Crystallization and preliminary X-ray crystallographic study of HP1043, a Helicobacter pylori orphan response regulator

Hyang Mi Lee, Eunmi Hong, Byoung Young Jeon, Dong Uk Kim, Jung Sue Byun, Weontae Lee, Hyun Soo Cho

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2 Citations (Scopus)

Abstract

The HP1043 protein of Helicobacter pylori is related to the two-component system that regulates prokaryotic transcription in response to environmental changes. The HP1043 gene encodes an orphan response regulator (RR). The N-terminal domain of HP1043 (HP1043N) was purified by affinity-column chromatography. The purified protein was crystallized by hanging drop vapor diffusion at 293 K. The crystal belongs to primitive orthorhombic space group P21212 with cell dimensions of a = 89.0, b = 41.3, and c = 31.7 Å. Assuming the presence of one molecule per asymmetric unit, the solvent content was estimated to be about 45%. A complete data set was collected at 1.8 Å resolution.

Original languageEnglish
Pages (from-to)989-991
Number of pages3
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1764
Issue number5
DOIs
Publication statusPublished - 2006 May 1

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biophysics
  • Biochemistry
  • Molecular Biology

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