Decreased bone volume and bone mineral density in the tibial trabecular bone is associated with Per2 gene by 405 nm laser stimulation

Yeong Min Yoo, Myung Han Lee, Ji Hyung Park, Dong Hyun Seo, Sangyeob Lee, Byungjo Jung, Han Sung Kim, Kiho Bae

Research output: Contribution to journalArticle

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Abstract

Low-level laser therapy/treatment (LLLT) using a minimally invasive laser needle system (MILNS) might enhance bone formation and suppress bone resorption. In this study, the use of 405 nm LLLT led to decreases in bone volume and bone mineral density (BMD) of tibial trabecular bone in wild-type (WT) and Per2 knockout (KO) mice. Bone volume and bone mineral density of tibial trabecular bone was decreased by 405 nm LLLT in Per2 KO compared to WT mice at two and four weeks. To determine the reduction in tibial bone, mRNA expressions of alkaline phosphatase (ALP) and Per2 were investigated at four weeks after 405 nm laser stimulation using MILNS. ALP gene expression was significantly reduced in the LLLT-stimulated right tibial bone of WT and Per2 KO mice compared to the non-irradiated left tibia (p < 0.001). Per2 mRNA expression in WT mice was significantly reduced in the LLLT-stimulated right tibial bone compared to the non-irradiated left tibia (p < 0.001). To identify the decrease in tibial bone mediated by the Per2 gene, levels of runt-related transcription factor 2 (Runx2) and ALP mRNAs were determined in non-irradiated WT and Per2 KO mice. These results demonstrated significant downregulation of Runx2 and ALPmRNA levels in Per2 KO mice (p < 0.001). Therefore, the reduction in tibial trabecular bone resulting from 405 nm LLLT using MILNS might be associated with Per2 gene expression.

Original languageEnglish
Pages (from-to)27401-27410
Number of pages10
JournalInternational journal of molecular sciences
Volume16
Issue number11
DOIs
Publication statusPublished - 2015 Nov 16

Fingerprint

stimulation
genes
Bone Density
bones
Minerals
Bone
Lasers
Genes
minerals
Bone and Bones
Knockout Mice
knockout mice
Needles
lasers
Alkaline Phosphatase
therapy
Tibia
Messenger RNA
phosphatases
Therapeutics

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Yoo, Yeong Min ; Lee, Myung Han ; Park, Ji Hyung ; Seo, Dong Hyun ; Lee, Sangyeob ; Jung, Byungjo ; Kim, Han Sung ; Bae, Kiho. / Decreased bone volume and bone mineral density in the tibial trabecular bone is associated with Per2 gene by 405 nm laser stimulation. In: International journal of molecular sciences. 2015 ; Vol. 16, No. 11. pp. 27401-27410.
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title = "Decreased bone volume and bone mineral density in the tibial trabecular bone is associated with Per2 gene by 405 nm laser stimulation",
abstract = "Low-level laser therapy/treatment (LLLT) using a minimally invasive laser needle system (MILNS) might enhance bone formation and suppress bone resorption. In this study, the use of 405 nm LLLT led to decreases in bone volume and bone mineral density (BMD) of tibial trabecular bone in wild-type (WT) and Per2 knockout (KO) mice. Bone volume and bone mineral density of tibial trabecular bone was decreased by 405 nm LLLT in Per2 KO compared to WT mice at two and four weeks. To determine the reduction in tibial bone, mRNA expressions of alkaline phosphatase (ALP) and Per2 were investigated at four weeks after 405 nm laser stimulation using MILNS. ALP gene expression was significantly reduced in the LLLT-stimulated right tibial bone of WT and Per2 KO mice compared to the non-irradiated left tibia (p < 0.001). Per2 mRNA expression in WT mice was significantly reduced in the LLLT-stimulated right tibial bone compared to the non-irradiated left tibia (p < 0.001). To identify the decrease in tibial bone mediated by the Per2 gene, levels of runt-related transcription factor 2 (Runx2) and ALP mRNAs were determined in non-irradiated WT and Per2 KO mice. These results demonstrated significant downregulation of Runx2 and ALPmRNA levels in Per2 KO mice (p < 0.001). Therefore, the reduction in tibial trabecular bone resulting from 405 nm LLLT using MILNS might be associated with Per2 gene expression.",
author = "Yoo, {Yeong Min} and Lee, {Myung Han} and Park, {Ji Hyung} and Seo, {Dong Hyun} and Sangyeob Lee and Byungjo Jung and Kim, {Han Sung} and Kiho Bae",
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Decreased bone volume and bone mineral density in the tibial trabecular bone is associated with Per2 gene by 405 nm laser stimulation. / Yoo, Yeong Min; Lee, Myung Han; Park, Ji Hyung; Seo, Dong Hyun; Lee, Sangyeob; Jung, Byungjo; Kim, Han Sung; Bae, Kiho.

In: International journal of molecular sciences, Vol. 16, No. 11, 16.11.2015, p. 27401-27410.

Research output: Contribution to journalArticle

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AU - Yoo, Yeong Min

AU - Lee, Myung Han

AU - Park, Ji Hyung

AU - Seo, Dong Hyun

AU - Lee, Sangyeob

AU - Jung, Byungjo

AU - Kim, Han Sung

AU - Bae, Kiho

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N2 - Low-level laser therapy/treatment (LLLT) using a minimally invasive laser needle system (MILNS) might enhance bone formation and suppress bone resorption. In this study, the use of 405 nm LLLT led to decreases in bone volume and bone mineral density (BMD) of tibial trabecular bone in wild-type (WT) and Per2 knockout (KO) mice. Bone volume and bone mineral density of tibial trabecular bone was decreased by 405 nm LLLT in Per2 KO compared to WT mice at two and four weeks. To determine the reduction in tibial bone, mRNA expressions of alkaline phosphatase (ALP) and Per2 were investigated at four weeks after 405 nm laser stimulation using MILNS. ALP gene expression was significantly reduced in the LLLT-stimulated right tibial bone of WT and Per2 KO mice compared to the non-irradiated left tibia (p < 0.001). Per2 mRNA expression in WT mice was significantly reduced in the LLLT-stimulated right tibial bone compared to the non-irradiated left tibia (p < 0.001). To identify the decrease in tibial bone mediated by the Per2 gene, levels of runt-related transcription factor 2 (Runx2) and ALP mRNAs were determined in non-irradiated WT and Per2 KO mice. These results demonstrated significant downregulation of Runx2 and ALPmRNA levels in Per2 KO mice (p < 0.001). Therefore, the reduction in tibial trabecular bone resulting from 405 nm LLLT using MILNS might be associated with Per2 gene expression.

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