Decreased circulating klotho levels in patients undergoing dialysis and relationship to oxidative stress and inflammation

Hyung Jung Oh, Bo Young Nam, Mi Jung Lee, Chan Ho Kim, Hyang Mo Koo, Fa Mee Doh, Jae Hyun Han, Eun Jin Kim, Ji Suk Han, Jung Tak Park, TaeHyun Yoo, Shin-Wook Kang, Dae Suk Han, SeungHyeok Han

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Introduction: It has been reported that klotho deficiency is associated with oxidative stress and inflammation in experimental kidney disease models. Patients with end-stage renal disease (ESRD) are particularly characterized by increased oxidative stress and inflammation. However, little is known about the relationship between these features and klotho in patients with ESRD. ♦ Methods: We conducted a single-center, cross-sectional study of 78 patients receiving peritoneal dialysis (PD). Serum concentrations of klotho, high-sensitivity C-reactive protein (hsCRP), interleukin-6 (IL-6), and 8-isoprostane were measured by enzyme-linked immunosorbent assay. To define factors independently associated with klotho, we determined Spearman’s correlation coefficients for between co-variates and conducted multiple linear regression analyses. ♦ Results: Patients were classified by median concentration of klotho. In patients with klotho levels > 329.6 pg/mL, serum 8-isoprostane and IL-6 levels were significantly higher than in those with klotho levels < 329.6 pg/mL. In correlation analyses, log 8-isoprostane (γ = –0.310, p = 0.006) and log IL-6 (γ = –0.343, p = 0.002) were inversely correlated with log klotho. After adjustment for age, gender, mean arterial pressure, log intact parathyroid hormone, and log IL-6, log 8-isoprostane was independently associated with log klotho (β = –0.158, p = 0.040). However, the significant relationship between klotho and IL-6 was not seen in an adjusted model. ♦ Conclusions: This study showed that circulating klotho levels were significantly associated with 8-isoprostane levels in patients undergoing PD, suggesting a potential link between klotho deficiency and enhanced oxidative stress in ESRD patients.

Original languageEnglish
Pages (from-to)43-51
Number of pages9
JournalPeritoneal Dialysis International
Volume35
Issue number1
DOIs
Publication statusPublished - 2015 Jan 1

Fingerprint

8-epi-prostaglandin F2alpha
Dialysis
Oxidative Stress
Inflammation
Interleukin-6
Chronic Kidney Failure
Peritoneal Dialysis
Kidney Diseases
Parathyroid Hormone
Serum
Interleukin-8
C-Reactive Protein
Linear Models
Arterial Pressure
Cross-Sectional Studies
Enzyme-Linked Immunosorbent Assay
Regression Analysis

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

Oh, Hyung Jung ; Nam, Bo Young ; Lee, Mi Jung ; Kim, Chan Ho ; Koo, Hyang Mo ; Doh, Fa Mee ; Han, Jae Hyun ; Kim, Eun Jin ; Han, Ji Suk ; Park, Jung Tak ; Yoo, TaeHyun ; Kang, Shin-Wook ; Han, Dae Suk ; Han, SeungHyeok. / Decreased circulating klotho levels in patients undergoing dialysis and relationship to oxidative stress and inflammation. In: Peritoneal Dialysis International. 2015 ; Vol. 35, No. 1. pp. 43-51.
@article{ced740806585430395898120fcbaf85e,
title = "Decreased circulating klotho levels in patients undergoing dialysis and relationship to oxidative stress and inflammation",
abstract = "Introduction: It has been reported that klotho deficiency is associated with oxidative stress and inflammation in experimental kidney disease models. Patients with end-stage renal disease (ESRD) are particularly characterized by increased oxidative stress and inflammation. However, little is known about the relationship between these features and klotho in patients with ESRD. ♦ Methods: We conducted a single-center, cross-sectional study of 78 patients receiving peritoneal dialysis (PD). Serum concentrations of klotho, high-sensitivity C-reactive protein (hsCRP), interleukin-6 (IL-6), and 8-isoprostane were measured by enzyme-linked immunosorbent assay. To define factors independently associated with klotho, we determined Spearman’s correlation coefficients for between co-variates and conducted multiple linear regression analyses. ♦ Results: Patients were classified by median concentration of klotho. In patients with klotho levels > 329.6 pg/mL, serum 8-isoprostane and IL-6 levels were significantly higher than in those with klotho levels < 329.6 pg/mL. In correlation analyses, log 8-isoprostane (γ = –0.310, p = 0.006) and log IL-6 (γ = –0.343, p = 0.002) were inversely correlated with log klotho. After adjustment for age, gender, mean arterial pressure, log intact parathyroid hormone, and log IL-6, log 8-isoprostane was independently associated with log klotho (β = –0.158, p = 0.040). However, the significant relationship between klotho and IL-6 was not seen in an adjusted model. ♦ Conclusions: This study showed that circulating klotho levels were significantly associated with 8-isoprostane levels in patients undergoing PD, suggesting a potential link between klotho deficiency and enhanced oxidative stress in ESRD patients.",
author = "Oh, {Hyung Jung} and Nam, {Bo Young} and Lee, {Mi Jung} and Kim, {Chan Ho} and Koo, {Hyang Mo} and Doh, {Fa Mee} and Han, {Jae Hyun} and Kim, {Eun Jin} and Han, {Ji Suk} and Park, {Jung Tak} and TaeHyun Yoo and Shin-Wook Kang and Han, {Dae Suk} and SeungHyeok Han",
year = "2015",
month = "1",
day = "1",
doi = "10.3747/pdi.2013.00150",
language = "English",
volume = "35",
pages = "43--51",
journal = "Peritoneal Dialysis International",
issn = "0896-8608",
publisher = "Multimed Inc.",
number = "1",

