Abstract
Background Cell therapy for cardiovascular disease has been limited by low engraftment of administered cellsand modest therapeutic effects. Bone marrow (BM) -derived CD31+ cells are a promising cell source owing to theirhigh angiovasculogenic and paracrine activities.
Results The CD31+ cells cultured in endothelial cell medium (EC-CD31+ cells) showed the highest adhesion andangiogenic activities and lowest inflammatory properties in vitro compared with uncultured or other cultured CD31+ cells.When implanted into mouse MI or HLI models, EC-CD31+ cells improved cardiac function and repaired limb ischemiato a greater extent than uncultured CD31+ cells. Histologically, injected EC-CD31+ cells exhibited higher retention,neovascularization, and cardiomyocyte proliferation. Importantly, cell retention and endothelial transdifferentiationwas sustained up to 1 year.
Objectives This study sought to identify culture conditions that could augment the cell adhesion, angiogenic, andanti-inflammatory activities of BM-derived CD31+ cells, and to determine whether these cultured CD31+ cells areeffective for cardiac and vascular repair.
Methods CD31+ cells were isolated from human BM by magnetic-activated cell sorting and cultured for 10 daysunder hematopoietic stem cell, mesenchymal stem cell, or endothelial cell culture conditions. These cells werecharacterized by adhesion, angiogenesis, and inflammatory assays. The best of the cultured cells were implantedinto myocardial infarction (MI) and hindlimb ischemia (HLI) models to determine therapeutic effects and underlyingmechanisms.
Conclusions Short-term cultured EC-CD31+ cells have higher cell engraftment, vessel-formation, cardiomyocyteproliferation, and anti-inflammatory potential, are highly effective for both cardiac and peripheral vascularrepair, and enhance survival of mice with heart failure. These cultured CD31+ cells may be a promising source fortreating ischemic cardiovascular diseases. (J Am Coll Cardiol 2014;64:168194)
| Original language | English |
|---|---|
| Pages (from-to) | 1681-1694 |
| Number of pages | 14 |
| Journal | Journal of the American College of Cardiology |
| Volume | 64 |
| Issue number | 16 |
| DOIs | |
| Publication status | Published - 2014 Oct 21 |
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All Science Journal Classification (ASJC) codes
- Cardiology and Cardiovascular Medicine
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Cultured human bone marrow-derived CD31+ cells are effective for cardiac and vascular repair through enhanced angiogenic, adhesion, and anti-inflammatory effects. / Kim, Sung Whan; Houge, Mackenzie; Brown, Milton; Davis, Michael E.; Yoon, Young Sup.
In: Journal of the American College of Cardiology, Vol. 64, No. 16, 21.10.2014, p. 1681-1694.Research output: Contribution to journal › Article
TY - JOUR
T1 - Cultured human bone marrow-derived CD31+ cells are effective for cardiac and vascular repair through enhanced angiogenic, adhesion, and anti-inflammatory effects
AU - Kim, Sung Whan
AU - Houge, Mackenzie
AU - Brown, Milton
AU - Davis, Michael E.
AU - Yoon, Young Sup
PY - 2014/10/21
Y1 - 2014/10/21
N2 - Background Cell therapy for cardiovascular disease has been limited by low engraftment of administered cellsand modest therapeutic effects. Bone marrow (BM) -derived CD31+ cells are a promising cell source owing to theirhigh angiovasculogenic and paracrine activities.Results The CD31+ cells cultured in endothelial cell medium (EC-CD31+ cells) showed the highest adhesion andangiogenic activities and lowest inflammatory properties in vitro compared with uncultured or other cultured CD31+ cells.When implanted into mouse MI or HLI models, EC-CD31+ cells improved cardiac function and repaired limb ischemiato a greater extent than uncultured CD31+ cells. Histologically, injected EC-CD31+ cells exhibited higher retention,neovascularization, and cardiomyocyte proliferation. Importantly, cell retention and endothelial transdifferentiationwas sustained up to 1 year.Objectives This study sought to identify culture conditions that could augment the cell adhesion, angiogenic, andanti-inflammatory activities of BM-derived CD31+ cells, and to determine whether these cultured CD31+ cells areeffective for cardiac and vascular repair.Methods CD31+ cells were isolated from human BM by magnetic-activated cell sorting and cultured for 10 daysunder hematopoietic stem cell, mesenchymal stem cell, or endothelial cell culture conditions. These cells werecharacterized by adhesion, angiogenesis, and inflammatory assays. The best of the cultured cells were implantedinto myocardial infarction (MI) and hindlimb ischemia (HLI) models to determine therapeutic effects and underlyingmechanisms.Conclusions Short-term cultured EC-CD31+ cells have higher cell engraftment, vessel-formation, cardiomyocyteproliferation, and anti-inflammatory potential, are highly effective for both cardiac and peripheral vascularrepair, and enhance survival of mice with heart failure. These cultured CD31+ cells may be a promising source fortreating ischemic cardiovascular diseases. (J Am Coll Cardiol 2014;64:168194)
AB - Background Cell therapy for cardiovascular disease has been limited by low engraftment of administered cellsand modest therapeutic effects. Bone marrow (BM) -derived CD31+ cells are a promising cell source owing to theirhigh angiovasculogenic and paracrine activities.Results The CD31+ cells cultured in endothelial cell medium (EC-CD31+ cells) showed the highest adhesion andangiogenic activities and lowest inflammatory properties in vitro compared with uncultured or other cultured CD31+ cells.When implanted into mouse MI or HLI models, EC-CD31+ cells improved cardiac function and repaired limb ischemiato a greater extent than uncultured CD31+ cells. Histologically, injected EC-CD31+ cells exhibited higher retention,neovascularization, and cardiomyocyte proliferation. Importantly, cell retention and endothelial transdifferentiationwas sustained up to 1 year.Objectives This study sought to identify culture conditions that could augment the cell adhesion, angiogenic, andanti-inflammatory activities of BM-derived CD31+ cells, and to determine whether these cultured CD31+ cells areeffective for cardiac and vascular repair.Methods CD31+ cells were isolated from human BM by magnetic-activated cell sorting and cultured for 10 daysunder hematopoietic stem cell, mesenchymal stem cell, or endothelial cell culture conditions. These cells werecharacterized by adhesion, angiogenesis, and inflammatory assays. The best of the cultured cells were implantedinto myocardial infarction (MI) and hindlimb ischemia (HLI) models to determine therapeutic effects and underlyingmechanisms.Conclusions Short-term cultured EC-CD31+ cells have higher cell engraftment, vessel-formation, cardiomyocyteproliferation, and anti-inflammatory potential, are highly effective for both cardiac and peripheral vascularrepair, and enhance survival of mice with heart failure. These cultured CD31+ cells may be a promising source fortreating ischemic cardiovascular diseases. (J Am Coll Cardiol 2014;64:168194)
UR - http://www.scopus.com/inward/record.url?scp=84907969847&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907969847&partnerID=8YFLogxK
U2 - 10.1016/j.jacc.2014.06.1204
DO - 10.1016/j.jacc.2014.06.1204
M3 - Article
C2 - 25323256
AN - SCOPUS:84907969847
VL - 64
SP - 1681
EP - 1694
JO - Journal of the American College of Cardiology
JF - Journal of the American College of Cardiology
SN - 0735-1097
IS - 16
ER -