Deformation of the cell nucleus under indentation

Mechanics and mechanisms

A. Vaziri, Hyungsuk Lee, M. R. Kaazempur Mofrad

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Computational models of the cell nucleus, along with experimental observations, can help in understanding the biomechanics of force-induced nuclear deformation and mechanisms of stress transition throughout the nucleus. Here, we develop a computational model for an isolated nucleus undergoing indentation, which includes separate components representing the nucleoplasm and the nuclear envelope. The nuclear envelope itself is composed of three separate layers: two thin elastic layers representing the inner and outer nuclear membranes and one thicker layer representing the nuclear lamina. The proposed model is capable of separating the structural role of major nuclear components in the force-induced biological response of the nucleus (and ultimately the cell). A systematic analysis is carried out to explore the role of major individual nuclear elements, namely inner and outer membranes, nuclear lamina, and nucleoplasm, as well as the loading and experimental factors such as indentation rate and probe angle, on the biomechanical response of an isolated nucleus in atomic force microscopy indentation experiment.

Original languageEnglish
Pages (from-to)2126-2135
Number of pages10
JournalJournal of Materials Research
Volume21
Issue number8
DOIs
Publication statusPublished - 2006 Aug 1

Fingerprint

indentation
Indentation
Mechanics
Cells
nuclei
Membranes
Biomechanics
envelopes
Atomic force microscopy
biodynamics
membranes
nuclear deformation
atomic force microscopy
Experiments
probes
cells

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Vaziri, A. ; Lee, Hyungsuk ; Kaazempur Mofrad, M. R. / Deformation of the cell nucleus under indentation : Mechanics and mechanisms. In: Journal of Materials Research. 2006 ; Vol. 21, No. 8. pp. 2126-2135.
@article{4f2e73a64f2548b680d6cea521da577b,
title = "Deformation of the cell nucleus under indentation: Mechanics and mechanisms",
abstract = "Computational models of the cell nucleus, along with experimental observations, can help in understanding the biomechanics of force-induced nuclear deformation and mechanisms of stress transition throughout the nucleus. Here, we develop a computational model for an isolated nucleus undergoing indentation, which includes separate components representing the nucleoplasm and the nuclear envelope. The nuclear envelope itself is composed of three separate layers: two thin elastic layers representing the inner and outer nuclear membranes and one thicker layer representing the nuclear lamina. The proposed model is capable of separating the structural role of major nuclear components in the force-induced biological response of the nucleus (and ultimately the cell). A systematic analysis is carried out to explore the role of major individual nuclear elements, namely inner and outer membranes, nuclear lamina, and nucleoplasm, as well as the loading and experimental factors such as indentation rate and probe angle, on the biomechanical response of an isolated nucleus in atomic force microscopy indentation experiment.",
author = "A. Vaziri and Hyungsuk Lee and {Kaazempur Mofrad}, {M. R.}",
year = "2006",
month = "8",
day = "1",
doi = "10.1557/jmr.2006.0262",
language = "English",
volume = "21",
pages = "2126--2135",
journal = "Journal of Materials Research",
issn = "0884-2914",
publisher = "Materials Research Society",
number = "8",

}

Deformation of the cell nucleus under indentation : Mechanics and mechanisms. / Vaziri, A.; Lee, Hyungsuk; Kaazempur Mofrad, M. R.

In: Journal of Materials Research, Vol. 21, No. 8, 01.08.2006, p. 2126-2135.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Deformation of the cell nucleus under indentation

T2 - Mechanics and mechanisms

AU - Vaziri, A.

AU - Lee, Hyungsuk

AU - Kaazempur Mofrad, M. R.

PY - 2006/8/1

Y1 - 2006/8/1

N2 - Computational models of the cell nucleus, along with experimental observations, can help in understanding the biomechanics of force-induced nuclear deformation and mechanisms of stress transition throughout the nucleus. Here, we develop a computational model for an isolated nucleus undergoing indentation, which includes separate components representing the nucleoplasm and the nuclear envelope. The nuclear envelope itself is composed of three separate layers: two thin elastic layers representing the inner and outer nuclear membranes and one thicker layer representing the nuclear lamina. The proposed model is capable of separating the structural role of major nuclear components in the force-induced biological response of the nucleus (and ultimately the cell). A systematic analysis is carried out to explore the role of major individual nuclear elements, namely inner and outer membranes, nuclear lamina, and nucleoplasm, as well as the loading and experimental factors such as indentation rate and probe angle, on the biomechanical response of an isolated nucleus in atomic force microscopy indentation experiment.

AB - Computational models of the cell nucleus, along with experimental observations, can help in understanding the biomechanics of force-induced nuclear deformation and mechanisms of stress transition throughout the nucleus. Here, we develop a computational model for an isolated nucleus undergoing indentation, which includes separate components representing the nucleoplasm and the nuclear envelope. The nuclear envelope itself is composed of three separate layers: two thin elastic layers representing the inner and outer nuclear membranes and one thicker layer representing the nuclear lamina. The proposed model is capable of separating the structural role of major nuclear components in the force-induced biological response of the nucleus (and ultimately the cell). A systematic analysis is carried out to explore the role of major individual nuclear elements, namely inner and outer membranes, nuclear lamina, and nucleoplasm, as well as the loading and experimental factors such as indentation rate and probe angle, on the biomechanical response of an isolated nucleus in atomic force microscopy indentation experiment.

UR - http://www.scopus.com/inward/record.url?scp=33749139567&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749139567&partnerID=8YFLogxK

U2 - 10.1557/jmr.2006.0262

DO - 10.1557/jmr.2006.0262

M3 - Article

VL - 21

SP - 2126

EP - 2135

JO - Journal of Materials Research

JF - Journal of Materials Research

SN - 0884-2914

IS - 8

ER -