Optical imaging modalities have been utilized as important tools to evaluate skin lesions. Accurate analysis of hyper-pigmentation is important in the evaluation of sun damage, inflammation, and other skin disorders. In this study, both cross-polarization and fluorescent color images were obtained at identical facial skin region and hyper-pigmentation regions were extracted by applying a series of image processing. Cross-polarization image provides subsurface skin information without specular reflection and fluorescence image emphasizes pigmentation in epidermal region. The results were quantitatively evaluated and compared to investigate the difference between two imaging modalities in the evaluation of hyper-pigmentation regions.