Cryptococcal Titan Cell Formation Is Regulated by G-Protein Signaling in Response to Multiple Stimuli

Laura H. Okagaki, Yina Wang, Elizabeth R. Ballou, Teresa R. O'Meara, Yong-Sun Bahn, J. Andrew Alspaugh, Chaoyang Xue, Kirsten Nielsen

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The titan cell is a recently described morphological form of the pathogenic fungus Cryptococcus neoformans. Occurring during the earliest stages of lung infection, titan cells are 5 to 10 times larger than the normal yeast-like cells, thereby resisting engulfment by lung phagocytes and favoring the persistence of infection. These enlarged cells exhibit an altered capsule structure, a thickened cell wall, increased ploidy, and resistance to nitrosative and oxidative stresses. We demonstrate that two G-protein-coupled receptors are important for induction of the titan cell phenotype: the Ste3a pheromone receptor (in mating type a cells) and the Gpr5 protein. Both receptors control titan cell formation through elements of the cyclic AMP (cAMP)/protein kinase A (PKA) pathway. This conserved signaling pathway, in turn, mediates its effect on titan cells through the PKA-regulated Rim101 transcription factor. Additional downstream effectors required for titan cell formation include the G 1 cyclin Pcl103, the Rho104 GTPase, and two GTPase-activating proteins, Gap1 and Cnc1560. These observations support developing models in which the PKA signaling pathway coordinately regulates many virulence-associated phenotypes in diverse human pathogens.

Original languageEnglish
Pages (from-to)1306-1316
Number of pages11
JournalEukaryotic Cell
Volume10
Issue number10
DOIs
Publication statusPublished - 2011 Oct 1

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Saturn
GTP-Binding Proteins
Cyclic AMP-Dependent Protein Kinases
Cyclin G
Pheromone Receptors
Phenotype
GTPase-Activating Proteins
Adenylate Kinase
Lung
Cryptococcus neoformans
Ploidies
GTP Phosphohydrolases
Phagocytes
G-Protein-Coupled Receptors
Infection
Cyclic AMP
Cell Wall
Capsules
Virulence
Oxidative Stress

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Okagaki, L. H., Wang, Y., Ballou, E. R., O'Meara, T. R., Bahn, Y-S., Alspaugh, J. A., ... Nielsen, K. (2011). Cryptococcal Titan Cell Formation Is Regulated by G-Protein Signaling in Response to Multiple Stimuli Eukaryotic Cell, 10(10), 1306-1316. https://doi.org/10.1128/EC.05179-11
Okagaki, Laura H. ; Wang, Yina ; Ballou, Elizabeth R. ; O'Meara, Teresa R. ; Bahn, Yong-Sun ; Alspaugh, J. Andrew ; Xue, Chaoyang ; Nielsen, Kirsten. / Cryptococcal Titan Cell Formation Is Regulated by G-Protein Signaling in Response to Multiple Stimuli In: Eukaryotic Cell. 2011 ; Vol. 10, No. 10. pp. 1306-1316.
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Okagaki, LH, Wang, Y, Ballou, ER, O'Meara, TR, Bahn, Y-S, Alspaugh, JA, Xue, C & Nielsen, K 2011, 'Cryptococcal Titan Cell Formation Is Regulated by G-Protein Signaling in Response to Multiple Stimuli ', Eukaryotic Cell, vol. 10, no. 10, pp. 1306-1316. https://doi.org/10.1128/EC.05179-11

Cryptococcal Titan Cell Formation Is Regulated by G-Protein Signaling in Response to Multiple Stimuli . / Okagaki, Laura H.; Wang, Yina; Ballou, Elizabeth R.; O'Meara, Teresa R.; Bahn, Yong-Sun; Alspaugh, J. Andrew; Xue, Chaoyang; Nielsen, Kirsten.

In: Eukaryotic Cell, Vol. 10, No. 10, 01.10.2011, p. 1306-1316.

Research output: Contribution to journalArticle

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