polyacrylamide hydrogel network is developed. A bioactive RNA-binding helical peptide with polymerizable acryloyl groups at both ends of the peptide is synthesized and covalently captured in its bioactive helical conformation into the polyacrylamide network via radical polymerization. The usefulness of this approach is demonstrated by the specific recognition of RNAs in the polyacrylamide gel stabilization electrophoresis (PASE) system.
|Number of pages||4|
|Journal||Journal of Polymer Science, Part A: Polymer Chemistry|
|Publication status||Published - 2014 Mar 1|
All Science Journal Classification (ASJC) codes
- Polymers and Plastics
- Organic Chemistry
- Materials Chemistry