Abstract
Objective. The purpose of this study was to compare the cytotoxicity of four denture adhesives on human gingival fibroblast cells. Materials and methods. Immortalized human gingival fibroblasts were cultured with one of four different denture adhesives, Polident, Protefix, Staydent or Denfix-A, which was placed in insert dishes (10% w/v concentration) for 48 h. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and flow cytometric apoptosis assay were used to evaluate cell viability and apoptosis rates. The fibroblasts were also examined under a scanning electron microscope. Results. The MTT assay showed that all denture adhesives resulted in a signi ficantly lower cell viability compared to the control cells propagated in normal culture medium (p < 0.05), with Staydent demonstrating the lowest cell viability. According to the flow cytometric apoptosis assay, Staydent and Protefix showed significantly higher apoptosis rates than the control group (p < 0.05), whereas Polident and Denfix-A did not demonstrate any significant differences (p > 0.05). Staydent showed the highest apoptosis rate. Scanning electron microscopy showed that the cells of the Staydent group underwent cytoplasmic membrane shrinkage, with cell free areas containing residual fragments of the membrane of dead cells. Conclusions. The four denture adhesives evaluated in this study imparted cytotoxic effects on human gingival fibroblast cells. Staydent showed the highest toxicity.
| Original language | English |
|---|---|
| Pages (from-to) | 87-92 |
| Number of pages | 6 |
| Journal | Acta odontologica Scandinavica |
| Volume | 73 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2014 Apr 1 |
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All Science Journal Classification (ASJC) codes
- Dentistry(all)
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Cytotoxicity of four denture adhesives on human gingival fibroblast cells. / Lee, Yoon; Ahn, Jin Soo; Yi, Young A.H.; Chung, Shin Hye; Yoo, Yeon Jee; Ju, Sung Won; Hwang, J. I.Yun; Seo, Deog Gyu.
In: Acta odontologica Scandinavica, Vol. 73, No. 2, 01.04.2014, p. 87-92.Research output: Contribution to journal › Article
TY - JOUR
T1 - Cytotoxicity of four denture adhesives on human gingival fibroblast cells
AU - Lee, Yoon
AU - Ahn, Jin Soo
AU - Yi, Young A.H.
AU - Chung, Shin Hye
AU - Yoo, Yeon Jee
AU - Ju, Sung Won
AU - Hwang, J. I.Yun
AU - Seo, Deog Gyu
PY - 2014/4/1
Y1 - 2014/4/1
N2 - Objective. The purpose of this study was to compare the cytotoxicity of four denture adhesives on human gingival fibroblast cells. Materials and methods. Immortalized human gingival fibroblasts were cultured with one of four different denture adhesives, Polident, Protefix, Staydent or Denfix-A, which was placed in insert dishes (10% w/v concentration) for 48 h. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and flow cytometric apoptosis assay were used to evaluate cell viability and apoptosis rates. The fibroblasts were also examined under a scanning electron microscope. Results. The MTT assay showed that all denture adhesives resulted in a signi ficantly lower cell viability compared to the control cells propagated in normal culture medium (p < 0.05), with Staydent demonstrating the lowest cell viability. According to the flow cytometric apoptosis assay, Staydent and Protefix showed significantly higher apoptosis rates than the control group (p < 0.05), whereas Polident and Denfix-A did not demonstrate any significant differences (p > 0.05). Staydent showed the highest apoptosis rate. Scanning electron microscopy showed that the cells of the Staydent group underwent cytoplasmic membrane shrinkage, with cell free areas containing residual fragments of the membrane of dead cells. Conclusions. The four denture adhesives evaluated in this study imparted cytotoxic effects on human gingival fibroblast cells. Staydent showed the highest toxicity.
AB - Objective. The purpose of this study was to compare the cytotoxicity of four denture adhesives on human gingival fibroblast cells. Materials and methods. Immortalized human gingival fibroblasts were cultured with one of four different denture adhesives, Polident, Protefix, Staydent or Denfix-A, which was placed in insert dishes (10% w/v concentration) for 48 h. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and flow cytometric apoptosis assay were used to evaluate cell viability and apoptosis rates. The fibroblasts were also examined under a scanning electron microscope. Results. The MTT assay showed that all denture adhesives resulted in a signi ficantly lower cell viability compared to the control cells propagated in normal culture medium (p < 0.05), with Staydent demonstrating the lowest cell viability. According to the flow cytometric apoptosis assay, Staydent and Protefix showed significantly higher apoptosis rates than the control group (p < 0.05), whereas Polident and Denfix-A did not demonstrate any significant differences (p > 0.05). Staydent showed the highest apoptosis rate. Scanning electron microscopy showed that the cells of the Staydent group underwent cytoplasmic membrane shrinkage, with cell free areas containing residual fragments of the membrane of dead cells. Conclusions. The four denture adhesives evaluated in this study imparted cytotoxic effects on human gingival fibroblast cells. Staydent showed the highest toxicity.
UR - http://www.scopus.com/inward/record.url?scp=84921466427&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84921466427&partnerID=8YFLogxK
U2 - 10.3109/00016357.2014.954266
DO - 10.3109/00016357.2014.954266
M3 - Article
C2 - 25220520
AN - SCOPUS:84921466427
VL - 73
SP - 87
EP - 92
JO - Acta Odontologica Scandinavica
JF - Acta Odontologica Scandinavica
SN - 0001-6357
IS - 2
ER -