Cultivation of microalgal biomass using swine manure for biohydrogen production: Impact of dilution ratio and pretreatment

Gopalakrishnan Kumar, Dinh Duc Nguyen, Periyasamy Sivagurunathan, Takuro Kobayashi, Kaiqin Xu, Soon Woong Chang

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38 Citations (Scopus)


This study assessed the impact of swine manure (SM) dilution ratio on the microalgal biomass cultivation and further tested for biohydrogen production efficiency from the mixed microalgal biomass. At first, various solid/liquid (S/L) ratio of the SM ranged from 2.5 to 10 g/L was prepared as a nutrient medium for the algal biomass cultivation without addition of the external nutrient sources over a period of 18 d. The peak biomass concentration of 2.57 ± 0.03 g/L was obtained under the initial S/L loading rates of 5 g/L. Further, the cultivated biomass was subjected to two-step (ultrasonication + enzymatic) pretreatment and evaluated for biohydrogen production potential. Results showed that the variable amount of hydrogen production was observed with different S/L ratio of the SM. The peak hydrogen yield of 116 ± 6 mL/g TSadded was observed at the 5 g/L grown SM mixed algal biomass.

Original languageEnglish
Pages (from-to)16-22
Number of pages7
JournalBioresource technology
Publication statusPublished - 2018 Jul

Bibliographical note

Funding Information:
The author Dr. Periyasamy Sivagurunathan greatly acknowledged the financial assistance from Japan Society for Promotion of Science: JSPS ID: P15370 for this study. The swine manure provided by Prof. Zhenya Zhang and Prof. Zhongfang Lei, University of Tsukuba, Ibaraki, Tsukuba, Japan is gratefully acknowledged. This work was supported in part by grants from the Korea Research Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (Grant No: 2016H1D3A1908953 ), the New & Renewable Energy Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) granted financial resource from the Ministry of Trade, Industry & Energy, Republic of Korea (Project No. 20173010092470), and the Institute for Information & Communications Technology Promotion (IITP) grant funded by the Korea government (MSIP) (Project No. 2017-0-00372).

Publisher Copyright:
© 2018 Elsevier Ltd

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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