CTX-M-14 and CTX-M-15 enzymes are the dominant type of extended-spectrum β-lactamase in clinical isolates of Escherichia coli from Korea

Wonkeun Song, Hyukmin Lee, Kyungwon Lee, Seok Hoon Jeong, II Kwon Bae, Jae Seok Kim, Hyo Sun Kwak

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

This study was performed to assess the prevalence and genotypes of plasmid-borne extended- spectrum β-lactamases (ESBLs) and AmpC β-lactamases in Escherichia coli in Korea. A total of 576 isolates of E. coli was collected from 12 Korean hospitals during May and July 2007. A phenotypic confirmatory test detected ESBLs in 82 (14.2 %) of the 576 E. coli isolates. The most common types of ESBLs identified were CTX-M-14 (n=32) and CTX-M-15 (n=27). The prevalence and diversity of the CTX- M mutants, including CTX-M-15, CTX-M-27 and CTX-M-57, with significant hydrolytic activity against ceftazidime were increased. PCR experiments detected genes encoding plasmid -borne AmpC β-lactamases in 15/56 cefoxitin -intermediate or cefoxitin- resistant isolates, and the most common type of AmpC β-lactamase identified was DHA-1 (n=10). These data suggest that the incidence of ESBLs in E. coli has increased as a result of the dissemination of CTX- M enzymes in Korea. In addition, CTX-M-22, CTX-M-27 and CTX-M-57 have appeared in Korea.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalJournal of Medical Microbiology
Volume58
Issue number2
DOIs
Publication statusPublished - 2009 Feb 1

Fingerprint

Korea
Escherichia coli
Cefoxitin
Enzymes
Plasmids
Ceftazidime
Genotype
Polymerase Chain Reaction
galantide
Incidence
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

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title = "CTX-M-14 and CTX-M-15 enzymes are the dominant type of extended-spectrum β-lactamase in clinical isolates of Escherichia coli from Korea",
abstract = "This study was performed to assess the prevalence and genotypes of plasmid-borne extended- spectrum β-lactamases (ESBLs) and AmpC β-lactamases in Escherichia coli in Korea. A total of 576 isolates of E. coli was collected from 12 Korean hospitals during May and July 2007. A phenotypic confirmatory test detected ESBLs in 82 (14.2 {\%}) of the 576 E. coli isolates. The most common types of ESBLs identified were CTX-M-14 (n=32) and CTX-M-15 (n=27). The prevalence and diversity of the CTX- M mutants, including CTX-M-15, CTX-M-27 and CTX-M-57, with significant hydrolytic activity against ceftazidime were increased. PCR experiments detected genes encoding plasmid -borne AmpC β-lactamases in 15/56 cefoxitin -intermediate or cefoxitin- resistant isolates, and the most common type of AmpC β-lactamase identified was DHA-1 (n=10). These data suggest that the incidence of ESBLs in E. coli has increased as a result of the dissemination of CTX- M enzymes in Korea. In addition, CTX-M-22, CTX-M-27 and CTX-M-57 have appeared in Korea.",
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CTX-M-14 and CTX-M-15 enzymes are the dominant type of extended-spectrum β-lactamase in clinical isolates of Escherichia coli from Korea. / Song, Wonkeun; Lee, Hyukmin; Lee, Kyungwon; Jeong, Seok Hoon; Bae, II Kwon; Kim, Jae Seok; Kwak, Hyo Sun.

In: Journal of Medical Microbiology, Vol. 58, No. 2, 01.02.2009, p. 261-266.

Research output: Contribution to journalArticle

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