Deficiency of Caenorhabditis elegans RecQ5 homologue reduces life span and increases sensitivity to ionizing radiation

Yun Seong Jeong, Yool Le Kang, Ki Ho Lim, Myon Hee Lee, Jiyeung Lee, Hyeon-Sook Koo

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Gene expression and RNA interference phenotypes were investigated for a Caenorhabditis elegans homologue (Ce-RCQ-5) of human RecQ5 protein. Expression of the mRNA was observed by in situ hybridization from earliest embryogenesis and gradually decreased during late embryogenesis. Ce-RCQ-5 was immuno-localized in the nuclei of embryos, germ cells, and oocytes and also in the nuclei of various somatic cells of larvae and adults. Despite ubiquitous expression in postembryonic cells, RCQ-5 protein expression was highest in intestinal cells, which was confirmed by tagging the gene expression with green fluorescence protein. When endogenous Ce-rcq-5 gene expression was inhibited by RNA interference, no clear phenotypes were observed during development. However, C. elegans life span was reduced by 37% due to RNA interference of rcq-5 gene, suggesting its possible role in maintenance of genomic stability, as has been ascribed to other RecQ family DNA helicases. In addition, C. elegans became significantly more sensitive to ionizing radiation after inhibition of rcq-5 gene expression, indicating an involvement of C. elegans RCQ-5 in a cellular response to DNA damage, possibly in DNA repair.

Original languageEnglish
Pages (from-to)1309-1319
Number of pages11
JournalDNA Repair
Volume2
Issue number12
DOIs
Publication statusPublished - 2003 Dec 9

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Ionizing radiation
Caenorhabditis elegans
Ionizing Radiation
Gene expression
RNA Interference
Gene Expression
RNA
Embryonic Development
RecQ Helicases
Phenotype
Genomic Instability
DNA
Germ Cells
DNA Repair
DNA Damage
Oocytes
In Situ Hybridization
Larva
Proteins
Repair

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Jeong, Yun Seong ; Kang, Yool Le ; Lim, Ki Ho ; Lee, Myon Hee ; Lee, Jiyeung ; Koo, Hyeon-Sook. / Deficiency of Caenorhabditis elegans RecQ5 homologue reduces life span and increases sensitivity to ionizing radiation. In: DNA Repair. 2003 ; Vol. 2, No. 12. pp. 1309-1319.
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Deficiency of Caenorhabditis elegans RecQ5 homologue reduces life span and increases sensitivity to ionizing radiation. / Jeong, Yun Seong; Kang, Yool Le; Lim, Ki Ho; Lee, Myon Hee; Lee, Jiyeung; Koo, Hyeon-Sook.

In: DNA Repair, Vol. 2, No. 12, 09.12.2003, p. 1309-1319.

Research output: Contribution to journalArticle

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