Critical Role of ATP-P2X7 Axis in UV-Induced Melanogenesis

Eun Jung Lee, Ji Young Kim, Yuri Ahn, Byeong min Lee, Yunkyung Heo, Shinwon Hwang, Si Hyung Lee, Jinu Lee, Gehoon Chung, SangHo Oh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purinergic signaling participates in skin physiology and pathology, such as hair growth, wound healing, inflammation, pain, and skin cancer. However, few studies have investigated the involvement of purinergic signaling in skin pigmentation. This study demonstrated that extracellular adenosine 5′-triphosphate (ATP) released from keratinocytes by UVB radiation promotes melanin production in primary human epidermal melanocytes and ex vivo skin cultures. Intracellular calcium ion and protein kinase C/CREB signaling contributed to ATP-mediated melanogenesis. Also, P2X7 receptor was proven to play a pivotal role in ATP-mediated melanogenesis because P2X7 receptor blockade abrogated ATP-induced melanin production. In addition, MNT1 cells with P2X7 receptor knockout using CRISPR/Cas9 system did not show any increase in MITF expression when co-cultured with UV-irradiated keratinocytes compared to MNT1 cells with intact P2X7 receptor, which showed increased expression of MITF. In conclusion, our results indicate that the extracellular ATP-P2X7 signaling axis is an adjunctive mechanism in UV-induced melanogenesis. Furthermore, ATP-induced purinergic signaling in melanocytes may alter skin pigmentation.

Original languageEnglish
Pages (from-to)1554-1563.e6
JournalJournal of Investigative Dermatology
Volume139
Issue number7
DOIs
Publication statusPublished - 2019 Jul 1

Fingerprint

Purinergic P2X7 Receptors
Adenosine
Adenosine Triphosphate
Skin
Skin Pigmentation
Melanocytes
Melanins
Keratinocytes
Skin Physiological Phenomena
Clustered Regularly Interspaced Short Palindromic Repeats
Physiology
Skin Neoplasms
Pathology
Wound Healing
Hair
Protein Kinase C
triphosphoric acid
Ions
Radiation
Inflammation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Lee, E. J., Kim, J. Y., Ahn, Y., Lee, B. M., Heo, Y., Hwang, S., ... Oh, S. (2019). Critical Role of ATP-P2X7 Axis in UV-Induced Melanogenesis. Journal of Investigative Dermatology, 139(7), 1554-1563.e6. https://doi.org/10.1016/j.jid.2019.02.031
Lee, Eun Jung ; Kim, Ji Young ; Ahn, Yuri ; Lee, Byeong min ; Heo, Yunkyung ; Hwang, Shinwon ; Lee, Si Hyung ; Lee, Jinu ; Chung, Gehoon ; Oh, SangHo. / Critical Role of ATP-P2X7 Axis in UV-Induced Melanogenesis. In: Journal of Investigative Dermatology. 2019 ; Vol. 139, No. 7. pp. 1554-1563.e6.
@article{4e925e83875d456a9d353772b4c04e93,
title = "Critical Role of ATP-P2X7 Axis in UV-Induced Melanogenesis",
abstract = "Purinergic signaling participates in skin physiology and pathology, such as hair growth, wound healing, inflammation, pain, and skin cancer. However, few studies have investigated the involvement of purinergic signaling in skin pigmentation. This study demonstrated that extracellular adenosine 5′-triphosphate (ATP) released from keratinocytes by UVB radiation promotes melanin production in primary human epidermal melanocytes and ex vivo skin cultures. Intracellular calcium ion and protein kinase C/CREB signaling contributed to ATP-mediated melanogenesis. Also, P2X7 receptor was proven to play a pivotal role in ATP-mediated melanogenesis because P2X7 receptor blockade abrogated ATP-induced melanin production. In addition, MNT1 cells with P2X7 receptor knockout using CRISPR/Cas9 system did not show any increase in MITF expression when co-cultured with UV-irradiated keratinocytes compared to MNT1 cells with intact P2X7 receptor, which showed increased expression of MITF. In conclusion, our results indicate that the extracellular ATP-P2X7 signaling axis is an adjunctive mechanism in UV-induced melanogenesis. Furthermore, ATP-induced purinergic signaling in melanocytes may alter skin pigmentation.",
author = "Lee, {Eun Jung} and Kim, {Ji Young} and Yuri Ahn and Lee, {Byeong min} and Yunkyung Heo and Shinwon Hwang and Lee, {Si Hyung} and Jinu Lee and Gehoon Chung and SangHo Oh",
year = "2019",
month = "7",
day = "1",
doi = "10.1016/j.jid.2019.02.031",
language = "English",
volume = "139",
pages = "1554--1563.e6",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "7",

