Culture on a 3,4-dihydroxy-L-phenylalanine-coated surface promotes the osteogenic differentiation of human mesenchymal stem cells

Wan Geun La, Jung Youn Shin, Suk Ho Bhang, Min Jin, Hee Hun Yoon, Seong Seo Noh, Gun Il Im, Chang Sung Kim, Byung Soo Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The culture surface can affect the in vitro differentiation of stem cells. In this study, we investigated whether modifying the culture surface with 3,4-dihydroxy-L-phenylalanine (DOPA), an element of mussel adhesion protein, could enhance the in vitro osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMMSCs). hBMMSCs cultured on DOPA-coated plates exhibited better cell adhesion and spreading compared with noncoated conventional tissue culture plates. The DOPA coated did not affect the apoptosis or viability of the cultured hBMMSCs. Also, hBMMSCs cultured on DOPA-coated plates exhibited a higher degree of osteogenic differentiation than did hBMMSCs cultured on noncoated plates, as evaluated with alkaline phosphate (ALP) activity, calcium content, and the mRNA expression of runt-related transcription factor 2, ALP, and osteocalcin. Further, hBMMSCs cultured on DOPA-coated plates demonstrated a higher capability of ectopic bone formation in vivo following implantation in the subcutaneous space of athymic mice compared with hBMMSCs cultured on noncoated plates, as evaluated with microcomputer topography analysis and histomorphometry. These results indicate that modifying the culture surface with DOPA can enhance the in vitro osteogenic differentiation of hBMMSCs.

Original languageEnglish
Pages (from-to)1255-1263
Number of pages9
JournalTissue Engineering - Part A
Volume19
Issue number9-10
DOIs
Publication statusPublished - 2013 May 1

Fingerprint

Stem cells
Phenylalanine
Mesenchymal Stromal Cells
Bone
Bone Marrow
Phosphates
Bivalvia
Osteocalcin
Tissue culture
Microcomputers
Transcription factors
Cell adhesion
Osteogenesis
Nude Mice
Cell Adhesion
Cell death
Cell culture
Topography
Transcription Factors
Stem Cells

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

La, Wan Geun ; Shin, Jung Youn ; Bhang, Suk Ho ; Jin, Min ; Yoon, Hee Hun ; Noh, Seong Seo ; Im, Gun Il ; Kim, Chang Sung ; Kim, Byung Soo. / Culture on a 3,4-dihydroxy-L-phenylalanine-coated surface promotes the osteogenic differentiation of human mesenchymal stem cells. In: Tissue Engineering - Part A. 2013 ; Vol. 19, No. 9-10. pp. 1255-1263.
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Culture on a 3,4-dihydroxy-L-phenylalanine-coated surface promotes the osteogenic differentiation of human mesenchymal stem cells. / La, Wan Geun; Shin, Jung Youn; Bhang, Suk Ho; Jin, Min; Yoon, Hee Hun; Noh, Seong Seo; Im, Gun Il; Kim, Chang Sung; Kim, Byung Soo.

In: Tissue Engineering - Part A, Vol. 19, No. 9-10, 01.05.2013, p. 1255-1263.

Research output: Contribution to journalArticle

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AU - Yoon, Hee Hun

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AU - Im, Gun Il

AU - Kim, Chang Sung

AU - Kim, Byung Soo

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