Cytotoxicity of four denture adhesives on human gingival fibroblast cells

Yoon Lee, Jin Soo Ahn, Young A.H. Yi, Shin Hye Chung, Yeon Jee Yoo, Sung Won Ju, J. I.Yun Hwang, Deog Gyu Seo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective. The purpose of this study was to compare the cytotoxicity of four denture adhesives on human gingival fibroblast cells. Materials and methods. Immortalized human gingival fibroblasts were cultured with one of four different denture adhesives, Polident, Protefix, Staydent or Denfix-A, which was placed in insert dishes (10% w/v concentration) for 48 h. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and flow cytometric apoptosis assay were used to evaluate cell viability and apoptosis rates. The fibroblasts were also examined under a scanning electron microscope. Results. The MTT assay showed that all denture adhesives resulted in a signi ficantly lower cell viability compared to the control cells propagated in normal culture medium (p < 0.05), with Staydent demonstrating the lowest cell viability. According to the flow cytometric apoptosis assay, Staydent and Protefix showed significantly higher apoptosis rates than the control group (p < 0.05), whereas Polident and Denfix-A did not demonstrate any significant differences (p > 0.05). Staydent showed the highest apoptosis rate. Scanning electron microscopy showed that the cells of the Staydent group underwent cytoplasmic membrane shrinkage, with cell free areas containing residual fragments of the membrane of dead cells. Conclusions. The four denture adhesives evaluated in this study imparted cytotoxic effects on human gingival fibroblast cells. Staydent showed the highest toxicity.

Original languageEnglish
Pages (from-to)87-92
Number of pages6
JournalActa odontologica Scandinavica
Volume73
Issue number2
DOIs
Publication statusPublished - 2014 Apr 1

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Dentures
Adhesives
Fibroblasts
Apoptosis
Cell Survival
Cell Membrane
Electron Scanning Microscopy
Culture Media
Electrons

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Lee, Y., Ahn, J. S., Yi, Y. A. H., Chung, S. H., Yoo, Y. J., Ju, S. W., ... Seo, D. G. (2014). Cytotoxicity of four denture adhesives on human gingival fibroblast cells. Acta odontologica Scandinavica, 73(2), 87-92. https://doi.org/10.3109/00016357.2014.954266
Lee, Yoon ; Ahn, Jin Soo ; Yi, Young A.H. ; Chung, Shin Hye ; Yoo, Yeon Jee ; Ju, Sung Won ; Hwang, J. I.Yun ; Seo, Deog Gyu. / Cytotoxicity of four denture adhesives on human gingival fibroblast cells. In: Acta odontologica Scandinavica. 2014 ; Vol. 73, No. 2. pp. 87-92.
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abstract = "Objective. The purpose of this study was to compare the cytotoxicity of four denture adhesives on human gingival fibroblast cells. Materials and methods. Immortalized human gingival fibroblasts were cultured with one of four different denture adhesives, Polident, Protefix, Staydent or Denfix-A, which was placed in insert dishes (10{\%} w/v concentration) for 48 h. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and flow cytometric apoptosis assay were used to evaluate cell viability and apoptosis rates. The fibroblasts were also examined under a scanning electron microscope. Results. The MTT assay showed that all denture adhesives resulted in a signi ficantly lower cell viability compared to the control cells propagated in normal culture medium (p < 0.05), with Staydent demonstrating the lowest cell viability. According to the flow cytometric apoptosis assay, Staydent and Protefix showed significantly higher apoptosis rates than the control group (p < 0.05), whereas Polident and Denfix-A did not demonstrate any significant differences (p > 0.05). Staydent showed the highest apoptosis rate. Scanning electron microscopy showed that the cells of the Staydent group underwent cytoplasmic membrane shrinkage, with cell free areas containing residual fragments of the membrane of dead cells. Conclusions. The four denture adhesives evaluated in this study imparted cytotoxic effects on human gingival fibroblast cells. Staydent showed the highest toxicity.",
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Lee, Y, Ahn, JS, Yi, YAH, Chung, SH, Yoo, YJ, Ju, SW, Hwang, JIY & Seo, DG 2014, 'Cytotoxicity of four denture adhesives on human gingival fibroblast cells', Acta odontologica Scandinavica, vol. 73, no. 2, pp. 87-92. https://doi.org/10.3109/00016357.2014.954266

Cytotoxicity of four denture adhesives on human gingival fibroblast cells. / Lee, Yoon; Ahn, Jin Soo; Yi, Young A.H.; Chung, Shin Hye; Yoo, Yeon Jee; Ju, Sung Won; Hwang, J. I.Yun; Seo, Deog Gyu.

In: Acta odontologica Scandinavica, Vol. 73, No. 2, 01.04.2014, p. 87-92.

Research output: Contribution to journalArticle

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T1 - Cytotoxicity of four denture adhesives on human gingival fibroblast cells

AU - Lee, Yoon

AU - Ahn, Jin Soo

AU - Yi, Young A.H.

AU - Chung, Shin Hye

AU - Yoo, Yeon Jee

AU - Ju, Sung Won

AU - Hwang, J. I.Yun

AU - Seo, Deog Gyu

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N2 - Objective. The purpose of this study was to compare the cytotoxicity of four denture adhesives on human gingival fibroblast cells. Materials and methods. Immortalized human gingival fibroblasts were cultured with one of four different denture adhesives, Polident, Protefix, Staydent or Denfix-A, which was placed in insert dishes (10% w/v concentration) for 48 h. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and flow cytometric apoptosis assay were used to evaluate cell viability and apoptosis rates. The fibroblasts were also examined under a scanning electron microscope. Results. The MTT assay showed that all denture adhesives resulted in a signi ficantly lower cell viability compared to the control cells propagated in normal culture medium (p < 0.05), with Staydent demonstrating the lowest cell viability. According to the flow cytometric apoptosis assay, Staydent and Protefix showed significantly higher apoptosis rates than the control group (p < 0.05), whereas Polident and Denfix-A did not demonstrate any significant differences (p > 0.05). Staydent showed the highest apoptosis rate. Scanning electron microscopy showed that the cells of the Staydent group underwent cytoplasmic membrane shrinkage, with cell free areas containing residual fragments of the membrane of dead cells. Conclusions. The four denture adhesives evaluated in this study imparted cytotoxic effects on human gingival fibroblast cells. Staydent showed the highest toxicity.

AB - Objective. The purpose of this study was to compare the cytotoxicity of four denture adhesives on human gingival fibroblast cells. Materials and methods. Immortalized human gingival fibroblasts were cultured with one of four different denture adhesives, Polident, Protefix, Staydent or Denfix-A, which was placed in insert dishes (10% w/v concentration) for 48 h. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and flow cytometric apoptosis assay were used to evaluate cell viability and apoptosis rates. The fibroblasts were also examined under a scanning electron microscope. Results. The MTT assay showed that all denture adhesives resulted in a signi ficantly lower cell viability compared to the control cells propagated in normal culture medium (p < 0.05), with Staydent demonstrating the lowest cell viability. According to the flow cytometric apoptosis assay, Staydent and Protefix showed significantly higher apoptosis rates than the control group (p < 0.05), whereas Polident and Denfix-A did not demonstrate any significant differences (p > 0.05). Staydent showed the highest apoptosis rate. Scanning electron microscopy showed that the cells of the Staydent group underwent cytoplasmic membrane shrinkage, with cell free areas containing residual fragments of the membrane of dead cells. Conclusions. The four denture adhesives evaluated in this study imparted cytotoxic effects on human gingival fibroblast cells. Staydent showed the highest toxicity.

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