}

Lee, EJ, Kim, JY, Ahn, Y, Lee, BM, Heo, Y, Hwang, S, Lee, SH, Lee, J, Chung, G & Oh, S 2019, 'Critical Role of ATP-P2X7 Axis in UV-Induced Melanogenesis', Journal of Investigative Dermatology, vol. 139, no. 7, pp. 1554-1563.e6. https://doi.org/10.1016/j.jid.2019.02.031

Critical Role of ATP-P2X7 Axis in UV-Induced Melanogenesis. / Lee, Eun Jung; Kim, Ji Young; Ahn, Yuri; Lee, Byeong min; Heo, Yunkyung; Hwang, Shinwon; Lee, Si Hyung; Lee, Jinu; Chung, Gehoon; Oh, SangHo.

In: Journal of Investigative Dermatology, Vol. 139, No. 7, 01.07.2019, p. 1554-1563.e6.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Critical Role of ATP-P2X7 Axis in UV-Induced Melanogenesis

AU - Lee, Eun Jung

AU - Kim, Ji Young

AU - Ahn, Yuri

AU - Lee, Byeong min

AU - Heo, Yunkyung

AU - Hwang, Shinwon

AU - Lee, Si Hyung

AU - Lee, Jinu

AU - Chung, Gehoon

AU - Oh, SangHo

PY - 2019/7/1

Y1 - 2019/7/1

N2 - Purinergic signaling participates in skin physiology and pathology, such as hair growth, wound healing, inflammation, pain, and skin cancer. However, few studies have investigated the involvement of purinergic signaling in skin pigmentation. This study demonstrated that extracellular adenosine 5′-triphosphate (ATP) released from keratinocytes by UVB radiation promotes melanin production in primary human epidermal melanocytes and ex vivo skin cultures. Intracellular calcium ion and protein kinase C/CREB signaling contributed to ATP-mediated melanogenesis. Also, P2X7 receptor was proven to play a pivotal role in ATP-mediated melanogenesis because P2X7 receptor blockade abrogated ATP-induced melanin production. In addition, MNT1 cells with P2X7 receptor knockout using CRISPR/Cas9 system did not show any increase in MITF expression when co-cultured with UV-irradiated keratinocytes compared to MNT1 cells with intact P2X7 receptor, which showed increased expression of MITF. In conclusion, our results indicate that the extracellular ATP-P2X7 signaling axis is an adjunctive mechanism in UV-induced melanogenesis. Furthermore, ATP-induced purinergic signaling in melanocytes may alter skin pigmentation.

AB - Purinergic signaling participates in skin physiology and pathology, such as hair growth, wound healing, inflammation, pain, and skin cancer. However, few studies have investigated the involvement of purinergic signaling in skin pigmentation. This study demonstrated that extracellular adenosine 5′-triphosphate (ATP) released from keratinocytes by UVB radiation promotes melanin production in primary human epidermal melanocytes and ex vivo skin cultures. Intracellular calcium ion and protein kinase C/CREB signaling contributed to ATP-mediated melanogenesis. Also, P2X7 receptor was proven to play a pivotal role in ATP-mediated melanogenesis because P2X7 receptor blockade abrogated ATP-induced melanin production. In addition, MNT1 cells with P2X7 receptor knockout using CRISPR/Cas9 system did not show any increase in MITF expression when co-cultured with UV-irradiated keratinocytes compared to MNT1 cells with intact P2X7 receptor, which showed increased expression of MITF. In conclusion, our results indicate that the extracellular ATP-P2X7 signaling axis is an adjunctive mechanism in UV-induced melanogenesis. Furthermore, ATP-induced purinergic signaling in melanocytes may alter skin pigmentation.

UR - http://www.scopus.com/inward/record.url?scp=85066074379&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85066074379&partnerID=8YFLogxK

U2 - 10.1016/j.jid.2019.02.031

DO - 10.1016/j.jid.2019.02.031

M3 - Article

VL - 139

SP - 1554-1563.e6

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 7

ER -