TY - JOUR
T1 - CTX-M-14 and CTX-M-15 enzymes are the dominant type of extended-spectrum β-lactamase in clinical isolates of Escherichia coli from Korea
AU - Song, Wonkeun
AU - Lee, Hyukmin
AU - Lee, Kyungwon
AU - Jeong, Seok Hoon
AU - Bae, II Kwon
AU - Kim, Jae Seok
AU - Kwak, Hyo Sun
PY - 2009/2
Y1 - 2009/2
N2 - This study was performed to assess the prevalence and genotypes of plasmid-borne extended- spectrum β-lactamases (ESBLs) and AmpC β-lactamases in Escherichia coli in Korea. A total of 576 isolates of E. coli was collected from 12 Korean hospitals during May and July 2007. A phenotypic confirmatory test detected ESBLs in 82 (14.2 %) of the 576 E. coli isolates. The most common types of ESBLs identified were CTX-M-14 (n=32) and CTX-M-15 (n=27). The prevalence and diversity of the CTX- M mutants, including CTX-M-15, CTX-M-27 and CTX-M-57, with significant hydrolytic activity against ceftazidime were increased. PCR experiments detected genes encoding plasmid -borne AmpC β-lactamases in 15/56 cefoxitin -intermediate or cefoxitin- resistant isolates, and the most common type of AmpC β-lactamase identified was DHA-1 (n=10). These data suggest that the incidence of ESBLs in E. coli has increased as a result of the dissemination of CTX- M enzymes in Korea. In addition, CTX-M-22, CTX-M-27 and CTX-M-57 have appeared in Korea.
AB - This study was performed to assess the prevalence and genotypes of plasmid-borne extended- spectrum β-lactamases (ESBLs) and AmpC β-lactamases in Escherichia coli in Korea. A total of 576 isolates of E. coli was collected from 12 Korean hospitals during May and July 2007. A phenotypic confirmatory test detected ESBLs in 82 (14.2 %) of the 576 E. coli isolates. The most common types of ESBLs identified were CTX-M-14 (n=32) and CTX-M-15 (n=27). The prevalence and diversity of the CTX- M mutants, including CTX-M-15, CTX-M-27 and CTX-M-57, with significant hydrolytic activity against ceftazidime were increased. PCR experiments detected genes encoding plasmid -borne AmpC β-lactamases in 15/56 cefoxitin -intermediate or cefoxitin- resistant isolates, and the most common type of AmpC β-lactamase identified was DHA-1 (n=10). These data suggest that the incidence of ESBLs in E. coli has increased as a result of the dissemination of CTX- M enzymes in Korea. In addition, CTX-M-22, CTX-M-27 and CTX-M-57 have appeared in Korea.
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U2 - 10.1099/jmm.0.004507-0
DO - 10.1099/jmm.0.004507-0
M3 - Article
C2 - 19141747
AN - SCOPUS:59949098758
VL - 58
SP - 261
EP - 266
JO - Journal of Medical Microbiology
JF - Journal of Medical Microbiology
SN - 0022-2615
IS - 2
ER -