Deconstructing Adipogenesis Induced by β3-Adrenergic Receptor Activation with Single-Cell Expression Profiling

Rayanne B. Burl, Vanesa D. Ramseyer, Elizabeth A. Rondini, Roger Pique-Regi, Yun Hee Lee, James G. Granneman

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Recruitment of brown/beige adipocytes (BAs) in white adipose tissue (WAT) involves proliferation and differentiation of adipocyte stem cells (ASCs) in concert with close interactions with resident immune cells. To deconvolve stromal cell heterogeneity in a comprehensive and unbiased fashion, we performed single-cell RNA sequencing (scRNA-seq) of >33,000 stromal/vascular cells from epididymal WAT (eWAT) and inguinal WAT (iWAT) under control conditions and during β3-adrenergic receptor (ADRB3) activation. scRNA-seq identified distinct ASC subpopulations in eWAT and iWAT that appeared to be differentially poised to enter the adipogenic pathway. ADRB3 activation triggered the dramatic appearance of proliferating ASCs in eWAT, whose differentiation into BAs could be inferred from a single time point. scRNA-seq identified various immune cell types in eWAT, including a proliferating macrophage subpopulation that occupies adipogenic niches. These results demonstrate the power of scRNA-seq to deconstruct adipogenic niches and suggest novel functional interactions among resident stromal cell subpopulations. Burl et al. employ single-cell RNA sequencing (scRNA-seq) of mouse adipose tissue to identify distinct subpopulations of adipocyte progenitors and immune cells. In a model of in vivo brown adipogenesis, scRNA-seq data are used to deconstruct adipogenic niches, map differentiation trajectories, and suggest novel functional interactions among resident stromal cell subpopulations.

Original languageEnglish
Pages (from-to)300-309.e4
JournalCell Metabolism
Volume28
Issue number2
DOIs
Publication statusPublished - 2018 Aug 7

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Adipogenesis
RNA Sequence Analysis
Adrenergic Receptors
Stromal Cells
Adipocytes
White Adipose Tissue
Stem Cells
Groin
Brown Adipocytes
Blood Vessels
Adipose Tissue
Macrophages

All Science Journal Classification (ASJC) codes

  • Physiology
  • Molecular Biology
  • Cell Biology

Cite this

Burl, R. B., Ramseyer, V. D., Rondini, E. A., Pique-Regi, R., Lee, Y. H., & Granneman, J. G. (2018). Deconstructing Adipogenesis Induced by β3-Adrenergic Receptor Activation with Single-Cell Expression Profiling. Cell Metabolism, 28(2), 300-309.e4. https://doi.org/10.1016/j.cmet.2018.05.025
Burl, Rayanne B. ; Ramseyer, Vanesa D. ; Rondini, Elizabeth A. ; Pique-Regi, Roger ; Lee, Yun Hee ; Granneman, James G. / Deconstructing Adipogenesis Induced by β3-Adrenergic Receptor Activation with Single-Cell Expression Profiling. In: Cell Metabolism. 2018 ; Vol. 28, No. 2. pp. 300-309.e4.
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Burl, RB, Ramseyer, VD, Rondini, EA, Pique-Regi, R, Lee, YH & Granneman, JG 2018, 'Deconstructing Adipogenesis Induced by β3-Adrenergic Receptor Activation with Single-Cell Expression Profiling', Cell Metabolism, vol. 28, no. 2, pp. 300-309.e4. https://doi.org/10.1016/j.cmet.2018.05.025

Deconstructing Adipogenesis Induced by β3-Adrenergic Receptor Activation with Single-Cell Expression Profiling. / Burl, Rayanne B.; Ramseyer, Vanesa D.; Rondini, Elizabeth A.; Pique-Regi, Roger; Lee, Yun Hee; Granneman, James G.

In: Cell Metabolism, Vol. 28, No. 2, 07.08.2018, p. 300-309.e4.

Research output: Contribution to journalArticle

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