Cytokine Expression of Stem Cells Originating from the Apical Complex and Coronal Pulp of Immature Teeth

Ki Hoon Joo, Je Seon Song, Seunghye Kim, Hyo Seol Lee, Mijeong Jeon, Seong Oh Kim, Jae Ho Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Introduction The aim of this study was to measure and compare the expression levels of cytokines from developing apical complex cells (DACCs) and dental pulp stem cells (DPSCs) of the immature tooth. Methods DPSC-conditioned medium (CM) and DACCs-CM were obtained from human young teeth, and 174 cytokines secreted from each CM were identified and compared. A cytokine membrane array and enzyme-linked immunosorbent assay were used to measure and compare the expression levels of the cytokines. Immunocytochemistry targeting insulin-like growth factor-1 and neurotrophin-3 was additionally performed. Results There were statistically significant differences in the expression levels of 25 cytokines: 22 and 3 were expressed more strongly in DPSCs-CM and DACCs-CM, respectively. Odontoblast differentiation-related cytokines were more strongly expressed in DPSCs-CM, while cell-proliferation–related cytokines were more strongly expressed in DACCs-CM. Proinflammatory and anti-inflammatory cytokines were predominantly expressed in DPSCs-CM and DACCs-CM, respectively. Conclusions DPSCs may exert a stronger paracrine effect than DACCs on regeneration of the dentin–pulp complex, in terms of odontoblast differentiation.

Original languageEnglish
Pages (from-to)87-92.e1
JournalJournal of Endodontics
Volume44
Issue number1
DOIs
Publication statusPublished - 2018 Jan

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Conditioned Culture Medium
Dental Pulp
Tooth
Stem Cells
Cytokines
Odontoblasts
Neurotrophin 3
Somatomedins
Regeneration
Anti-Inflammatory Agents
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Membranes

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Joo, Ki Hoon ; Song, Je Seon ; Kim, Seunghye ; Lee, Hyo Seol ; Jeon, Mijeong ; Kim, Seong Oh ; Lee, Jae Ho. / Cytokine Expression of Stem Cells Originating from the Apical Complex and Coronal Pulp of Immature Teeth. In: Journal of Endodontics. 2018 ; Vol. 44, No. 1. pp. 87-92.e1.
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Cytokine Expression of Stem Cells Originating from the Apical Complex and Coronal Pulp of Immature Teeth. / Joo, Ki Hoon; Song, Je Seon; Kim, Seunghye; Lee, Hyo Seol; Jeon, Mijeong; Kim, Seong Oh; Lee, Jae Ho.

In: Journal of Endodontics, Vol. 44, No. 1, 01.2018, p. 87-92.e1.

Research output: Contribution to journalArticle

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AU - Song, Je Seon

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AU - Jeon, Mijeong

AU - Kim, Seong Oh

AU - Lee, Jae Ho

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N2 - Introduction The aim of this study was to measure and compare the expression levels of cytokines from developing apical complex cells (DACCs) and dental pulp stem cells (DPSCs) of the immature tooth. Methods DPSC-conditioned medium (CM) and DACCs-CM were obtained from human young teeth, and 174 cytokines secreted from each CM were identified and compared. A cytokine membrane array and enzyme-linked immunosorbent assay were used to measure and compare the expression levels of the cytokines. Immunocytochemistry targeting insulin-like growth factor-1 and neurotrophin-3 was additionally performed. Results There were statistically significant differences in the expression levels of 25 cytokines: 22 and 3 were expressed more strongly in DPSCs-CM and DACCs-CM, respectively. Odontoblast differentiation-related cytokines were more strongly expressed in DPSCs-CM, while cell-proliferation–related cytokines were more strongly expressed in DACCs-CM. Proinflammatory and anti-inflammatory cytokines were predominantly expressed in DPSCs-CM and DACCs-CM, respectively. Conclusions DPSCs may exert a stronger paracrine effect than DACCs on regeneration of the dentin–pulp complex, in terms of odontoblast differentiation.

AB - Introduction The aim of this study was to measure and compare the expression levels of cytokines from developing apical complex cells (DACCs) and dental pulp stem cells (DPSCs) of the immature tooth. Methods DPSC-conditioned medium (CM) and DACCs-CM were obtained from human young teeth, and 174 cytokines secreted from each CM were identified and compared. A cytokine membrane array and enzyme-linked immunosorbent assay were used to measure and compare the expression levels of the cytokines. Immunocytochemistry targeting insulin-like growth factor-1 and neurotrophin-3 was additionally performed. Results There were statistically significant differences in the expression levels of 25 cytokines: 22 and 3 were expressed more strongly in DPSCs-CM and DACCs-CM, respectively. Odontoblast differentiation-related cytokines were more strongly expressed in DPSCs-CM, while cell-proliferation–related cytokines were more strongly expressed in DACCs-CM. Proinflammatory and anti-inflammatory cytokines were predominantly expressed in DPSCs-CM and DACCs-CM, respectively. Conclusions DPSCs may exert a stronger paracrine effect than DACCs on regeneration of the dentin–pulp complex, in terms of odontoblast differentiation.

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