Abstract
This study examined the effect of sequential dilute acid hydrolysis and enzyme saccharification conditions of rice husk on hydrogen (H2) fermentation. The rice husk was hydrolyzed using dilute H2SO4 and then further enzymatically hydrolyzed with various concentrations (0.1, 0.25, 0.5, 0.75, and 1.0 mg protein mL−1) of Celluclast 1.5 L®. The sequential pretreatment significantly enhanced sugar recovery, up to 174% for 1.0 mg mL−1 concentration of the enzyme. Increasing the concentration of enzyme slightly increases sugar recovery. While the production of furfural and 5-hydroxymethylfurfural occurred during dilute acid hydrolysis, it was not observed during enzymatic saccharification. At the following H2 fermentation of the hydrolyzates, H2 yield was improved up to 150% by using the dilute acid and enzymatic hydrolyzate as substrate, as compared when only dilute acid hydrolyzate was used. The highest H2 yield of 473.1 mL H2 g−1 rice husk was achieved with enzyme concentration of 0.75 mg protein mL−1.
| Original language | English |
|---|---|
| Pages (from-to) | 27577-27583 |
| Number of pages | 7 |
| Journal | International Journal of Hydrogen Energy |
| Volume | 42 |
| Issue number | 45 |
| DOIs | |
| Publication status | Published - 2017 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Renewable Energy, Sustainability and the Environment
- Fuel Technology
- Condensed Matter Physics
- Energy Engineering and Power Technology
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Dark fermentative hydrogen production following the sequential dilute acid pretreatment and enzymatic saccharification of rice husk. / Gonzales, Ralph Rolly; Kim, Sang-Hyoun.
In: International Journal of Hydrogen Energy, Vol. 42, No. 45, 01.01.2017, p. 27577-27583.Research output: Contribution to journal › Article
TY - JOUR
T1 - Dark fermentative hydrogen production following the sequential dilute acid pretreatment and enzymatic saccharification of rice husk
AU - Gonzales, Ralph Rolly
AU - Kim, Sang-Hyoun
PY - 2017/1/1
Y1 - 2017/1/1
N2 - This study examined the effect of sequential dilute acid hydrolysis and enzyme saccharification conditions of rice husk on hydrogen (H2) fermentation. The rice husk was hydrolyzed using dilute H2SO4 and then further enzymatically hydrolyzed with various concentrations (0.1, 0.25, 0.5, 0.75, and 1.0 mg protein mL−1) of Celluclast 1.5 L®. The sequential pretreatment significantly enhanced sugar recovery, up to 174% for 1.0 mg mL−1 concentration of the enzyme. Increasing the concentration of enzyme slightly increases sugar recovery. While the production of furfural and 5-hydroxymethylfurfural occurred during dilute acid hydrolysis, it was not observed during enzymatic saccharification. At the following H2 fermentation of the hydrolyzates, H2 yield was improved up to 150% by using the dilute acid and enzymatic hydrolyzate as substrate, as compared when only dilute acid hydrolyzate was used. The highest H2 yield of 473.1 mL H2 g−1 rice husk was achieved with enzyme concentration of 0.75 mg protein mL−1.
AB - This study examined the effect of sequential dilute acid hydrolysis and enzyme saccharification conditions of rice husk on hydrogen (H2) fermentation. The rice husk was hydrolyzed using dilute H2SO4 and then further enzymatically hydrolyzed with various concentrations (0.1, 0.25, 0.5, 0.75, and 1.0 mg protein mL−1) of Celluclast 1.5 L®. The sequential pretreatment significantly enhanced sugar recovery, up to 174% for 1.0 mg mL−1 concentration of the enzyme. Increasing the concentration of enzyme slightly increases sugar recovery. While the production of furfural and 5-hydroxymethylfurfural occurred during dilute acid hydrolysis, it was not observed during enzymatic saccharification. At the following H2 fermentation of the hydrolyzates, H2 yield was improved up to 150% by using the dilute acid and enzymatic hydrolyzate as substrate, as compared when only dilute acid hydrolyzate was used. The highest H2 yield of 473.1 mL H2 g−1 rice husk was achieved with enzyme concentration of 0.75 mg protein mL−1.
UR - http://www.scopus.com/inward/record.url?scp=85029514424&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029514424&partnerID=8YFLogxK
U2 - 10.1016/j.ijhydene.2017.08.185
DO - 10.1016/j.ijhydene.2017.08.185
M3 - Article
AN - SCOPUS:85029514424
VL - 42
SP - 27577
EP - 27583
JO - International Journal of Hydrogen Energy
JF - International Journal of Hydrogen Energy
SN - 0360-3199
IS - 45
ER -