Cross-reactivity between group-5 and-21 mite allergens from Dermatophagoides farinae, Tyrophagus putrescentiae and Blomia tropicalis

Chung Ryul Kim, Kyoung Yong Jeong, Myung Hee Yi, Hyoung Pyo Kim, Ho Joon Shin, Tai Soon Yong

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Group-5 and group-21 allergens, produced by house dust mites and storage mites are 36.6-55.8% identical in their sequences and are recognized by at least 50% of immunoglobulin (Ig)E from the sera of individuals allergic to dust mites. In the present study, recombinant group-5 and-21 allergens from three mite species, Dermatophagoides farinae (rDer f 5 and 21), Tyrophagus putrescentiae (rTyr p 5 and 21), and Blomia tropicalis (rBlo t 5 and 21), were purified from Escherichia coli, and the IgE reactivities and cross-reactivities of these allergen variants were assessed. The IgE binding frequencies of rDer f 5, rDer f 21, rTyr p 5, rTyr p 21, rBlo t and rBlo t 21 proteins were 64.95, 65.98, 30.41, 41.24, 30.93 and 21.65%, respectively. The IgE reactivity of rDer f 5 correlated highly with that of rDer f 21 (r=0.733). rTyr p 5 exhibited the highest level of correlation with rTyr p 21 (r=0.950), while the correlation of rBlo t 5 with rBlo t 21 was the lowest observed (r=0.104). The binding of IgE to rDer f 5 and rDer f 21 was not inhibited by any allergens but themselves. While rDer f 5 inhibited only 60.3% of IgE binding to rBlo t 5, rDer f 21 exhibited a high inhibitory effect against rTyr p 5 (93.01%), rTyr p 21 (92.12%), rBlo t 5 (86.97%) and rBlo t 21 (70.30%), implying cross-reactivity among mite species. The results of the present study demonstrated that the majority of the IgE reactivity to group-5 and-21 storage mite allergens is due to cross-reaction. It is therefore imperative to develop an accurate, component-resolved diagnosis for dust mite allergies.

Original languageEnglish
Pages (from-to)5467-5474
Number of pages8
JournalMolecular Medicine Reports
Volume12
Issue number4
DOIs
Publication statusPublished - 2015 Oct 1

Fingerprint

Dermatophagoides Antigens
Mites
Immunoglobulin E
Allergens
Acaridae
Dust
Dermatophagoides farinae
Allergies
Cross Reactions
Escherichia coli
Hypersensitivity
Serum

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Oncology
  • Cancer Research

Cite this

Kim, Chung Ryul ; Jeong, Kyoung Yong ; Yi, Myung Hee ; Kim, Hyoung Pyo ; Shin, Ho Joon ; Yong, Tai Soon. / Cross-reactivity between group-5 and-21 mite allergens from Dermatophagoides farinae, Tyrophagus putrescentiae and Blomia tropicalis. In: Molecular Medicine Reports. 2015 ; Vol. 12, No. 4. pp. 5467-5474.
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title = "Cross-reactivity between group-5 and-21 mite allergens from Dermatophagoides farinae, Tyrophagus putrescentiae and Blomia tropicalis",
abstract = "Group-5 and group-21 allergens, produced by house dust mites and storage mites are 36.6-55.8{\%} identical in their sequences and are recognized by at least 50{\%} of immunoglobulin (Ig)E from the sera of individuals allergic to dust mites. In the present study, recombinant group-5 and-21 allergens from three mite species, Dermatophagoides farinae (rDer f 5 and 21), Tyrophagus putrescentiae (rTyr p 5 and 21), and Blomia tropicalis (rBlo t 5 and 21), were purified from Escherichia coli, and the IgE reactivities and cross-reactivities of these allergen variants were assessed. The IgE binding frequencies of rDer f 5, rDer f 21, rTyr p 5, rTyr p 21, rBlo t and rBlo t 21 proteins were 64.95, 65.98, 30.41, 41.24, 30.93 and 21.65{\%}, respectively. The IgE reactivity of rDer f 5 correlated highly with that of rDer f 21 (r=0.733). rTyr p 5 exhibited the highest level of correlation with rTyr p 21 (r=0.950), while the correlation of rBlo t 5 with rBlo t 21 was the lowest observed (r=0.104). The binding of IgE to rDer f 5 and rDer f 21 was not inhibited by any allergens but themselves. While rDer f 5 inhibited only 60.3{\%} of IgE binding to rBlo t 5, rDer f 21 exhibited a high inhibitory effect against rTyr p 5 (93.01{\%}), rTyr p 21 (92.12{\%}), rBlo t 5 (86.97{\%}) and rBlo t 21 (70.30{\%}), implying cross-reactivity among mite species. The results of the present study demonstrated that the majority of the IgE reactivity to group-5 and-21 storage mite allergens is due to cross-reaction. It is therefore imperative to develop an accurate, component-resolved diagnosis for dust mite allergies.",
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Cross-reactivity between group-5 and-21 mite allergens from Dermatophagoides farinae, Tyrophagus putrescentiae and Blomia tropicalis. / Kim, Chung Ryul; Jeong, Kyoung Yong; Yi, Myung Hee; Kim, Hyoung Pyo; Shin, Ho Joon; Yong, Tai Soon.

In: Molecular Medicine Reports, Vol. 12, No. 4, 01.10.2015, p. 5467-5474.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cross-reactivity between group-5 and-21 mite allergens from Dermatophagoides farinae, Tyrophagus putrescentiae and Blomia tropicalis

AU - Kim, Chung Ryul

AU - Jeong, Kyoung Yong

AU - Yi, Myung Hee

AU - Kim, Hyoung Pyo

AU - Shin, Ho Joon

AU - Yong, Tai Soon

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AB - Group-5 and group-21 allergens, produced by house dust mites and storage mites are 36.6-55.8% identical in their sequences and are recognized by at least 50% of immunoglobulin (Ig)E from the sera of individuals allergic to dust mites. In the present study, recombinant group-5 and-21 allergens from three mite species, Dermatophagoides farinae (rDer f 5 and 21), Tyrophagus putrescentiae (rTyr p 5 and 21), and Blomia tropicalis (rBlo t 5 and 21), were purified from Escherichia coli, and the IgE reactivities and cross-reactivities of these allergen variants were assessed. The IgE binding frequencies of rDer f 5, rDer f 21, rTyr p 5, rTyr p 21, rBlo t and rBlo t 21 proteins were 64.95, 65.98, 30.41, 41.24, 30.93 and 21.65%, respectively. The IgE reactivity of rDer f 5 correlated highly with that of rDer f 21 (r=0.733). rTyr p 5 exhibited the highest level of correlation with rTyr p 21 (r=0.950), while the correlation of rBlo t 5 with rBlo t 21 was the lowest observed (r=0.104). The binding of IgE to rDer f 5 and rDer f 21 was not inhibited by any allergens but themselves. While rDer f 5 inhibited only 60.3% of IgE binding to rBlo t 5, rDer f 21 exhibited a high inhibitory effect against rTyr p 5 (93.01%), rTyr p 21 (92.12%), rBlo t 5 (86.97%) and rBlo t 21 (70.30%), implying cross-reactivity among mite species. The results of the present study demonstrated that the majority of the IgE reactivity to group-5 and-21 storage mite allergens is due to cross-reaction. It is therefore imperative to develop an accurate, component-resolved diagnosis for dust mite allergies.

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