CTX-M-15 carried on Incn-type plasmids in Klebsiella pneumoniae

Zerrin Aktaş, Nevriye Gönüllü, Zeynep Çiǧdem Kayacan, Özdem Anǧ, Alessandra Carattoli, DongEun Yong, Timothy R. Walsh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective: The aim of this study was to characterize the CTX-M genes in clinical isolates of Klebsiella pneumoniae, particularly, the plasmid types that carry them. Material and Methods: Antimicrobial susceptibilities were determined by the agar dilution and E-test. Beta-lactamase production as analyzed by phenotypic tests [E-test MBL and extended spectrum β-lactamase (ESBL), isoelectric focusing, and bioassay] and molecular methods [polymerase chain reaction (PCR) detection of ESBL-encoding genes and IS elements, DNA sequencing and analysis of blaCTX-M and ISEcP1 PCR amplicons, typing by randomly amplified polymorphic DNA (RAPD) analysis and plasmid isolation, transformation, rep-typing and IncN plasmid confirmation]. Results: Thirty four (14.8%) out of 230 clinical isolates of K. pneumoniae were found as ESBL producers. The isolates produced one to five different β-lactamases, according to the isoelectric focusing results. The prevalence of the CTX-M-type ESBLs was found as 35% and sequencing proved all as CTX-M-15. RAPD analysis showed no clonal relation between the strains. Previous studies have shown that the blaCTX-M-15 gene was carried on FII plasmids. In 10 strains in this study, the CTX-M-15 gene was on 95 kb-larger plasmids typing to IncN. In two isolates the blaCTX-M-15 was carried on an approximately 60 kb plasmid and possessed an Inc/rep type of FII. Conclusion: This is the first report of IncN carrying blaCTX-M-15 and confirms the rapid emergence of CTX-M-15 enzymes among K. pneumoniae in Istanbul. Through this study, it was aimed to underline the risk of spread of IncN type plasmids, among gram-negative bacteria in Turkey, as shown previously in Greece.

Original languageEnglish
Pages (from-to)1355-1364
Number of pages10
JournalTurkiye Klinikleri Journal of Medical Sciences
Volume29
Issue number6
Publication statusPublished - 2009 Jan 1

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Klebsiella pneumoniae
Plasmids
Isoelectric Focusing
Genes
Polymerase Chain Reaction
DNA Transposable Elements
Greece
DNA
beta-Lactamases
Turkey
galantide
Gram-Negative Bacteria
DNA Sequence Analysis
Biological Assay
Agar
Enzymes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Aktaş, Z., Gönüllü, N., Kayacan, Z. Ç., Anǧ, Ö., Carattoli, A., Yong, D., & Walsh, T. R. (2009). CTX-M-15 carried on Incn-type plasmids in Klebsiella pneumoniae. Turkiye Klinikleri Journal of Medical Sciences, 29(6), 1355-1364.
Aktaş, Zerrin ; Gönüllü, Nevriye ; Kayacan, Zeynep Çiǧdem ; Anǧ, Özdem ; Carattoli, Alessandra ; Yong, DongEun ; Walsh, Timothy R. / CTX-M-15 carried on Incn-type plasmids in Klebsiella pneumoniae. In: Turkiye Klinikleri Journal of Medical Sciences. 2009 ; Vol. 29, No. 6. pp. 1355-1364.
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abstract = "Objective: The aim of this study was to characterize the CTX-M genes in clinical isolates of Klebsiella pneumoniae, particularly, the plasmid types that carry them. Material and Methods: Antimicrobial susceptibilities were determined by the agar dilution and E-test. Beta-lactamase production as analyzed by phenotypic tests [E-test MBL and extended spectrum β-lactamase (ESBL), isoelectric focusing, and bioassay] and molecular methods [polymerase chain reaction (PCR) detection of ESBL-encoding genes and IS elements, DNA sequencing and analysis of blaCTX-M and ISEcP1 PCR amplicons, typing by randomly amplified polymorphic DNA (RAPD) analysis and plasmid isolation, transformation, rep-typing and IncN plasmid confirmation]. Results: Thirty four (14.8{\%}) out of 230 clinical isolates of K. pneumoniae were found as ESBL producers. The isolates produced one to five different β-lactamases, according to the isoelectric focusing results. The prevalence of the CTX-M-type ESBLs was found as 35{\%} and sequencing proved all as CTX-M-15. RAPD analysis showed no clonal relation between the strains. Previous studies have shown that the blaCTX-M-15 gene was carried on FII plasmids. In 10 strains in this study, the CTX-M-15 gene was on 95 kb-larger plasmids typing to IncN. In two isolates the blaCTX-M-15 was carried on an approximately 60 kb plasmid and possessed an Inc/rep type of FII. Conclusion: This is the first report of IncN carrying blaCTX-M-15 and confirms the rapid emergence of CTX-M-15 enzymes among K. pneumoniae in Istanbul. Through this study, it was aimed to underline the risk of spread of IncN type plasmids, among gram-negative bacteria in Turkey, as shown previously in Greece.",
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Aktaş, Z, Gönüllü, N, Kayacan, ZÇ, Anǧ, Ö, Carattoli, A, Yong, D & Walsh, TR 2009, 'CTX-M-15 carried on Incn-type plasmids in Klebsiella pneumoniae', Turkiye Klinikleri Journal of Medical Sciences, vol. 29, no. 6, pp. 1355-1364.

