Crystal structure of Fushi tarazu factor 1 ligand binding domain/Fushi tarazu peptide complex identifies new class of nuclear receptors

Jiho Yoo, Sunggeon Ko, Hyeyon Kim, Heidi Sampson, Ji Hye Yun, Kwang Min Choe, Iksoo Chang, Cheryl H. Arrowsmith, Henry M. Krause, Hyun Soo Cho, Weontae Lee

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The interaction between the orphan nuclear receptor FTZ-F1 (Fushi tarazu factor 1) and the segmentation gene protein FTZ is critical for specifying alternate parasegments in the Drosophila embryo. Here, we have determined the structure of the FTZ-F1 ligand-binding domain (LBD)·FTZ peptide complex using x-ray crystallography. Strikingly, the ligand-binding pocket of the FTZ-F1LBDis completely occupied by helix 6 (H6) of the receptor, whereas the cofactor FTZ binds the co-activator cleft site of the FTZ-F1 LBD. Our findings suggest that H6 is essential for transcriptional activity of FTZ-F1; this is further supported by data from mutagenesis and activity assays. These data suggest that FTZ-F1 might belong to a novel class of ligand-independent nuclear receptors. Our findings are intriguing given that the highly homologous human steroidogenic factor-1 and liver receptor homolog-1 LBDs exhibit sizable ligand-binding pockets occupied by putative ligand molecules.

Original languageEnglish
Pages (from-to)31225-31231
Number of pages7
JournalJournal of Biological Chemistry
Volume286
Issue number36
DOIs
Publication statusPublished - 2011 Sep 9

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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