Crystal structure and functional characterization of a light-driven chloride pump having an NTQ motif

Kuglae Kim, Soon Kyeong Kwon, Sung Hoon Jun, Jeong Seok Cha, Hoyoung Kim, Weontae Lee, Jihyun F. Kim, Hyun Soo Cho

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A novel light-driven chloride-pumping rhodopsin (ClR) containing an NTQ motif' in its putative ion conduction pathway has been discovered and functionally characterized in a genomic analysis study of a marine bacterium. Here we report the crystal structure of ClR from the flavobacterium Nonlabens marinus S1-08 T determined under two conditions at 2.0 and 1.56 Å resolutions. The structures reveal two chloride-binding sites, one around the protonated Schiff base and the other on a cytoplasmic loop. We identify a 3 omega motif' formed by three non-consecutive aromatic amino acids that is correlated with the B-C loop orientation. Detailed ClR structural analyses with functional studies in E. coli reveal the chloride ion transduction pathway. Our results help understand the molecular mechanism and physiological role of ClR and provide a structural basis for optogenetic applications.

Original languageEnglish
Article number12677
JournalNature communications
Volume7
DOIs
Publication statusPublished - 2016 Aug 24

Fingerprint

Chlorides
Rhodopsin
Crystal structure
chlorides
Pumps
pumps
Light
crystal structure
pumping
Optogenetics
Ions
Flavobacterium
Aromatic Amino Acids
Schiff Bases
Escherichia coli
bacteria
imines
amino acids
Bacteria
ions

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

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title = "Crystal structure and functional characterization of a light-driven chloride pump having an NTQ motif",
abstract = "A novel light-driven chloride-pumping rhodopsin (ClR) containing an NTQ motif' in its putative ion conduction pathway has been discovered and functionally characterized in a genomic analysis study of a marine bacterium. Here we report the crystal structure of ClR from the flavobacterium Nonlabens marinus S1-08 T determined under two conditions at 2.0 and 1.56 {\AA} resolutions. The structures reveal two chloride-binding sites, one around the protonated Schiff base and the other on a cytoplasmic loop. We identify a 3 omega motif' formed by three non-consecutive aromatic amino acids that is correlated with the B-C loop orientation. Detailed ClR structural analyses with functional studies in E. coli reveal the chloride ion transduction pathway. Our results help understand the molecular mechanism and physiological role of ClR and provide a structural basis for optogenetic applications.",
author = "Kuglae Kim and Kwon, {Soon Kyeong} and Jun, {Sung Hoon} and Cha, {Jeong Seok} and Hoyoung Kim and Weontae Lee and Kim, {Jihyun F.} and Cho, {Hyun Soo}",
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Crystal structure and functional characterization of a light-driven chloride pump having an NTQ motif. / Kim, Kuglae; Kwon, Soon Kyeong; Jun, Sung Hoon; Cha, Jeong Seok; Kim, Hoyoung; Lee, Weontae; Kim, Jihyun F.; Cho, Hyun Soo.

In: Nature communications, Vol. 7, 12677, 24.08.2016.

Research output: Contribution to journalArticle

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AU - Kim, Kuglae

AU - Kwon, Soon Kyeong

AU - Jun, Sung Hoon

AU - Cha, Jeong Seok

AU - Kim, Hoyoung

AU - Lee, Weontae

AU - Kim, Jihyun F.

AU - Cho, Hyun Soo

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