Decellularized human periodontal ligament for periodontium regeneration

Hyoju Son, Mijeong Jeon, Hyung Jun Choi, Hyo Seol Lee, Ik Hwan Kim, Chung Min Kang, JeSeon Song

Research output: Contribution to journalArticle

Abstract

Regenerating the periodontal ligament (PDL) is a crucial factor for periodontal tissue regeneration in the presence of traumatized and periodontally damaged teeth. Various methods have been applied for periodontal regeneration, including tissue substitutes, bioactive materials, and synthetic scaffolds. However, all of these treatments have had limited success in structural and functional periodontal tissue regeneration. To achieve the goal of complete periodontal regeneration, many studies have evaluated the effectiveness of decellularized scaffolds fabricated via tissue engineering. The aim of this study was to fabricate a decellularized periodontal scaffold of human tooth slices and determine its regeneration potential. We evaluated two different protocols applied to tooth slices obtained from human healthy third molars. The extracellular matrix scaffold decellularized using sodium dodecyl sulfate and Triton X-100, which are effective in removing nuclear components, was demonstrated to preserve an intact structure and composition. Furthermore, the decellularized scaffold could support repopulation of PDL stem cells near the cementum and expressed cementum and periodontal-ligament-related genes. These results show that decellularized PDL scaffolds of human teeth are capable of inducing the proliferation and differentiation of mesenchymal stem cells, thus having regeneration potential for use in future periodontal regenerative tissue engineering.

Original languageEnglish
Article numbere0221236
JournalPloS one
Volume14
Issue number8
DOIs
Publication statusPublished - 2019 Jan 1

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Periodontium
Periodontal Ligament
Ligaments
ligaments
Scaffolds
Regeneration
mouth
teeth
tissue repair
Tissue regeneration
tissue engineering
Tooth
stem cells
Dental Cementum
Stem cells
Tissue engineering
Tissue Engineering
sodium dodecyl sulfate
extracellular matrix
Bioelectric potentials

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Son, H., Jeon, M., Choi, H. J., Lee, H. S., Kim, I. H., Kang, C. M., & Song, J. (2019). Decellularized human periodontal ligament for periodontium regeneration. PloS one, 14(8), [e0221236]. https://doi.org/10.1371/journal.pone.0221236
Son, Hyoju ; Jeon, Mijeong ; Choi, Hyung Jun ; Lee, Hyo Seol ; Kim, Ik Hwan ; Kang, Chung Min ; Song, JeSeon. / Decellularized human periodontal ligament for periodontium regeneration. In: PloS one. 2019 ; Vol. 14, No. 8.
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Son, H, Jeon, M, Choi, HJ, Lee, HS, Kim, IH, Kang, CM & Song, J 2019, 'Decellularized human periodontal ligament for periodontium regeneration', PloS one, vol. 14, no. 8, e0221236. https://doi.org/10.1371/journal.pone.0221236

Decellularized human periodontal ligament for periodontium regeneration. / Son, Hyoju; Jeon, Mijeong; Choi, Hyung Jun; Lee, Hyo Seol; Kim, Ik Hwan; Kang, Chung Min; Song, JeSeon.

In: PloS one, Vol. 14, No. 8, e0221236, 01.01.2019.

Research output: Contribution to journalArticle

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Son H, Jeon M, Choi HJ, Lee HS, Kim IH, Kang CM et al. Decellularized human periodontal ligament for periodontium regeneration. PloS one. 2019 Jan 1;14(8). e0221236. https://doi.org/10.1371/journal.pone.0221236