Daidzein suppresses tumor necrosis factor-α induced migration and invasion by inhibiting hedgehog/gli1 signaling in human breast cancer cells

Cheng Bao, Hyeju Namgung, Jaehoo Lee, Hyun Chang Park, Jiwon Ko, Heejung Moon, Hyuk Wan Ko, Hong Jin Lee

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In breast cancer, the cytokine tumor necrosis factor-α (TNF-α) induces cell invasion, although the molecular basis of it has not been clearly elucidated. In this study, we investigated the role of daidzein in regulating TNF-α induced cell invasion and the underlying molecular mechanisms. Daidzein inhibited TNF-α induced cellular migration and invasion in estrogen receptor (ER) negative MCF10DCIS.com human breast cancer cells. TNF-α activated Hedgehog (Hh) signaling by enhancing Gli1 nuclear translocation and transcriptional activity, which resulted in increased invasiveness; these effects were blocked by daidzein and the Hh signaling inhibitors, cyclopamine and vismodegib. Moreover, these compounds suppressed TNF-α induced matrix metalloproteinase (MMP)-9 mRNA expression and activity. Taken together, mammary tumor cell invasiveness was stimulated by TNF-α induced activation of Hh signaling; these effects were abrogated by daidzein, which suppressed Gli1 activation, thereby inhibiting migration and invasion.

Original languageEnglish
Pages (from-to)3759-3767
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume62
Issue number17
DOIs
Publication statusPublished - 2014 Apr 30

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Erinaceidae
daidzein
tumor necrosis factors
breast neoplasms
Tumor Necrosis Factor-alpha
Cells
Breast Neoplasms
cell invasion
HhAntag691
Chemical activation
gelatinase B
mammary neoplasms (animal)
Matrix Metalloproteinase 9
cell movement
Estrogen Receptors
neoplasm cells
Tumors
cytokines
Cytokines
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Bao, Cheng ; Namgung, Hyeju ; Lee, Jaehoo ; Park, Hyun Chang ; Ko, Jiwon ; Moon, Heejung ; Ko, Hyuk Wan ; Lee, Hong Jin. / Daidzein suppresses tumor necrosis factor-α induced migration and invasion by inhibiting hedgehog/gli1 signaling in human breast cancer cells. In: Journal of Agricultural and Food Chemistry. 2014 ; Vol. 62, No. 17. pp. 3759-3767.
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Daidzein suppresses tumor necrosis factor-α induced migration and invasion by inhibiting hedgehog/gli1 signaling in human breast cancer cells. / Bao, Cheng; Namgung, Hyeju; Lee, Jaehoo; Park, Hyun Chang; Ko, Jiwon; Moon, Heejung; Ko, Hyuk Wan; Lee, Hong Jin.

In: Journal of Agricultural and Food Chemistry, Vol. 62, No. 17, 30.04.2014, p. 3759-3767.

Research output: Contribution to journalArticle

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AU - Bao, Cheng

AU - Namgung, Hyeju

AU - Lee, Jaehoo

AU - Park, Hyun Chang

AU - Ko, Jiwon

AU - Moon, Heejung

AU - Ko, Hyuk Wan

AU - Lee, Hong Jin

PY - 2014/4/30

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N2 - In breast cancer, the cytokine tumor necrosis factor-α (TNF-α) induces cell invasion, although the molecular basis of it has not been clearly elucidated. In this study, we investigated the role of daidzein in regulating TNF-α induced cell invasion and the underlying molecular mechanisms. Daidzein inhibited TNF-α induced cellular migration and invasion in estrogen receptor (ER) negative MCF10DCIS.com human breast cancer cells. TNF-α activated Hedgehog (Hh) signaling by enhancing Gli1 nuclear translocation and transcriptional activity, which resulted in increased invasiveness; these effects were blocked by daidzein and the Hh signaling inhibitors, cyclopamine and vismodegib. Moreover, these compounds suppressed TNF-α induced matrix metalloproteinase (MMP)-9 mRNA expression and activity. Taken together, mammary tumor cell invasiveness was stimulated by TNF-α induced activation of Hh signaling; these effects were abrogated by daidzein, which suppressed Gli1 activation, thereby inhibiting migration and invasion.

AB - In breast cancer, the cytokine tumor necrosis factor-α (TNF-α) induces cell invasion, although the molecular basis of it has not been clearly elucidated. In this study, we investigated the role of daidzein in regulating TNF-α induced cell invasion and the underlying molecular mechanisms. Daidzein inhibited TNF-α induced cellular migration and invasion in estrogen receptor (ER) negative MCF10DCIS.com human breast cancer cells. TNF-α activated Hedgehog (Hh) signaling by enhancing Gli1 nuclear translocation and transcriptional activity, which resulted in increased invasiveness; these effects were blocked by daidzein and the Hh signaling inhibitors, cyclopamine and vismodegib. Moreover, these compounds suppressed TNF-α induced matrix metalloproteinase (MMP)-9 mRNA expression and activity. Taken together, mammary tumor cell invasiveness was stimulated by TNF-α induced activation of Hh signaling; these effects were abrogated by daidzein, which suppressed Gli1 activation, thereby inhibiting migration and invasion.

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