Pyrococcus furiosus PF2050 is an uncharacterized putative protein that contains two DUF2666 domains. Functional and structural studies of PF2050 have not previously been performed. In this study, we determined the crystal structure of PF2050. The structure of PF2050 showed that the two DUF2666 domains interact tightly, forming a globular structure. Each DUF2666 domain comprises an antiparallel β-sheet and an α-helical bundle. One side of the PF2050 structure has a positively charged basic cleft, which may have a DNA-binding function. Furthermore, we confirmed that PF2050 interacts with circular and linear dsDNA.
Bibliographical noteFunding Information:
We thank the staff at the Photon Factory (Japan) beamlines 5A and NW12A for assistance with the collection of the synchrotron data. This work was supported by the Basic Science Research Program ( 2009-0064219 and 2009-0074586 ), the Mid-career Researcher Program ( 2011-0029294 ) and the Bio & Medical Technology Development Program ( 2011-0030032 ) through the National Research Foundation of Korea (NRF), and was funded by the Ministry of Education, Science and Technology to S.L. and B.I.L.
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology
- Cell Biology