CPR5-mediated nucleo-cytoplasmic localization of IAA12 and IAA19 controls lateral root development during abiotic stress

Heejae Nam, Soeun Han, Seungchul Lee, Hoyoung Nam, Hojun Lim, Garam Lee, Hyun Seob Cho, Tuong Vi Thi Dang, Sangkyu Choi, Myeong Min Lee, Ildoo Hwang

Research output: Contribution to journalArticlepeer-review


Plasticity of the root system architecture (RSA) is essential in enabling plants to cope with various environmental stresses and is mainly controlled by the phytohormone auxin. Lateral root development is a major determinant of RSA. Abiotic stresses reduce auxin signaling output, inhibiting lateral root development; however, how abiotic stress translates into a lower auxin signaling output is not fully understood. Here, we show that the nucleo-cytoplasmic distribution of the negative regulators of auxin signaling AUXIN/INDOLE-3-ACETIC ACID INDUCIBLE 12 (AUX/IAA12 or IAA12) and IAA19 determines lateral root development under various abiotic stress conditions. The cytoplasmic localization of IAA12 and IAA19 in the root elongation zone enforces auxin signaling output, allowing lateral root development. Among components of the nuclear pore complex, we show that CONSTITUTIVE EXPRESSOR OF PATHOGENESIS-RELATED GENES 5 (CPR5) selectively mediates the cytoplasmic translocation of IAA12/19. Under abiotic stress conditions, CPR5 expression is strongly decreased, resulting in the accumulation of nucleus-localized IAA12/19 in the root elongation zone and the suppression of lateral root development, which is reiterated in the cpr5 mutant. This study reveals a regulatory mechanism for auxin signaling whereby the spatial distribution of AUX/IAA regulators is critical for lateral root development, especially in fluctuating environmental conditions.

Original languageEnglish
Article numbere2209781120
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number3
Publication statusPublished - 2023 Jan 17

Bibliographical note

Funding Information:
ACKNOWLEDGMENTS. This work was supported by a grant from the New Breeding Technologies Development Program (project no. PJ016538), Rural Development Administration, Republic of Korea, and the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science and ICT (MIST) (project no. 2020R1A2C3012750). This research was supported by the BK21 FOUR funded by the Ministry of Education,Republic of Korea.We appreciate Dr. Aarti Gupta for editing the revised manuscript.

Publisher Copyright:
Copyright © 2023 the Author(s). Published by PNAS.

All Science Journal Classification (ASJC) codes

  • General


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