Degradation of kidney and Psoas muscle proteins as indicators of post-mortem interval in a rat model, with use of lateral flow technology

Dong Gi Lee, Kyeong Eun Yang, Jeong Won Hwang, Hwan Soo Kang, Seung Yeul Lee, Seoyeon Choi, Joonchul Shin, Ik Soon Jang, Hyun Joo An, Heesun Chung, Hyo Il Jung, Jong Soon Choi

Research output: Contribution to journalArticle

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Abstract

We investigated potential protein markers of post-mortem interval (PMI) using rat kidney and psoas muscle. Tissue samples were taken at 12 h intervals for up to 96 h after death by suffocation. Expression levels of eight soluble proteins were analyzed by Western blotting. Degradation patterns of selected proteins were clearly divided into three groups: short-term, mid-term, and long-term PMI markers based on the half maximum intensity of intact protein expression. In kidney, glycogen synthase (GS) and glycogen synthase kinase-3β were degraded completely within 48 h making them short-term PMI markers. AMP-activated protein kinase α, caspase 3 and GS were short-term PMI markers in psoas muscle. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was a mid-term PMI marker in both tissues. Expression levels of the typical long-term PMI markers, p53 and β-catenin, were constant for at least 96 h post-mortem in both tissues. The degradation patterns of GS and caspase- 3 were verified by immunohistochemistry in both tissues. GAPDH was chosen as a test PMI protein to perform a lateral flow assay (LFA). The presence of recombinant GAPDH was clearly detected in LFA and quantified in a concentration-dependent manner. These results suggest that LFA might be used to estimate PMI at a crime scene.

Original languageEnglish
Article numbere0160557
JournalPloS one
Volume11
Issue number8
DOIs
Publication statusPublished - 2016 Aug

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Psoas Muscles
psoas muscles
glycogen (starch) synthase
Muscle Proteins
muscle protein
glyceraldehyde-3-phosphate dehydrogenase
Rats
animal models
Glycogen Synthase
kidneys
Glyceraldehyde-3-Phosphate Dehydrogenases
Technology
Kidney
Degradation
degradation
Tissue
caspase-3
Assays
Proteins
assays

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Lee, Dong Gi ; Yang, Kyeong Eun ; Hwang, Jeong Won ; Kang, Hwan Soo ; Lee, Seung Yeul ; Choi, Seoyeon ; Shin, Joonchul ; Jang, Ik Soon ; An, Hyun Joo ; Chung, Heesun ; Jung, Hyo Il ; Choi, Jong Soon. / Degradation of kidney and Psoas muscle proteins as indicators of post-mortem interval in a rat model, with use of lateral flow technology. In: PloS one. 2016 ; Vol. 11, No. 8.
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abstract = "We investigated potential protein markers of post-mortem interval (PMI) using rat kidney and psoas muscle. Tissue samples were taken at 12 h intervals for up to 96 h after death by suffocation. Expression levels of eight soluble proteins were analyzed by Western blotting. Degradation patterns of selected proteins were clearly divided into three groups: short-term, mid-term, and long-term PMI markers based on the half maximum intensity of intact protein expression. In kidney, glycogen synthase (GS) and glycogen synthase kinase-3β were degraded completely within 48 h making them short-term PMI markers. AMP-activated protein kinase α, caspase 3 and GS were short-term PMI markers in psoas muscle. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was a mid-term PMI marker in both tissues. Expression levels of the typical long-term PMI markers, p53 and β-catenin, were constant for at least 96 h post-mortem in both tissues. The degradation patterns of GS and caspase- 3 were verified by immunohistochemistry in both tissues. GAPDH was chosen as a test PMI protein to perform a lateral flow assay (LFA). The presence of recombinant GAPDH was clearly detected in LFA and quantified in a concentration-dependent manner. These results suggest that LFA might be used to estimate PMI at a crime scene.",
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Lee, DG, Yang, KE, Hwang, JW, Kang, HS, Lee, SY, Choi, S, Shin, J, Jang, IS, An, HJ, Chung, H, Jung, HI & Choi, JS 2016, 'Degradation of kidney and Psoas muscle proteins as indicators of post-mortem interval in a rat model, with use of lateral flow technology', PloS one, vol. 11, no. 8, e0160557. https://doi.org/10.1371/journal.pone.0160557

Degradation of kidney and Psoas muscle proteins as indicators of post-mortem interval in a rat model, with use of lateral flow technology. / Lee, Dong Gi; Yang, Kyeong Eun; Hwang, Jeong Won; Kang, Hwan Soo; Lee, Seung Yeul; Choi, Seoyeon; Shin, Joonchul; Jang, Ik Soon; An, Hyun Joo; Chung, Heesun; Jung, Hyo Il; Choi, Jong Soon.

In: PloS one, Vol. 11, No. 8, e0160557, 08.2016.

Research output: Contribution to journalArticle

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AU - Lee, Dong Gi

AU - Yang, Kyeong Eun

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AU - Kang, Hwan Soo

AU - Lee, Seung Yeul

AU - Choi, Seoyeon

AU - Shin, Joonchul

AU - Jang, Ik Soon

AU - An, Hyun Joo

AU - Chung, Heesun

AU - Jung, Hyo Il

AU - Choi, Jong Soon

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