Daidzein suppresses tumor necrosis factor-α induced migration and invasion by inhibiting hedgehog/gli1 signaling in human breast cancer cells

Cheng Bao, Hyeju Namgung, Jaehoo Lee, Hyun Chang Park, Jiwon Ko, Heejung Moon, Hyuk Wan Ko, Hong Jin Lee

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In breast cancer, the cytokine tumor necrosis factor-α (TNF-α) induces cell invasion, although the molecular basis of it has not been clearly elucidated. In this study, we investigated the role of daidzein in regulating TNF-α induced cell invasion and the underlying molecular mechanisms. Daidzein inhibited TNF-α induced cellular migration and invasion in estrogen receptor (ER) negative MCF10DCIS.com human breast cancer cells. TNF-α activated Hedgehog (Hh) signaling by enhancing Gli1 nuclear translocation and transcriptional activity, which resulted in increased invasiveness; these effects were blocked by daidzein and the Hh signaling inhibitors, cyclopamine and vismodegib. Moreover, these compounds suppressed TNF-α induced matrix metalloproteinase (MMP)-9 mRNA expression and activity. Taken together, mammary tumor cell invasiveness was stimulated by TNF-α induced activation of Hh signaling; these effects were abrogated by daidzein, which suppressed Gli1 activation, thereby inhibiting migration and invasion.

Original languageEnglish
Pages (from-to)3759-3767
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume62
Issue number17
DOIs
Publication statusPublished - 2014 Apr 30

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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