}

Decreased circulating klotho levels in patients undergoing dialysis and relationship to oxidative stress and inflammation. / Oh, Hyung Jung; Nam, Bo Young; Lee, Mi Jung; Kim, Chan Ho; Koo, Hyang Mo; Doh, Fa Mee; Han, Jae Hyun; Kim, Eun Jin; Han, Ji Suk; Park, Jung Tak; Yoo, TaeHyun; Kang, Shin-Wook; Han, Dae Suk; Han, SeungHyeok.

In: Peritoneal Dialysis International, Vol. 35, No. 1, 01.01.2015, p. 43-51.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Decreased circulating klotho levels in patients undergoing dialysis and relationship to oxidative stress and inflammation

AU - Oh, Hyung Jung

AU - Nam, Bo Young

AU - Lee, Mi Jung

AU - Kim, Chan Ho

AU - Koo, Hyang Mo

AU - Doh, Fa Mee

AU - Han, Jae Hyun

AU - Kim, Eun Jin

AU - Han, Ji Suk

AU - Park, Jung Tak

AU - Yoo, TaeHyun

AU - Kang, Shin-Wook

AU - Han, Dae Suk

AU - Han, SeungHyeok

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Introduction: It has been reported that klotho deficiency is associated with oxidative stress and inflammation in experimental kidney disease models. Patients with end-stage renal disease (ESRD) are particularly characterized by increased oxidative stress and inflammation. However, little is known about the relationship between these features and klotho in patients with ESRD. ♦ Methods: We conducted a single-center, cross-sectional study of 78 patients receiving peritoneal dialysis (PD). Serum concentrations of klotho, high-sensitivity C-reactive protein (hsCRP), interleukin-6 (IL-6), and 8-isoprostane were measured by enzyme-linked immunosorbent assay. To define factors independently associated with klotho, we determined Spearman’s correlation coefficients for between co-variates and conducted multiple linear regression analyses. ♦ Results: Patients were classified by median concentration of klotho. In patients with klotho levels > 329.6 pg/mL, serum 8-isoprostane and IL-6 levels were significantly higher than in those with klotho levels < 329.6 pg/mL. In correlation analyses, log 8-isoprostane (γ = –0.310, p = 0.006) and log IL-6 (γ = –0.343, p = 0.002) were inversely correlated with log klotho. After adjustment for age, gender, mean arterial pressure, log intact parathyroid hormone, and log IL-6, log 8-isoprostane was independently associated with log klotho (β = –0.158, p = 0.040). However, the significant relationship between klotho and IL-6 was not seen in an adjusted model. ♦ Conclusions: This study showed that circulating klotho levels were significantly associated with 8-isoprostane levels in patients undergoing PD, suggesting a potential link between klotho deficiency and enhanced oxidative stress in ESRD patients.

AB - Introduction: It has been reported that klotho deficiency is associated with oxidative stress and inflammation in experimental kidney disease models. Patients with end-stage renal disease (ESRD) are particularly characterized by increased oxidative stress and inflammation. However, little is known about the relationship between these features and klotho in patients with ESRD. ♦ Methods: We conducted a single-center, cross-sectional study of 78 patients receiving peritoneal dialysis (PD). Serum concentrations of klotho, high-sensitivity C-reactive protein (hsCRP), interleukin-6 (IL-6), and 8-isoprostane were measured by enzyme-linked immunosorbent assay. To define factors independently associated with klotho, we determined Spearman’s correlation coefficients for between co-variates and conducted multiple linear regression analyses. ♦ Results: Patients were classified by median concentration of klotho. In patients with klotho levels > 329.6 pg/mL, serum 8-isoprostane and IL-6 levels were significantly higher than in those with klotho levels < 329.6 pg/mL. In correlation analyses, log 8-isoprostane (γ = –0.310, p = 0.006) and log IL-6 (γ = –0.343, p = 0.002) were inversely correlated with log klotho. After adjustment for age, gender, mean arterial pressure, log intact parathyroid hormone, and log IL-6, log 8-isoprostane was independently associated with log klotho (β = –0.158, p = 0.040). However, the significant relationship between klotho and IL-6 was not seen in an adjusted model. ♦ Conclusions: This study showed that circulating klotho levels were significantly associated with 8-isoprostane levels in patients undergoing PD, suggesting a potential link between klotho deficiency and enhanced oxidative stress in ESRD patients.

UR - http://www.scopus.com/inward/record.url?scp=84928476747&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928476747&partnerID=8YFLogxK

U2 - 10.3747/pdi.2013.00150

DO - 10.3747/pdi.2013.00150

M3 - Article

VL - 35

SP - 43

EP - 51

JO - Peritoneal Dialysis International

JF - Peritoneal Dialysis International

SN - 0896-8608

IS - 1

ER -