In response to genotoxic stress, the tumor suppressor protein p73 induces apoptosis and cell cycle arrest. Despite extensive studies on p73-mediated apoptosis, little is known about the cytoplasmic apoptotic function of p73. Here, using H1299 lung cancer cells and diverse biochemical approaches, including colony formation,DNAfragmentation,GSTpulldown,andapoptosis assays along with NMR spectroscopy, we show that p73 induces transcription-independent apoptosis via its transactivation domain (TAD) through a mitochondrial pathway and that this apoptosis is mediated by the interaction between p73- TAD and the anti-apoptotic protein B-cell lymphoma-extra large (Bcl-XL or BCL2L1). This binding disrupted an interaction between Bcl-XL and the pro-apoptotic protein BH3-interacting domain death agonist (Bid). In particular, we found that a 16-mer p73-TAD peptide motif (p73-TAD16) mediates transcription-independent apoptosis, accompanied by cytochrome c release from the mitochondria, by interacting with Bcl-XL. Interestingly, the structure of the Bcl-XL-p73-TAD16 peptide complex revealed a novel mechanism of Bcl-XL recognition by p73-TAD.Weobserved that theα-helical p73-TAD16 peptide binds to a noncanonical site in Bcl-XL, comprising the BH1, BH2, and BH3 domains in an orientation opposite to those of pro-apoptoticBH3peptides. Taken together, our results indicate that the cytoplasmic apoptotic function of p73 is mediated through a noncanonical mode of Bcl-XL recognition. This finding sheds light on a critical transcription-independent, p73-mediated mechanism for apoptosis induction, which has potential implications for anticancer therapy.
Bibliographical noteFunding Information:
This work was supported by Grants NRF-2017R1E1A1A01074403, NRF-2017M3A9C4092979, and NRF-2017R1A2B4006378 from the National Research Foundation of Korea funded by the Korean Government (MSIT) and by the KRIBB Research Initiative Program. This work was also sup-ported by Grant 10038744 from the Technology Innovation Program of Korea Evaluation Institute of Industrial Technology funded by Ministry of Trade, Industry & Energy, Republic of Korea. The authors declare that they have no conflicts of interest with the contents of this article.
© 2018 Yoon et al.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology