Covalent capture of α-helical peptides in polymer hydrogel network for polyacrylamide gel stabilization electrophoresis

Sanghun Han, Yong Beom Lim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A strategy to incorporate, physically constrain, and stabilize biologically active α-helical peptides into the polyacrylamide hydrogel network is developed. A bioactive RNA-binding helical peptide with polymerizable acryloyl groups at both ends of the peptide is synthesized and covalently captured in its bioactive helical conformation into the polyacrylamide network via radical polymerization. The usefulness of this approach is demonstrated by the specific recognition of RNAs in the polyacrylamide gel stabilization electrophoresis (PASE) system.

Original languageEnglish
Pages (from-to)596-599
Number of pages4
JournalJournal of Polymer Science, Part A: Polymer Chemistry
Volume52
Issue number5
DOIs
Publication statusPublished - 2014 Mar 1

Fingerprint

Hydrogel
Polyacrylates
Electrophoresis
Hydrogels
Peptides
Polymers
Gels
Stabilization
RNA
Free radical polymerization
Conformations
polyacrylamide gels

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

Cite this

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