Abstract
This study examined the effect of sequential dilute acid hydrolysis and enzyme saccharification conditions of rice husk on hydrogen (H2) fermentation. The rice husk was hydrolyzed using dilute H2SO4 and then further enzymatically hydrolyzed with various concentrations (0.1, 0.25, 0.5, 0.75, and 1.0 mg protein mL−1) of Celluclast 1.5 L®. The sequential pretreatment significantly enhanced sugar recovery, up to 174% for 1.0 mg mL−1 concentration of the enzyme. Increasing the concentration of enzyme slightly increases sugar recovery. While the production of furfural and 5-hydroxymethylfurfural occurred during dilute acid hydrolysis, it was not observed during enzymatic saccharification. At the following H2 fermentation of the hydrolyzates, H2 yield was improved up to 150% by using the dilute acid and enzymatic hydrolyzate as substrate, as compared when only dilute acid hydrolyzate was used. The highest H2 yield of 473.1 mL H2 g−1 rice husk was achieved with enzyme concentration of 0.75 mg protein mL−1.
Original language | English |
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Pages (from-to) | 27577-27583 |
Number of pages | 7 |
Journal | International Journal of Hydrogen Energy |
Volume | 42 |
Issue number | 45 |
DOIs | |
Publication status | Published - 2017 |
Bibliographical note
Funding Information:This research was supported by the Daegu University Grant, 2016 .
Publisher Copyright:
© 2017 Hydrogen Energy Publications LLC
All Science Journal Classification (ASJC) codes
- Renewable Energy, Sustainability and the Environment
- Fuel Technology
- Condensed Matter Physics
- Energy Engineering and Power Technology