Crystallization and preliminary X-ray crystallographic study of HP1043, a Helicobacter pylori orphan response regulator

Hyang Mi Lee, Eunmi Hong, Byoung Young Jeon, Dong Uk Kim, Jung Sue Byun, Weontae Lee, Hyun Soo Cho

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The HP1043 protein of Helicobacter pylori is related to the two-component system that regulates prokaryotic transcription in response to environmental changes. The HP1043 gene encodes an orphan response regulator (RR). The N-terminal domain of HP1043 (HP1043N) was purified by affinity-column chromatography. The purified protein was crystallized by hanging drop vapor diffusion at 293 K. The crystal belongs to primitive orthorhombic space group P21212 with cell dimensions of a = 89.0, b = 41.3, and c = 31.7 Å. Assuming the presence of one molecule per asymmetric unit, the solvent content was estimated to be about 45%. A complete data set was collected at 1.8 Å resolution.

Original languageEnglish
Pages (from-to)989-991
Number of pages3
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1764
Issue number5
DOIs
Publication statusPublished - 2006 May

Bibliographical note

Funding Information:
Experiments at PLS were supported in part by MOST and POSTECH. We thank Sun-Sin Cha for assistance at beamline 6B of PLS. This work was supported in part by funds from the Korea Science and Engineering Foundation through the Protein Network Research Center and from a Korea Research Foundation Grant (KRF-2004-005-C00129), and from a Korea Research Foundation Grant funded by the Korean Government (R08-2004-000-10403-0). This work was supported by the NRL program of MOST NRDP (M1-0203-00-0020) (W.L.) from Korea Science and Engineering Foundation (KOSEF).

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biophysics
  • Biochemistry
  • Molecular Biology

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