Cyclomaltodextrinase, neopullulanase, and maltogenic amylase are nearly indistinguishable from each other

Hee Seob Lee, Min Sung Kim, Hyun Soo Cho, Jung In Kim, Tae Jip Kim, Ji Hye Choi, Cheonseok Park, Heung Soo Lee, Byung Ha Oh, Kwan Hwa Park

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Abstract

Over 20 enzymes denoted as cyclomaltodextrinase, maltogenic amylase, or neopullulanase that share 40-86% sequence identity with each other are found in public data bases. These enzymes are distinguished from typical α-amylases by containing a novel N-terminal domain and exhibiting preferential substrate specificities for cyclomaltodextrins (CDs) over starch. In this research field, a great deal of confusion exists regarding the features distinguishing the three groups of enzymes from one another. Although a different enzyme code has been assigned to each of the three different enzyme names, even a single differentiating enzymatic property has not been documented in the literature. On the other hand, an outstanding question related to this issue concerns the structural basis for the preference of these enzymes for CDs. To clarify the confusion and to address this question, we have determined the structures of two enzymes, one from alkalophilic Bacillus sp. I-5 and named cyclomaltodextrinase and the other from a Thermus species and named maltogenic amylase. The structure of the Bacillus enzyme reveals a dodecameric assembly composed of six copies of the dimer, which is the structural and functional unit of the Thermus enzyme and an enzyme named neopullulanase. The structure of the Thermus enzyme in complex with β-CD led to the conclusion that Trp 47, a well conserved N-terminal domain residue, contributes greatly to the preference for β-CD. The common dimer formation through the novel N-terminal domain, which contributes to the preference for CDs by lining the active-site cavity, convincingly indicates that the three groups of enzymes are not different enough to preserve the different names and enzyme codes.

Original languageEnglish
Pages (from-to)21891-21897
Number of pages7
JournalJournal of Biological Chemistry
Volume277
Issue number24
DOIs
Publication statusPublished - 2002 Jun 14

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Lee, H. S., Kim, M. S., Cho, H. S., Kim, J. I., Kim, T. J., Choi, J. H., Park, C., Lee, H. S., Oh, B. H., & Park, K. H. (2002). Cyclomaltodextrinase, neopullulanase, and maltogenic amylase are nearly indistinguishable from each other. Journal of Biological Chemistry, 277(24), 21891-21897. https://doi.org/10.1074/jbc.M201623200