Abstract
The aim of this study was to fabricate porous chitosan microbeads using an electrospraying method into liquid nitrogen, then thawing and refreezing. The microbeads were then used to evaluate their potential for tissue engineering of human mesenchymal stem cells (hMSCs). Scanning electron microscopy (SEM) and a mercury porosimeter were used to show the morphology of the scaffolds formed and to determine their pore size and porosity. As the chitosan concentration increased (0.5, 1, 1.5, and 2 wt %), the diameter of the porous microbead increased from 350 to 890 lm, and the average pore size and the porosity decreased from 65 to 21 lm and 95 to 38%, respectively. The hMSCs were cultured onto the porous microbeads in a spinner flask. SEM images and methyl tetrazolium salt assays at 3, 7, 14, and 21 days of culture revealed that hMSCs had successfully attached and proliferated inside the porous microbeads. This study demonstrated that electrosprayed porous chitosan microbeads can be used as three-dimensional scaffolds for tissue engineering.
| Original language | English |
|---|---|
| Pages (from-to) | 869-876 |
| Number of pages | 8 |
| Journal | Journal of Biomedical Materials Research - Part A |
| Volume | 92 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2010 Mar 1 |
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All Science Journal Classification (ASJC) codes
- Ceramics and Composites
- Biomaterials
- Biomedical Engineering
- Metals and Alloys
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Culture of human mesenchymal stem cells using electrosprayed porous chitosan microbeads. / Maeng, Yu Jeong; Choi, Sung Wook; Kim, Hyun Ok; Kim, Jung Hyun.
In: Journal of Biomedical Materials Research - Part A, Vol. 92, No. 3, 01.03.2010, p. 869-876.Research output: Contribution to journal › Article
TY - JOUR
T1 - Culture of human mesenchymal stem cells using electrosprayed porous chitosan microbeads
AU - Maeng, Yu Jeong
AU - Choi, Sung Wook
AU - Kim, Hyun Ok
AU - Kim, Jung Hyun
PY - 2010/3/1
Y1 - 2010/3/1
N2 - The aim of this study was to fabricate porous chitosan microbeads using an electrospraying method into liquid nitrogen, then thawing and refreezing. The microbeads were then used to evaluate their potential for tissue engineering of human mesenchymal stem cells (hMSCs). Scanning electron microscopy (SEM) and a mercury porosimeter were used to show the morphology of the scaffolds formed and to determine their pore size and porosity. As the chitosan concentration increased (0.5, 1, 1.5, and 2 wt %), the diameter of the porous microbead increased from 350 to 890 lm, and the average pore size and the porosity decreased from 65 to 21 lm and 95 to 38%, respectively. The hMSCs were cultured onto the porous microbeads in a spinner flask. SEM images and methyl tetrazolium salt assays at 3, 7, 14, and 21 days of culture revealed that hMSCs had successfully attached and proliferated inside the porous microbeads. This study demonstrated that electrosprayed porous chitosan microbeads can be used as three-dimensional scaffolds for tissue engineering.
AB - The aim of this study was to fabricate porous chitosan microbeads using an electrospraying method into liquid nitrogen, then thawing and refreezing. The microbeads were then used to evaluate their potential for tissue engineering of human mesenchymal stem cells (hMSCs). Scanning electron microscopy (SEM) and a mercury porosimeter were used to show the morphology of the scaffolds formed and to determine their pore size and porosity. As the chitosan concentration increased (0.5, 1, 1.5, and 2 wt %), the diameter of the porous microbead increased from 350 to 890 lm, and the average pore size and the porosity decreased from 65 to 21 lm and 95 to 38%, respectively. The hMSCs were cultured onto the porous microbeads in a spinner flask. SEM images and methyl tetrazolium salt assays at 3, 7, 14, and 21 days of culture revealed that hMSCs had successfully attached and proliferated inside the porous microbeads. This study demonstrated that electrosprayed porous chitosan microbeads can be used as three-dimensional scaffolds for tissue engineering.
UR - http://www.scopus.com/inward/record.url?scp=75749152984&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=75749152984&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.32417
DO - 10.1002/jbm.a.32417
M3 - Article
C2 - 19280627
AN - SCOPUS:75749152984
VL - 92
SP - 869
EP - 876
JO - Journal of Biomedical Materials Research
JF - Journal of Biomedical Materials Research
SN - 1549-3296
IS - 3
ER -