Degradation of phenanthrene and naphthalene by a Burkholderia species strain

H. Kang, S. Y. Hwang, Y. M. Kim, E. Kim, Y. S. Kim, S. K. Kim, S. W. Kim, C. E. Cerniglia, K. L. Shuttleworth, G. J. Zylstra

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Abstract

Burkholderia sp. TNFYE-5 was isolated from soil for the ability to grow on phenanthrene as sole carbon and energy source. Unlike most other phenanthrene-degrading bacteria, TNFYE-5 was unable to grow on naphthalene. Growth substrate range experiments coupled with the ring-cleavage enzyme assay data suggest that TNFYE-5 initially metabolizes phenanthrene to 1-hydroxy-2-naphthoate with subsequent degradation through the phthalate and protocatechuate and β-ketoadipate pathway. A metabolite in the degradation of naphthalene by TNFYE-5 was isolated by high-pressure liquid chromatography (HPLC) and was identified as salicylate by UV-visible spectral and gas chromatography - mass spectrometry analyses. Thus, the inability to degrade salicylate is apparently one major reason for the incapability of TNFYE-5 to grow on naphthalene.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalCanadian Journal of Microbiology
Volume49
Issue number2
DOIs
Publication statusPublished - 2003 Feb 1

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics

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    Kang, H., Hwang, S. Y., Kim, Y. M., Kim, E., Kim, Y. S., Kim, S. K., Kim, S. W., Cerniglia, C. E., Shuttleworth, K. L., & Zylstra, G. J. (2003). Degradation of phenanthrene and naphthalene by a Burkholderia species strain. Canadian Journal of Microbiology, 49(2), 139-144. https://doi.org/10.1139/w03-009