Crosstalk between platelet-derived growth factor-induced Nox4 activation and MUC8 gene overexpression in human airway epithelial cells

Hyun Jik Kim, Chang Hoon Kim, Ji Hwan Ryu, Jung Hee Joo, Sang Nam Lee, Min Ji Kim, Jeung Gweon Lee, Yun Soo Bae, Joo Heon Yoon

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18 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) contribute to chronic airway inflammation, and NADPH oxidase (Nox) is an important source of ROS. However, little is known of the role that ROS play in chronic upper respiratory tract inflammation. We investigated the mechanism of ROS generation and its association with mucin gene overexpression in the nasal epithelium. The level of platelet-derived growth factor (PDGF) expression was increased in sinusitis mucosa, and high-level PDGF expression induced intracellular ROS, followed by MUC8 gene overexpression in normal human nasal epithelial cells. Knockdown of Nox4 expression with Nox4 siRNA decreased PDGF-induced intracellular ROS and MUC8 expression. Infection with an adenovirus containing Nox4 cDNA resulted in Nox4 overexpression and increased intracellular levels of ROS and MUC8 expression. PDGF and Nox4 overexpression are essential components of intracellular ROS generation and may contribute to chronic inflammation in the nasal epithelium through induction of MUC8 overexpression.

Original languageEnglish
Pages (from-to)1039-1052
Number of pages14
JournalFree Radical Biology and Medicine
Volume50
Issue number9
DOIs
Publication statusPublished - 2011 May 1

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology (medical)

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