Dammarane triterpenes as potential SIRT1 activators from the leaves of Panax ginseng

Jun Li Yang; Thi Kim Quy Ha; Basanta Dhodary; Kuk Hwa Kim; Junsoo Park; Chul Ho Lee; Young Choong Kim; Won Keun Oh

doi:10.1021/np5002303

Dammarane triterpenes as potential SIRT1 activators from the leaves of Panax ginseng

Jun Li Yang, Thi Kim Quy Ha, Basanta Dhodary, Kuk Hwa Kim, Junsoo Park, Chul Ho Lee, Young Choong Kim, Won Keun Oh

Division of Bioscience and Biotechnology

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

During a search for SIRT1 activators originating in nature, three new dammarane triterpenes, 6α,20(S)-dihydroxydammar-3,12-dione-24-ene (1), 6α,20(S),24(S)-trihydroxydammar-3,12-dione-25-ene (2), and 6α,20(S),25-trihydroxydammar-3,12-dione-23-ene (3), as well as two known triterpenes, dammar-20(22),24-diene-3β,6α,12β-triol (4) and 20(S)-ginsenoside Rg₃ (5), were isolated from Panax ginseng leaves. Compounds 1 and 3-5 showed potential as SIRT1 activators, as analyzed by in vitro enzyme-based SIRT1-NAD/NADH and SIRT1-p53 luciferase cell-based assays. They were also found to increase the level of NAD⁺/NADH ratio in HEK293 cells. This study presents a new class of chemical entities that may be able to be developed as SIRT1 activators for antiaging and treatment of age-associated diseases.

Original language	English
Pages (from-to)	1615-1623
Number of pages	9
Journal	Journal of Natural Products
Volume	77
Issue number	7
DOIs	https://doi.org/10.1021/np5002303
Publication status	Published - 2014 Jul 25

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

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title = "Dammarane triterpenes as potential SIRT1 activators from the leaves of Panax ginseng",

abstract = "During a search for SIRT1 activators originating in nature, three new dammarane triterpenes, 6α,20(S)-dihydroxydammar-3,12-dione-24-ene (1), 6α,20(S),24(S)-trihydroxydammar-3,12-dione-25-ene (2), and 6α,20(S),25-trihydroxydammar-3,12-dione-23-ene (3), as well as two known triterpenes, dammar-20(22),24-diene-3β,6α,12β-triol (4) and 20(S)-ginsenoside Rg3 (5), were isolated from Panax ginseng leaves. Compounds 1 and 3-5 showed potential as SIRT1 activators, as analyzed by in vitro enzyme-based SIRT1-NAD/NADH and SIRT1-p53 luciferase cell-based assays. They were also found to increase the level of NAD+/NADH ratio in HEK293 cells. This study presents a new class of chemical entities that may be able to be developed as SIRT1 activators for antiaging and treatment of age-associated diseases.",

author = "Yang, {Jun Li} and Ha, {Thi Kim Quy} and Basanta Dhodary and Kim, {Kuk Hwa} and Junsoo Park and Lee, {Chul Ho} and Kim, {Young Choong} and Oh, {Won Keun}",

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AU - Yang, Jun Li

AU - Ha, Thi Kim Quy

AU - Dhodary, Basanta

AU - Kim, Kuk Hwa

AU - Park, Junsoo

AU - Lee, Chul Ho

AU - Kim, Young Choong

AU - Oh, Won Keun

PY - 2014/7/25

Y1 - 2014/7/25

N2 - During a search for SIRT1 activators originating in nature, three new dammarane triterpenes, 6α,20(S)-dihydroxydammar-3,12-dione-24-ene (1), 6α,20(S),24(S)-trihydroxydammar-3,12-dione-25-ene (2), and 6α,20(S),25-trihydroxydammar-3,12-dione-23-ene (3), as well as two known triterpenes, dammar-20(22),24-diene-3β,6α,12β-triol (4) and 20(S)-ginsenoside Rg3 (5), were isolated from Panax ginseng leaves. Compounds 1 and 3-5 showed potential as SIRT1 activators, as analyzed by in vitro enzyme-based SIRT1-NAD/NADH and SIRT1-p53 luciferase cell-based assays. They were also found to increase the level of NAD+/NADH ratio in HEK293 cells. This study presents a new class of chemical entities that may be able to be developed as SIRT1 activators for antiaging and treatment of age-associated diseases.

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Dammarane triterpenes as potential SIRT1 activators from the leaves of Panax ginseng

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