CTX-M-15 carried on Incn-type plasmids in Klebsiella pneumoniae. / Aktaş, Zerrin; Gönüllü, Nevriye; Kayacan, Zeynep Çiǧdem; Anǧ, Özdem; Carattoli, Alessandra; Yong, DongEun; Walsh, Timothy R.

In: Turkiye Klinikleri Journal of Medical Sciences, Vol. 29, No. 6, 01.01.2009, p. 1355-1364.

Research output: Contribution to journalArticle

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AU - Aktaş, Zerrin

AU - Gönüllü, Nevriye

AU - Kayacan, Zeynep Çiǧdem

AU - Anǧ, Özdem

AU - Carattoli, Alessandra

AU - Yong, DongEun

AU - Walsh, Timothy R.

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N2 - Objective: The aim of this study was to characterize the CTX-M genes in clinical isolates of Klebsiella pneumoniae, particularly, the plasmid types that carry them. Material and Methods: Antimicrobial susceptibilities were determined by the agar dilution and E-test. Beta-lactamase production as analyzed by phenotypic tests [E-test MBL and extended spectrum β-lactamase (ESBL), isoelectric focusing, and bioassay] and molecular methods [polymerase chain reaction (PCR) detection of ESBL-encoding genes and IS elements, DNA sequencing and analysis of blaCTX-M and ISEcP1 PCR amplicons, typing by randomly amplified polymorphic DNA (RAPD) analysis and plasmid isolation, transformation, rep-typing and IncN plasmid confirmation]. Results: Thirty four (14.8%) out of 230 clinical isolates of K. pneumoniae were found as ESBL producers. The isolates produced one to five different β-lactamases, according to the isoelectric focusing results. The prevalence of the CTX-M-type ESBLs was found as 35% and sequencing proved all as CTX-M-15. RAPD analysis showed no clonal relation between the strains. Previous studies have shown that the blaCTX-M-15 gene was carried on FII plasmids. In 10 strains in this study, the CTX-M-15 gene was on 95 kb-larger plasmids typing to IncN. In two isolates the blaCTX-M-15 was carried on an approximately 60 kb plasmid and possessed an Inc/rep type of FII. Conclusion: This is the first report of IncN carrying blaCTX-M-15 and confirms the rapid emergence of CTX-M-15 enzymes among K. pneumoniae in Istanbul. Through this study, it was aimed to underline the risk of spread of IncN type plasmids, among gram-negative bacteria in Turkey, as shown previously in Greece.

AB - Objective: The aim of this study was to characterize the CTX-M genes in clinical isolates of Klebsiella pneumoniae, particularly, the plasmid types that carry them. Material and Methods: Antimicrobial susceptibilities were determined by the agar dilution and E-test. Beta-lactamase production as analyzed by phenotypic tests [E-test MBL and extended spectrum β-lactamase (ESBL), isoelectric focusing, and bioassay] and molecular methods [polymerase chain reaction (PCR) detection of ESBL-encoding genes and IS elements, DNA sequencing and analysis of blaCTX-M and ISEcP1 PCR amplicons, typing by randomly amplified polymorphic DNA (RAPD) analysis and plasmid isolation, transformation, rep-typing and IncN plasmid confirmation]. Results: Thirty four (14.8%) out of 230 clinical isolates of K. pneumoniae were found as ESBL producers. The isolates produced one to five different β-lactamases, according to the isoelectric focusing results. The prevalence of the CTX-M-type ESBLs was found as 35% and sequencing proved all as CTX-M-15. RAPD analysis showed no clonal relation between the strains. Previous studies have shown that the blaCTX-M-15 gene was carried on FII plasmids. In 10 strains in this study, the CTX-M-15 gene was on 95 kb-larger plasmids typing to IncN. In two isolates the blaCTX-M-15 was carried on an approximately 60 kb plasmid and possessed an Inc/rep type of FII. Conclusion: This is the first report of IncN carrying blaCTX-M-15 and confirms the rapid emergence of CTX-M-15 enzymes among K. pneumoniae in Istanbul. Through this study, it was aimed to underline the risk of spread of IncN type plasmids, among gram-negative bacteria in Turkey, as shown previously in Greece.

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Aktaş Z, Gönüllü N, Kayacan ZÇ, Anǧ Ö, Carattoli A, Yong D et al. CTX-M-15 carried on Incn-type plasmids in Klebsiella pneumoniae. Turkiye Klinikleri Journal of Medical Sciences. 2009 Jan 1;29(6):1355-1364.