Deferoxamine reduces inflammation and osteoclastogenesis in avulsed teeth

Ko Eun Lee, Mijeong Jeon, Seunghan Mo, Hyo Seol Lee, JeSeon Song, Hyung Jun Choi, Hyunsoo Cho, Chung Min Kang

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Replacement and inflammatory resorption are serious complications associated with the delayed replantation of avulsed teeth. In this study, we aimed to assess whether deferoxamine (DFO) can suppress inflammation and osteoclastogenesis in vitro and attenuate inflammation and bone resorption in a replanted rat tooth model. Cell viability and inflammation were evaluated in RAW264.7 cells. Osteoclastogenesis was confirmed by tartrate‐resistant acid phosphatase staining, reactive oxygen species (ROS) measurement, and quantitative reverse transcriptase–polymerase chain reaction in teeth exposed to different concentrations of DFO. In vivo, molars of 31 six‐weekold male Sprague–Dawley rats were extracted and stored in saline (n = 10) or DFO solution (n = 21) before replantation. Micro‐computed tomography (micro‐CT) imaging and histological analysis were performed to evaluate inflammation and root and alveolar bone resorption. DFO downregulated the genes related to inflammation and osteoclastogenesis. DFO also reduced ROS production and regulated specific pathways. Furthermore, the results of the micro‐CT and histological analyses provided evidence of the decrease in inflammation and hard tissue resorption in the DFO group. Overall, these results suggest that DFO reduces inflammation and osteoclastogenesis in a tooth replantation model, and thus, it has to be further investigated as a root surface treatment option for an avulsed tooth.

Original languageEnglish
Article number8225
JournalInternational journal of molecular sciences
Volume22
Issue number15
DOIs
Publication statusPublished - 2021 Aug 1

Bibliographical note

Funding Information:
Funding: This research was funded by the Yonsei University College of Dentistry Fund (6‐2019‐ 0006).

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Fingerprint

Dive into the research topics of 'Deferoxamine reduces inflammation and osteoclastogenesis in avulsed teeth'. Together they form a unique fingerprint.

Cite this