Crystal structure and functional insight of HP0420-homolog from Helicobacter felis

Shunfu Piao, Xiao Ling Jin, Bo Young Yun, Nahee Kim, Hyun Soo Cho, Minoru Fukuda, Heeseob Lee, Nam Chul Ha

Research output: Contribution to journalArticle

Abstract

Helicobacter pylori infect more than half of the world's population and are considered a cause of peptic ulcer disease and gastric cancer. Recently, hypothetical gene HP0421 was identified in H. pylori as a cholesterol α-glucosyltransferase, which is required to synthesize cholesteryl glucosides, essential cell wall components of the bacteria. In the same gene-cluster, HP0420 was co-identified, whose function remains unknown. Here we report the crystal structure of HP0420-homolog of H. felis (HF0420) to gain insight into the function of HP0420. The crystal structure, combined with size-exclusion chromatography, reveals that HF0420 adopts a homodimeric hot-dog fold. The crystal structure suggests that HF0420 has enzymatic activity that involves a conserved histidine residue at the end of the central α-helix. Subsequent biochemical studies provide clues to the function of HP0420 and HF0420.

Original languageEnglish
Pages (from-to)940-946
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume394
Issue number4
DOIs
Publication statusPublished - 2010 Apr 16

Fingerprint

Helicobacter felis
Helicobacter pylori
Crystal structure
Glucosyltransferases
Cellular Structures
Multigene Family
Genes
Peptic Ulcer
Histidine
Cell Wall
Stomach Neoplasms
Gel Chromatography
Size exclusion chromatography
Cholesterol
Dogs
Bacteria
Cells
Population

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Piao, Shunfu ; Jin, Xiao Ling ; Yun, Bo Young ; Kim, Nahee ; Cho, Hyun Soo ; Fukuda, Minoru ; Lee, Heeseob ; Ha, Nam Chul. / Crystal structure and functional insight of HP0420-homolog from Helicobacter felis. In: Biochemical and Biophysical Research Communications. 2010 ; Vol. 394, No. 4. pp. 940-946.
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Crystal structure and functional insight of HP0420-homolog from Helicobacter felis. / Piao, Shunfu; Jin, Xiao Ling; Yun, Bo Young; Kim, Nahee; Cho, Hyun Soo; Fukuda, Minoru; Lee, Heeseob; Ha, Nam Chul.

In: Biochemical and Biophysical Research Communications, Vol. 394, No. 4, 16.04.2010, p. 940-946.

Research output: Contribution to journalArticle

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