The HP1043 protein of Helicobacter pylori is related to the two-component system that regulates prokaryotic transcription in response to environmental changes. The HP1043 gene encodes an orphan response regulator (RR). The N-terminal domain of HP1043 (HP1043N) was purified by affinity-column chromatography. The purified protein was crystallized by hanging drop vapor diffusion at 293 K. The crystal belongs to primitive orthorhombic space group P21212 with cell dimensions of a = 89.0, b = 41.3, and c = 31.7 Å. Assuming the presence of one molecule per asymmetric unit, the solvent content was estimated to be about 45%. A complete data set was collected at 1.8 Å resolution.
|Number of pages||3|
|Journal||Biochimica et Biophysica Acta - Proteins and Proteomics|
|Publication status||Published - 2006 May 1|
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Molecular Biology