De novo formation of basal bodies in Naegleria gruberi: Regulation by phosphorylation

Hong Kyung Kim, Jeong Gu Kang, Shigehiko Yumura, Charles J. Walsh, Won Cho Jin, Joohun Lee

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The de novo formation of basal bodies in Naegleria gruberi was preceded by the transient formation of a microtubule (MT)-nucleating complex containing γ-tubulin, pericentrin, and myosin II complex (GPM complex). The MT-nucleating activity of GPM complexes was maximal just before the formation of visible basal bodies and then rapidly decreased. The regulation of MT-nucleating activity of GPM complexes was accomplished by a transient phosphorylation of the complex. Inhibition of dephosphorylation after the formation of basal bodies resulted in the formation of multiple flagella. 2D-gel electrophoresis and Western blotting showed a parallel relationship between the MT-nucleating activity of GPM complexes and the presence of hyperphosphorylated γ-tubulin in the complexes. These data suggest that the nucleation of MTs by GPM complexes precedes the de novo formation of basal bodies and that the regulation of MT-nucleating activity of GPM complexes is essential to the regulation of basal body number.

Original languageEnglish
Pages (from-to)719-724
Number of pages6
JournalJournal of Cell Biology
Volume169
Issue number5
DOIs
Publication statusPublished - 2005 Jun

All Science Journal Classification (ASJC) codes

  • Cell Biology

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