Crosstalk between platelet-derived growth factor-induced Nox4 activation and MUC8 gene overexpression in human airway epithelial cells

Hyun Jik Kim, Chang-Hoon Kim, Ji Hwan Ryu, Jung Hee Joo, Sang Nam Lee, Min Ji Kim, Jeung Gweon Lee, Yun Soo Bae, Joo Heon Yoon

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) contribute to chronic airway inflammation, and NADPH oxidase (Nox) is an important source of ROS. However, little is known of the role that ROS play in chronic upper respiratory tract inflammation. We investigated the mechanism of ROS generation and its association with mucin gene overexpression in the nasal epithelium. The level of platelet-derived growth factor (PDGF) expression was increased in sinusitis mucosa, and high-level PDGF expression induced intracellular ROS, followed by MUC8 gene overexpression in normal human nasal epithelial cells. Knockdown of Nox4 expression with Nox4 siRNA decreased PDGF-induced intracellular ROS and MUC8 expression. Infection with an adenovirus containing Nox4 cDNA resulted in Nox4 overexpression and increased intracellular levels of ROS and MUC8 expression. PDGF and Nox4 overexpression are essential components of intracellular ROS generation and may contribute to chronic inflammation in the nasal epithelium through induction of MUC8 overexpression.

Original languageEnglish
Pages (from-to)1039-1052
Number of pages14
JournalFree Radical Biology and Medicine
Volume50
Issue number9
DOIs
Publication statusPublished - 2011 May 1

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Platelet-Derived Growth Factor
Crosstalk
Transcriptional Activation
Reactive Oxygen Species
Genes
Epithelial Cells
Chemical activation
Nasal Mucosa
Inflammation
Adenoviridae Infections
NADPH Oxidase
Sinusitis
Mucins
Nose
Respiratory System
Small Interfering RNA
Mucous Membrane
Complementary DNA
Association reactions

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology (medical)

Cite this

Kim, Hyun Jik ; Kim, Chang-Hoon ; Ryu, Ji Hwan ; Joo, Jung Hee ; Lee, Sang Nam ; Kim, Min Ji ; Lee, Jeung Gweon ; Bae, Yun Soo ; Yoon, Joo Heon. / Crosstalk between platelet-derived growth factor-induced Nox4 activation and MUC8 gene overexpression in human airway epithelial cells. In: Free Radical Biology and Medicine. 2011 ; Vol. 50, No. 9. pp. 1039-1052.
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Crosstalk between platelet-derived growth factor-induced Nox4 activation and MUC8 gene overexpression in human airway epithelial cells. / Kim, Hyun Jik; Kim, Chang-Hoon; Ryu, Ji Hwan; Joo, Jung Hee; Lee, Sang Nam; Kim, Min Ji; Lee, Jeung Gweon; Bae, Yun Soo; Yoon, Joo Heon.

In: Free Radical Biology and Medicine, Vol. 50, No. 9, 01.05.2011, p. 1039-1052.

Research output: Contribution to journalArticle

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